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The map label for this gene is nuoB

Identifier: 169629221

GI number: 169629221

Start: 2146555

End: 2147109

Strand: Direct

Name: nuoB

Synonym: MAB_2135

Alternate gene names: 169629221

Gene position: 2146555-2147109 (Clockwise)

Preceding gene: 169629220

Following gene: 169629222

Centisome position: 42.36

GC content: 61.62

Gene sequence:

>555_bases
ATGGGTCTTGAGGAAAAACTGCCGAGCGGATTTCTGCTGAGTACCGTCGAGACACTGGCGGGGTATGTGCGCAAAGGCTC
GCTGTGGCCCGCCACCTTTGGTCTGGCCTGTTGCGCCATCGAGATGATGTCCACGGCGGGCCCCCGATTCGATATCGCTC
GCTTTGGCATGGAACGCTTTTCGGCCACACCGCGCCAGGCGGACCTGATGATCGTGGCAGGCAGGGTGAGCCAGAAGATG
GCCCCGGTGCTGCGCCAGATCTACGACCAGATGGCCGAGCCCAAGTGGGTGTTGGCCATGGGGGTTTGCGCCTCCTCGGG
TGGCATGTTCAATAACTACGCCATCGTCCAGGGCGTCGATCACGTTGTGCCCGTGGATATCTATCTTCCTGGCTGTCCGC
CGCGGCCGGAAATGTTGCTACACGCGATCTTGAAACTGCATGAAAAGATCGCGCACATGCCGCTCGGGGCCAATCGCGAG
GAAGTCATCCGGGAGACCGAAGCCGCCGCATTGGCCGCGACGCCCACCATCGAGATGAAGGGACTGCTGCGGTGA

Upstream 100 bases:

>100_bases
TGGAGGCGCGGTGGCCTGAGCTGGGATTAGCCCCGTGCGATTGATGGGGCAGCTGGAAGTCCTTGCAACATCACGAATAA
GCACGCTTGTCGGGAGAGCC

Downstream 100 bases:

>100_bases
CCGGCGGCGAGCAGAAACCGAATGAAATAATCGGGGTGCGGCGCGGGCTCTTTGGGGTGCGGGGGAGCGGCGACACATCC
GGCTACGGCGGTCTAGTCCA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MGLEEKLPSGFLLSTVETLAGYVRKGSLWPATFGLACCAIEMMSTAGPRFDIARFGMERFSATPRQADLMIVAGRVSQKM
APVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLLHAILKLHEKIAHMPLGANRE
EVIRETEAAALAATPTIEMKGLLR

Sequences:

>Translated_184_residues
MGLEEKLPSGFLLSTVETLAGYVRKGSLWPATFGLACCAIEMMSTAGPRFDIARFGMERFSATPRQADLMIVAGRVSQKM
APVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLLHAILKLHEKIAHMPLGANRE
EVIRETEAAALAATPTIEMKGLLR
>Mature_183_residues
GLEEKLPSGFLLSTVETLAGYVRKGSLWPATFGLACCAIEMMSTAGPRFDIARFGMERFSATPRQADLMIVAGRVSQKMA
PVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLLHAILKLHEKIAHMPLGANREE
VIRETEAAALAATPTIEMKGLLR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=141, Percent_Identity=54.6099290780142, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1788624, Length=150, Percent_Identity=50, Blast_Score=181, Evalue=3e-47,
Organism=Escherichia coli, GI1789074, Length=124, Percent_Identity=38.7096774193548, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788834, Length=112, Percent_Identity=36.6071428571429, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=141, Percent_Identity=53.9007092198582, Blast_Score=173, Evalue=5e-44,
Organism=Drosophila melanogaster, GI18859983, Length=153, Percent_Identity=50.3267973856209, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24642371, Length=153, Percent_Identity=50.3267973856209, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24651058, Length=154, Percent_Identity=51.9480519480519, Blast_Score=180, Evalue=4e-46,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_MYCA9 (B1MPG5)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001702870.1
- ProteinModelPortal:   B1MPG5
- SMR:   B1MPG5
- EnsemblBacteria:   EBMYCT00000007368
- GeneID:   5964648
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2135
- GeneTree:   EBGT00050000016353
- HOGENOM:   HBG553221
- OMA:   CCAIEFM
- ProtClustDB:   PRK06411
- BioCyc:   MABS561007:MAB_2135-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20072; Mature: 19941

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLEEKLPSGFLLSTVETLAGYVRKGSLWPATFGLACCAIEMMSTAGPRFDIARFGMERF
CCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SATPRQADLMIVAGRVSQKMAPVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVD
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCHHHCCCC
HVVPVDIYLPGCPPRPEMLLHAILKLHEKIAHMPLGANREEVIRETEAAALAATPTIEMK
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHC
GLLR
CCCC
>Mature Secondary Structure 
GLEEKLPSGFLLSTVETLAGYVRKGSLWPATFGLACCAIEMMSTAGPRFDIARFGMERF
CCCHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SATPRQADLMIVAGRVSQKMAPVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVD
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCHHHCCCC
HVVPVDIYLPGCPPRPEMLLHAILKLHEKIAHMPLGANREEVIRETEAAALAATPTIEMK
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHC
GLLR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA