Definition Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession NC_010397
Length 5,067,172

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The map label for this gene is mshC

Identifier: 169629202

GI number: 169629202

Start: 2113948

End: 2115186

Strand: Direct

Name: mshC

Synonym: MAB_2116

Alternate gene names: 169629202

Gene position: 2113948-2115186 (Clockwise)

Preceding gene: 169629201

Following gene: 169629203

Centisome position: 41.72

GC content: 66.67

Gene sequence:

>1239_bases
ATGCAGTCGTGGGCGTCGGCGCCGGTTCCTGAACTCGACGGTGGTGGGCCGCAGCTGCGGCTGTATGACACCGCCGACCG
TCAGGTACGTCCGGTCACCACCGGGCCGACCGCGACCATGTACGTGTGTGGCATCACCCCGTATGACGCTACTCATCTGG
GCCATGCCGCAACATATCTCACGTTCGACCTGATCTATCGGCAGTGGCTGGACGCGGGCCTGGACGTGCATTATGTGCAG
AACGTCACCGATATCGATGACCCGCTCTTCGAGCGGGCCGATCGTGACGGCATCGACTGGCGCGAGCTGGGGGACCGGGA
AACAGAGCTGTTTCGTGGCGACATGACCGCGCTGCGGGTGCTACCGCCGCGGGAATATGTGCGTGCCACCGAGTCGATCG
CCTGCATCATCGAGCTCGTCGAGAAGATGCTCGCGTCCGGGGCCGCGTACGTGGTGGACGACCCCGAGTATCCGGACGTG
TACTTCCGCGTCGATGCCACCGAACAGTTCGGCTATGAGTCGGGGTACGACATCGAGACCATGTCGCGGCTTTTTGCCGA
ACGCGGGGGTGATCCAGGCCGTCCCGGCAAGGCCAACGAGCTCGACGCGCTGCTGTGGCGGGCCGCCAGGCCCGGGGAAC
CGAGCTGGGAGGCCTCGTTCGGGCCCGGCCGGCCCGGGTGGCATGTCGAATGCTCCGCGATTGTGCTGCGTGAGTTGGGC
GCCGGTATCGACATCCAGGGGGGCGGCAGCGACCTGATCTTCCCGCACCACGAATACAGCGCCGCGCACGCCGAAGCTGT
TACCGCGCAACGCCGTTTCGCGCGACACTACGTACACGCGGGGATGATCGGGTGGGATGGCCACAAGATGTCCAAGAGCC
GGGGCAATCTGGTGAAGGTATCCGGGCTGACGGCGCAGGGGGTGGACCCCGCCGCGGTAAGGCTCGGGCTGCTGGCTGGT
CACTACCGGGCCGATCGGTCCTGGAGTGATGCGGTGTTGGCTGACGCGCAGGGCAGGCTGGCCCGTTGGCGGCACGCGGT
GGCACTGTCCGCCGCGCCGAGTGCCCGCGACGTGGTGGCCAGAGTCCGCCGCTACCTGGCCGATGACCTTGATACGCCTA
ATGCGCTTGCTGCCCTGGATAACTGGGTGACCGATGCACTGGCATACGGCGGTCACGATGCTGCGGCCGGCGCGCAGGTG
CGCGATGCGGTGGATGCCCTGCTGGGAGTGCAGTTGTAG

Upstream 100 bases:

>100_bases
ATCCGTACCTGCCCGACCTGATCATGTGCCGGCCGGATTTGGCGCCGGTGCTGCTGGACGCGGTCGCCGGGCGCGCCTAA
CGACCCCATAAGGTGTGGGT

Downstream 100 bases:

>100_bases
CGTCGTCGCATGGGTTCAACGGCGAACGGTGTTGCGGGCAAGACGGTCATGATCACCGGCGGTGCCGGCGGAATCGGTGT
CGAGGTGGCGCACCGGCTGT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: L-Cys:GlcN-Ins ligase; Mycothiol ligase; MSH ligase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQSWASAPVPELDGGGPQLRLYDTADRQVRPVTTGPTATMYVCGITPYDATHLGHAATYLTFDLIYRQWLDAGLDVHYVQ
NVTDIDDPLFERADRDGIDWRELGDRETELFRGDMTALRVLPPREYVRATESIACIIELVEKMLASGAAYVVDDPEYPDV
YFRVDATEQFGYESGYDIETMSRLFAERGGDPGRPGKANELDALLWRAARPGEPSWEASFGPGRPGWHVECSAIVLRELG
AGIDIQGGGSDLIFPHHEYSAAHAEAVTAQRRFARHYVHAGMIGWDGHKMSKSRGNLVKVSGLTAQGVDPAAVRLGLLAG
HYRADRSWSDAVLADAQGRLARWRHAVALSAAPSARDVVARVRRYLADDLDTPNALAALDNWVTDALAYGGHDAAAGAQV
RDAVDALLGVQL

Sequences:

>Translated_412_residues
MQSWASAPVPELDGGGPQLRLYDTADRQVRPVTTGPTATMYVCGITPYDATHLGHAATYLTFDLIYRQWLDAGLDVHYVQ
NVTDIDDPLFERADRDGIDWRELGDRETELFRGDMTALRVLPPREYVRATESIACIIELVEKMLASGAAYVVDDPEYPDV
YFRVDATEQFGYESGYDIETMSRLFAERGGDPGRPGKANELDALLWRAARPGEPSWEASFGPGRPGWHVECSAIVLRELG
AGIDIQGGGSDLIFPHHEYSAAHAEAVTAQRRFARHYVHAGMIGWDGHKMSKSRGNLVKVSGLTAQGVDPAAVRLGLLAG
HYRADRSWSDAVLADAQGRLARWRHAVALSAAPSARDVVARVRRYLADDLDTPNALAALDNWVTDALAYGGHDAAAGAQV
RDAVDALLGVQL
>Mature_412_residues
MQSWASAPVPELDGGGPQLRLYDTADRQVRPVTTGPTATMYVCGITPYDATHLGHAATYLTFDLIYRQWLDAGLDVHYVQ
NVTDIDDPLFERADRDGIDWRELGDRETELFRGDMTALRVLPPREYVRATESIACIIELVEKMLASGAAYVVDDPEYPDV
YFRVDATEQFGYESGYDIETMSRLFAERGGDPGRPGKANELDALLWRAARPGEPSWEASFGPGRPGWHVECSAIVLRELG
AGIDIQGGGSDLIFPHHEYSAAHAEAVTAQRRFARHYVHAGMIGWDGHKMSKSRGNLVKVSGLTAQGVDPAAVRLGLLAG
HYRADRSWSDAVLADAQGRLARWRHAVALSAAPSARDVVARVRRYLADDLDTPNALAALDNWVTDALAYGGHDAAAGAQV
RDAVDALLGVQL

Specific function: Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily

Homologues:

Organism=Homo sapiens, GI13375695, Length=387, Percent_Identity=32.8165374677003, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI303304987, Length=279, Percent_Identity=31.1827956989247, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI62240992, Length=279, Percent_Identity=31.1827956989247, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI21269877, Length=279, Percent_Identity=31.1827956989247, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI10835051, Length=237, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786737, Length=385, Percent_Identity=36.6233766233766, Blast_Score=236, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17509795, Length=252, Percent_Identity=30.5555555555556, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17509797, Length=252, Percent_Identity=30.5555555555556, Blast_Score=118, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324082, Length=365, Percent_Identity=27.6712328767123, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19922218, Length=386, Percent_Identity=32.9015544041451, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI19922364, Length=229, Percent_Identity=31.0043668122271, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHC_MYCA9 (B1MPE6)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001702851.1
- ProteinModelPortal:   B1MPE6
- SMR:   B1MPE6
- EnsemblBacteria:   EBMYCT00000008260
- GeneID:   5964629
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2116
- GeneTree:   EBGT00050000016491
- HOGENOM:   HBG327651
- OMA:   ALFREDM
- ProtClustDB:   PRK12418
- BioCyc:   MABS561007:MAB_2116-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01697
- InterPro:   IPR015803
- InterPro:   IPR017812
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR03447

Pfam domain/function: PF01406 tRNA-synt_1e

EC number: =6.3.1.13

Molecular weight: Translated: 45010; Mature: 45010

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: BINDING 58-58 BINDING 227-227 BINDING 283-283

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MQSWASAPVPELDGGGPQLRLYDTADRQVRPVTTGPTATMYVCGITPYDATHLGHAATYL
CCCCCCCCCCCCCCCCCEEEEEECCCCEEECEECCCCEEEEEEECCCCCHHHHHHHHHHH
TFDLIYRQWLDAGLDVHYVQNVTDIDDPLFERADRDGIDWRELGDRETELFRGDMTALRV
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCCCCHHHCCCHHHHHHHCCCCEEEE
LPPREYVRATESIACIIELVEKMLASGAAYVVDDPEYPDVYFRVDATEQFGYESGYDIET
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECHHHCCCCCCCCHHH
MSRLFAERGGDPGRPGKANELDALLWRAARPGEPSWEASFGPGRPGWHVECSAIVLRELG
HHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHC
AGIDIQGGGSDLIFPHHEYSAAHAEAVTAQRRFARHYVHAGMIGWDGHKMSKSRGNLVKV
CCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEE
SGLTAQGVDPAAVRLGLLAGHYRADRSWSDAVLADAQGRLARWRHAVALSAAPSARDVVA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHEEECCCCHHHHHH
RVRRYLADDLDTPNALAALDNWVTDALAYGGHDAAAGAQVRDAVDALLGVQL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSWASAPVPELDGGGPQLRLYDTADRQVRPVTTGPTATMYVCGITPYDATHLGHAATYL
CCCCCCCCCCCCCCCCCEEEEEECCCCEEECEECCCCEEEEEEECCCCCHHHHHHHHHHH
TFDLIYRQWLDAGLDVHYVQNVTDIDDPLFERADRDGIDWRELGDRETELFRGDMTALRV
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCCCCHHHCCCHHHHHHHCCCCEEEE
LPPREYVRATESIACIIELVEKMLASGAAYVVDDPEYPDVYFRVDATEQFGYESGYDIET
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECHHHCCCCCCCCHHH
MSRLFAERGGDPGRPGKANELDALLWRAARPGEPSWEASFGPGRPGWHVECSAIVLRELG
HHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHC
AGIDIQGGGSDLIFPHHEYSAAHAEAVTAQRRFARHYVHAGMIGWDGHKMSKSRGNLVKV
CCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEE
SGLTAQGVDPAAVRLGLLAGHYRADRSWSDAVLADAQGRLARWRHAVALSAAPSARDVVA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHEEECCCCHHHHHH
RVRRYLADDLDTPNALAALDNWVTDALAYGGHDAAAGAQVRDAVDALLGVQL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA