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The map label for this gene is fadD [C]

Identifier: 169628782

GI number: 169628782

Start: 1717514

End: 1719196

Strand: Direct

Name: fadD [C]

Synonym: MAB_1692

Alternate gene names: 169628782

Gene position: 1717514-1719196 (Clockwise)

Preceding gene: 169628781

Following gene: 169628783

Centisome position: 33.89

GC content: 67.38

Gene sequence:

>1683_bases
ATGAGCCGCTTGCGCGAAGACCGTCCTGGGTCGAACCCCGTCATCGACCGCCTGCTGGCCGGGGCCGGTATCACCGGCCG
CGGCCTCACCTCGGCGCCGATAGGCCCCGACGGCGCATCGCAGCTGGATGAGTTGAGCACCACGGCGTGGGCGGACGTGC
ACCGGACGGGGCTGCGTATCGCAGGTGCCCTGCAGGCAGCAGGTGTGCGGCCCGGTGATGCCGTCGCGGTATTGGCCGGT
GCCCCTGGGGAAATCGCGCCACTGGTACAGGGCATCTGGTTCTCCGGTGCCTCGGTCACCATGTTGCATCAGCCGACCCA
TCGCTCCGATATGCGGCTCTGGGTGGAGGACACCGGCCGGGCACTGGCCGCAGTCGGCTCTTCGACGGTCGTGGTGGGTG
ATCCCTTCATGGCCGCGGCGAGCCAGTTCGGCGCTGTCGCCGCACAGGTTCTGGAGATGTCCGCGCTGCTGGATGGTCCG
CCCGGCGAGATTGTGCAGAGTGATCAGGACGCTATCGCGTTCTTACAGTTGACCTCTGGATCCACCGGCACACCGAAGGC
CGTCAGCATCAGCTATCGCAATATCGAGGCCAATCTGCGGGCCATGGTGAGCGCGTCCGGCGCGGATCCCGAAACAGATG
TGGTGGTGAGTTGGCTGCCGCTGTTCCACGACATGGGCATGATGGGATTTCTCATCGTGCCCATGTGCCGGGCCATGAGC
ACCGTCAGCGTGACCCCCATGGACTTCCTCGGTGATCCGCTGTTGTGGGCCAAGCTCATCAGCACCTACCGGGGCAGCAT
GACGGCGGGTCCCAACTTCGCCTACTCGCTGCTGGCCCGCCGCCTGCGCAAGGCGCCCGACGGCGCATACGACCTGTCCT
CGCTGCAATTCGCGCTCAGCGGTGCCGAGGCCATCGATGTCGCGACCCTGGAACGGTTCGTCGCCGAGGGCGCCCGCTTC
GGCATGCGTGCGGACGCCGTCGTCCCCGCCTACGGGATGGCGGAAGCCACCCTGGCGGTCTCGTTTGTGCGGCGCGGCGA
GGGCTATCGGGTGTGCCCGCCGCATCCCGATCTCGTTGCGGCGCAGGGTACGCAGGCCAGCGCCCGTGTCCCACTGGGGC
TACCGCTGCCCGGGTGCGAGGTACGGGTGGTCACCGAGGACCGCCAGGTGTTGGCTCCGGAATCGATCGGCGAGCTCGAG
ATCCGCGGTGACAACGTCACCGCGGGATACCGCACGGCATCCGGATTCGAGTCGATGCTCGATGCGCAAGGCTGGCTCTC
CACCGGCGATATCGGCTATCTCACCGCGGACGGCCAGCCCGTGATCTGTGGCCGCAAGAAAGACATCCTGATCATCTCCG
GGCGTAACGTGCATCCGGAGGACATCGAACGCTCGGTGGCCGGAATATCCGGGGTGCGTTCCGGTGGCGTGGCTGCCGTG
CGCCTGCACACCGCTTCCGCCGGCGAGGGATTCGCGATGGTGGCGGAATCGGCCCTGCATGCCGATATCGCCGAGAGCGA
GCGCATCCGCGCGGAAGTGGCCGACCGCGTCTACCGCACCCTGGGGGTGAGCCCACGCGATGTCCATGTGGTTCCCGCCG
GATGGATTCCCAAGACGTCCTCGGGGAAGCTGCGCCGGAGGGCCACCTCTGAACGGCTGAAGAGGTGTGAACCGGCCCGA
TGA

Upstream 100 bases:

>100_bases
CGGAGATCAAGGGCCGCTTCGGCATTCAGTTGACCAAGGATGATGTCTTCGAATGCAAGACCTTGGGTGCGCTGATCGAG
CTGGTGGAGAGTCGGCTGTG

Downstream 100 bases:

>100_bases
CGACACGGGATCAACTAGTCCGTGTCGGCGGCCAGATGCCCAAGAGGATTCGCACACTTGGTGCCTGCGGCGCGGGATAT
GTGCGGCACCACCCGATCGC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSRLREDRPGSNPVIDRLLAGAGITGRGLTSAPIGPDGASQLDELSTTAWADVHRTGLRIAGALQAAGVRPGDAVAVLAG
APGEIAPLVQGIWFSGASVTMLHQPTHRSDMRLWVEDTGRALAAVGSSTVVVGDPFMAAASQFGAVAAQVLEMSALLDGP
PGEIVQSDQDAIAFLQLTSGSTGTPKAVSISYRNIEANLRAMVSASGADPETDVVVSWLPLFHDMGMMGFLIVPMCRAMS
TVSVTPMDFLGDPLLWAKLISTYRGSMTAGPNFAYSLLARRLRKAPDGAYDLSSLQFALSGAEAIDVATLERFVAEGARF
GMRADAVVPAYGMAEATLAVSFVRRGEGYRVCPPHPDLVAAQGTQASARVPLGLPLPGCEVRVVTEDRQVLAPESIGELE
IRGDNVTAGYRTASGFESMLDAQGWLSTGDIGYLTADGQPVICGRKKDILIISGRNVHPEDIERSVAGISGVRSGGVAAV
RLHTASAGEGFAMVAESALHADIAESERIRAEVADRVYRTLGVSPRDVHVVPAGWIPKTSSGKLRRRATSERLKRCEPAR

Sequences:

>Translated_560_residues
MSRLREDRPGSNPVIDRLLAGAGITGRGLTSAPIGPDGASQLDELSTTAWADVHRTGLRIAGALQAAGVRPGDAVAVLAG
APGEIAPLVQGIWFSGASVTMLHQPTHRSDMRLWVEDTGRALAAVGSSTVVVGDPFMAAASQFGAVAAQVLEMSALLDGP
PGEIVQSDQDAIAFLQLTSGSTGTPKAVSISYRNIEANLRAMVSASGADPETDVVVSWLPLFHDMGMMGFLIVPMCRAMS
TVSVTPMDFLGDPLLWAKLISTYRGSMTAGPNFAYSLLARRLRKAPDGAYDLSSLQFALSGAEAIDVATLERFVAEGARF
GMRADAVVPAYGMAEATLAVSFVRRGEGYRVCPPHPDLVAAQGTQASARVPLGLPLPGCEVRVVTEDRQVLAPESIGELE
IRGDNVTAGYRTASGFESMLDAQGWLSTGDIGYLTADGQPVICGRKKDILIISGRNVHPEDIERSVAGISGVRSGGVAAV
RLHTASAGEGFAMVAESALHADIAESERIRAEVADRVYRTLGVSPRDVHVVPAGWIPKTSSGKLRRRATSERLKRCEPAR
>Mature_559_residues
SRLREDRPGSNPVIDRLLAGAGITGRGLTSAPIGPDGASQLDELSTTAWADVHRTGLRIAGALQAAGVRPGDAVAVLAGA
PGEIAPLVQGIWFSGASVTMLHQPTHRSDMRLWVEDTGRALAAVGSSTVVVGDPFMAAASQFGAVAAQVLEMSALLDGPP
GEIVQSDQDAIAFLQLTSGSTGTPKAVSISYRNIEANLRAMVSASGADPETDVVVSWLPLFHDMGMMGFLIVPMCRAMST
VSVTPMDFLGDPLLWAKLISTYRGSMTAGPNFAYSLLARRLRKAPDGAYDLSSLQFALSGAEAIDVATLERFVAEGARFG
MRADAVVPAYGMAEATLAVSFVRRGEGYRVCPPHPDLVAAQGTQASARVPLGLPLPGCEVRVVTEDRQVLAPESIGELEI
RGDNVTAGYRTASGFESMLDAQGWLSTGDIGYLTADGQPVICGRKKDILIISGRNVHPEDIERSVAGISGVRSGGVAAVR
LHTASAGEGFAMVAESALHADIAESERIRAEVADRVYRTLGVSPRDVHVVPAGWIPKTSSGKLRRRATSERLKRCEPAR

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=388, Percent_Identity=27.8350515463918, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI145693145, Length=389, Percent_Identity=27.5064267352185, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=548, Percent_Identity=24.2700729927007, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=321, Percent_Identity=28.6604361370716, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=406, Percent_Identity=24.6305418719212, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=399, Percent_Identity=23.8095238095238, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21355181, Length=395, Percent_Identity=27.5949367088608, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859661, Length=398, Percent_Identity=24.6231155778894, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356441, Length=456, Percent_Identity=23.2456140350877, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648253, Length=436, Percent_Identity=22.9357798165138, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161076582, Length=529, Percent_Identity=22.8733459357278, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648255, Length=436, Percent_Identity=22.9357798165138, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653035, Length=490, Percent_Identity=22.8571428571429, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21356947, Length=516, Percent_Identity=24.6124031007752, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648257, Length=393, Percent_Identity=24.4274809160305, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58887; Mature: 58756

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLREDRPGSNPVIDRLLAGAGITGRGLTSAPIGPDGASQLDELSTTAWADVHRTGLRI
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHH
AGALQAAGVRPGDAVAVLAGAPGEIAPLVQGIWFSGASVTMLHQPTHRSDMRLWVEDTGR
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCC
ALAAVGSSTVVVGDPFMAAASQFGAVAAQVLEMSALLDGPPGEIVQSDQDAIAFLQLTSG
EEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEEEECC
STGTPKAVSISYRNIEANLRAMVSASGADPETDVVVSWLPLFHDMGMMGFLIVPMCRAMS
CCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVSVTPMDFLGDPLLWAKLISTYRGSMTAGPNFAYSLLARRLRKAPDGAYDLSSLQFALS
CCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
GAEAIDVATLERFVAEGARFGMRADAVVPAYGMAEATLAVSFVRRGEGYRVCPPHPDLVA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCEEE
AQGTQASARVPLGLPLPGCEVRVVTEDRQVLAPESIGELEIRGDNVTAGYRTASGFESML
ECCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCEEEECCCEECCCCCHHHHHHHH
DAQGWLSTGDIGYLTADGQPVICGRKKDILIISGRNVHPEDIERSVAGISGVRSGGVAAV
HHCCCCCCCCEEEEECCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEE
RLHTASAGEGFAMVAESALHADIAESERIRAEVADRVYRTLGVSPRDVHVVPAGWIPKTS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
SGKLRRRATSERLKRCEPAR
CCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRLREDRPGSNPVIDRLLAGAGITGRGLTSAPIGPDGASQLDELSTTAWADVHRTGLRI
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHH
AGALQAAGVRPGDAVAVLAGAPGEIAPLVQGIWFSGASVTMLHQPTHRSDMRLWVEDTGR
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCC
ALAAVGSSTVVVGDPFMAAASQFGAVAAQVLEMSALLDGPPGEIVQSDQDAIAFLQLTSG
EEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEEEECC
STGTPKAVSISYRNIEANLRAMVSASGADPETDVVVSWLPLFHDMGMMGFLIVPMCRAMS
CCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVSVTPMDFLGDPLLWAKLISTYRGSMTAGPNFAYSLLARRLRKAPDGAYDLSSLQFALS
CCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
GAEAIDVATLERFVAEGARFGMRADAVVPAYGMAEATLAVSFVRRGEGYRVCPPHPDLVA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCEEE
AQGTQASARVPLGLPLPGCEVRVVTEDRQVLAPESIGELEIRGDNVTAGYRTASGFESML
ECCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCEEEECCCEECCCCCHHHHHHHH
DAQGWLSTGDIGYLTADGQPVICGRKKDILIISGRNVHPEDIERSVAGISGVRSGGVAAV
HHCCCCCCCCEEEEECCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEE
RLHTASAGEGFAMVAESALHADIAESERIRAEVADRVYRTLGVSPRDVHVVPAGWIPKTS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
SGKLRRRATSERLKRCEPAR
CCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA