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The map label for this gene is lepA [H]

Identifier: 169628730

GI number: 169628730

Start: 1669151

End: 1671004

Strand: Direct

Name: lepA [H]

Synonym: MAB_1640

Alternate gene names: 169628730

Gene position: 1669151-1671004 (Clockwise)

Preceding gene: 169628728

Following gene: 169628731

Centisome position: 32.94

GC content: 64.13

Gene sequence:

>1854_bases
GTGCCAAGTTTTGCCGACACGACGTTCACGGATCCGGCGCTGATTCGTAACTTCTGCATCATCGCCCACATCGACCACGG
GAAGTCGACCCTGGCCGACCGGATGCTGCAGCTGACCGGTGTGGTCGACGAGCGTTCCATGCGCGCGCAGTACCTGGATC
GCATGGATATCGAGCGTGAGCGCGGCATCACCATCAAGGCACAGAACGTGCGGCTGCCGTGGCAGCTCAATGGCCAGGAC
CATGTGCTACACCTGATCGACACCCCCGGGCACGTCGACTTCACCTACGAGGTGTCGCGCGCCCTGGAGGCGTGCGAGGG
GGCAGTGCTGCTGGTCGACGCCGCGCAGGGCATCGAAGCGCAGACGCTGGCCAACCTGTACCTGGCGCTGGACAAGGACC
TGACGATCATCCCGGTCCTGAACAAGATCGACCTGCCCGCGGCCGATCCGGAGCGTTACGCCGAGGAGATCGCGCACATC
ATCGGCTGCGAGCCCTCCGATGTGCTGCGGGTGTCCGGTAAGACCGGGGAGGGCGTCGCGGCGCTGCTCGACGAGGTGGT
GCGGCTCATCCCAGCGCCGCAAGGTGATCCGGATGCGCCGGCGCGGGCGATGATCTTCGACTCGGTCTACGACATCTACC
GCGGTGTGGTCACCTACGTGCGCGTCGTGGACGGCAAGATCACTCCGCGCGAGAAGATCGCGATGATGTCGACGGGCGCC
ACCCACGAACTGCTGGAAGTGGGCATCGTCTCGCCGGAGCCCAAACCCTCCGCGGGACTCGGGGTCGGCGAGGTGGGCTA
CCTGATCACCGGCGTGAAGGATGTGCGCCAGTCGAAGGTCGGTGACACCGTCACCGCCGCGCGCAACGGCGCCAAGGAGC
CGCTGACCGGCTACCGCGAACCCAAGCCGATGGTCTATTCCGGGCTGTACCCGGTGGACGGATCCGACTACCCGAACCTG
CGCGAGGCGCTGGACAAACTCCAGCTCAACGACGCCGCGCTCACCTACGAGCCGGAGACCTCGGTGGCGCTGGGCTTCGG
ATTCCGTTGTGGCTTCCTGGGATTGCTGCACATGGAGATCACCCGTGAGCGCCTCGAACGCGAGTTCAACCTCGACCTCA
TCTCGACGGCTCCCAACGTGGTGTACCGGGTGATTACCGAAGGAATGACGGCCGATGATCCAGGGATGATGGTGACCAAT
CCGTCCATCTGGCCCGAGGGCAAGATCCGGTCGGTGTTCGAGCCGGTGGTCAAGACGACGGTGATCGCGCCGAGTGAATT
CATCGGTTCGATCATGGAGCTGTGCCAATCGCGGCGCGGCGAGCTCGGCGGCATGGACTACCTGTCACCCGAACGCGTCG
AGCTGCGCTACACGATGCCGTTGGGCGAGATCATCTTCGACTTCTTCGACTCGTTGAAGTCGCGTACGCGCGGCTACGCC
AGCCTGGATTACGAGGAGGCCGGCGAGCAGGAGGCCGATCTGGTCAAGGTCGACATCCTGCTGCAGGGCGAGGCGGTCGA
CGCGTTCAGCGCCATCGTCCACAAGGACGGGGCCTCGGCCTATGGCAACAAGATGACCGTCAAGCTCAAGGAACTCATCC
CGCGCCAGCAGTTCGAGGTGCCGGTGCAGGCCGCTGTCGGATCGAGAATCATTGCGCGCGAGAATATTCGGGCCATCCGT
AAGGATGTGCTCTCCAAGTGCTACGGCGGTGATATCACCCGTAAGCGCAAGCTGCTCGAGAAGCAGAAGGAGGGCAAGAA
GCGCATGAAGACCATCGGTCGCGTCGAGGTGCCGCAGGAGGCGTTTGTGGCGGCACTGTCCACCGAGTCGACGGCGGACA
AACCGAAGAAGTAG

Upstream 100 bases:

>100_bases
AGCGTCTGCCACTGTGACGCCATGGTGCGAGCATACTGACCGGTAGTCCCTCACGACGTGCGTGTCAGAAGCATGCCGCG
CGGCATGGGAGCATGGTGCC

Downstream 100 bases:

>100_bases
CGGTACCTCCCGAACAAGCGCGGTACCACCGCGAGCATGCTCAAATACCCCGTTTCAGATCGATTTCAGGCACATTTGAG
CATGTTCGCGCCGGAGGTGT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MPSFADTTFTDPALIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSMRAQYLDRMDIERERGITIKAQNVRLPWQLNGQD
HVLHLIDTPGHVDFTYEVSRALEACEGAVLLVDAAQGIEAQTLANLYLALDKDLTIIPVLNKIDLPAADPERYAEEIAHI
IGCEPSDVLRVSGKTGEGVAALLDEVVRLIPAPQGDPDAPARAMIFDSVYDIYRGVVTYVRVVDGKITPREKIAMMSTGA
THELLEVGIVSPEPKPSAGLGVGEVGYLITGVKDVRQSKVGDTVTAARNGAKEPLTGYREPKPMVYSGLYPVDGSDYPNL
REALDKLQLNDAALTYEPETSVALGFGFRCGFLGLLHMEITRERLEREFNLDLISTAPNVVYRVITEGMTADDPGMMVTN
PSIWPEGKIRSVFEPVVKTTVIAPSEFIGSIMELCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDSLKSRTRGYA
SLDYEEAGEQEADLVKVDILLQGEAVDAFSAIVHKDGASAYGNKMTVKLKELIPRQQFEVPVQAAVGSRIIARENIRAIR
KDVLSKCYGGDITRKRKLLEKQKEGKKRMKTIGRVEVPQEAFVAALSTESTADKPKK

Sequences:

>Translated_617_residues
MPSFADTTFTDPALIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSMRAQYLDRMDIERERGITIKAQNVRLPWQLNGQD
HVLHLIDTPGHVDFTYEVSRALEACEGAVLLVDAAQGIEAQTLANLYLALDKDLTIIPVLNKIDLPAADPERYAEEIAHI
IGCEPSDVLRVSGKTGEGVAALLDEVVRLIPAPQGDPDAPARAMIFDSVYDIYRGVVTYVRVVDGKITPREKIAMMSTGA
THELLEVGIVSPEPKPSAGLGVGEVGYLITGVKDVRQSKVGDTVTAARNGAKEPLTGYREPKPMVYSGLYPVDGSDYPNL
REALDKLQLNDAALTYEPETSVALGFGFRCGFLGLLHMEITRERLEREFNLDLISTAPNVVYRVITEGMTADDPGMMVTN
PSIWPEGKIRSVFEPVVKTTVIAPSEFIGSIMELCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDSLKSRTRGYA
SLDYEEAGEQEADLVKVDILLQGEAVDAFSAIVHKDGASAYGNKMTVKLKELIPRQQFEVPVQAAVGSRIIARENIRAIR
KDVLSKCYGGDITRKRKLLEKQKEGKKRMKTIGRVEVPQEAFVAALSTESTADKPKK
>Mature_616_residues
PSFADTTFTDPALIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSMRAQYLDRMDIERERGITIKAQNVRLPWQLNGQDH
VLHLIDTPGHVDFTYEVSRALEACEGAVLLVDAAQGIEAQTLANLYLALDKDLTIIPVLNKIDLPAADPERYAEEIAHII
GCEPSDVLRVSGKTGEGVAALLDEVVRLIPAPQGDPDAPARAMIFDSVYDIYRGVVTYVRVVDGKITPREKIAMMSTGAT
HELLEVGIVSPEPKPSAGLGVGEVGYLITGVKDVRQSKVGDTVTAARNGAKEPLTGYREPKPMVYSGLYPVDGSDYPNLR
EALDKLQLNDAALTYEPETSVALGFGFRCGFLGLLHMEITRERLEREFNLDLISTAPNVVYRVITEGMTADDPGMMVTNP
SIWPEGKIRSVFEPVVKTTVIAPSEFIGSIMELCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDSLKSRTRGYAS
LDYEEAGEQEADLVKVDILLQGEAVDAFSAIVHKDGASAYGNKMTVKLKELIPRQQFEVPVQAAVGSRIIARENIRAIRK
DVLSKCYGGDITRKRKLLEKQKEGKKRMKTIGRVEVPQEAFVAALSTESTADKPKK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=48.3388704318937, Blast_Score=621, Evalue=1e-178,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=40.7407407407407, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI18390331, Length=149, Percent_Identity=36.9127516778523, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI25306287, Length=158, Percent_Identity=40.5063291139241, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310132016, Length=112, Percent_Identity=37.5, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI310110807, Length=112, Percent_Identity=37.5, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI310123363, Length=112, Percent_Identity=37.5, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI25306283, Length=158, Percent_Identity=40.5063291139241, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI19923640, Length=158, Percent_Identity=40.5063291139241, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=36.551724137931, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI53729339, Length=281, Percent_Identity=29.5373665480427, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI53729337, Length=281, Percent_Identity=29.5373665480427, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI217272892, Length=135, Percent_Identity=34.0740740740741, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI217272894, Length=135, Percent_Identity=34.0740740740741, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI5729864, Length=267, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI223555963, Length=267, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI4503471, Length=331, Percent_Identity=27.190332326284, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI4503475, Length=331, Percent_Identity=27.190332326284, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=54.3771043771044, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=480, Percent_Identity=28.5416666666667, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790835, Length=189, Percent_Identity=31.7460317460317, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=35.8490566037736, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789559, Length=284, Percent_Identity=27.8169014084507, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=617, Percent_Identity=41.0048622366288, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=36.1290322580645, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=137, Percent_Identity=39.4160583941606, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=143, Percent_Identity=40.5594405594406, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=137, Percent_Identity=39.4160583941606, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=168, Percent_Identity=32.1428571428571, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=148, Percent_Identity=33.1081081081081, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=44.8504983388704, Blast_Score=527, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6323098, Length=188, Percent_Identity=32.9787234042553, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=37.7622377622378, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=37.7622377622378, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=159, Percent_Identity=39.622641509434, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=43.859649122807, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325337, Length=329, Percent_Identity=26.1398176291793, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=329, Percent_Identity=26.1398176291793, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78706572, Length=597, Percent_Identity=44.0536013400335, Blast_Score=537, Evalue=1e-153,
Organism=Drosophila melanogaster, GI28574573, Length=136, Percent_Identity=43.3823529411765, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24582462, Length=144, Percent_Identity=40.2777777777778, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221458488, Length=173, Percent_Identity=38.150289017341, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585709, Length=160, Percent_Identity=33.75, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585711, Length=160, Percent_Identity=33.75, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585713, Length=160, Percent_Identity=33.75, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357743, Length=138, Percent_Identity=37.6811594202899, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 68027; Mature: 67896

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFADTTFTDPALIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSMRAQYLDRMDIERE
CCCCCCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHH
RGITIKAQNVRLPWQLNGQDHVLHLIDTPGHVDFTYEVSRALEACEGAVLLVDAAQGIEA
CCCEEEECCEECCEEECCCCCEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCH
QTLANLYLALDKDLTIIPVLNKIDLPAADPERYAEEIAHIIGCEPSDVLRVSGKTGEGVA
HHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHH
ALLDEVVRLIPAPQGDPDAPARAMIFDSVYDIYRGVVTYVRVVDGKITPREKIAMMSTGA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCC
THELLEVGIVSPEPKPSAGLGVGEVGYLITGVKDVRQSKVGDTVTAARNGAKEPLTGYRE
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCC
PKPMVYSGLYPVDGSDYPNLREALDKLQLNDAALTYEPETSVALGFGFRCGFLGLLHMEI
CCCCEEECEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHH
TRERLEREFNLDLISTAPNVVYRVITEGMTADDPGMMVTNPSIWPEGKIRSVFEPVVKTT
HHHHHHHHCCCEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
VIAPSEFIGSIMELCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDSLKSRTRGYA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCE
SLDYEEAGEQEADLVKVDILLQGEAVDAFSAIVHKDGASAYGNKMTVKLKELIPRQQFEV
ECCHHHCCCCCCCEEEEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCHHCCC
PVQAAVGSRIIARENIRAIRKDVLSKCYGGDITRKRKLLEKQKEGKKRMKTIGRVEVPQE
CHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AFVAALSTESTADKPKK
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PSFADTTFTDPALIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSMRAQYLDRMDIERE
CCCCCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHH
RGITIKAQNVRLPWQLNGQDHVLHLIDTPGHVDFTYEVSRALEACEGAVLLVDAAQGIEA
CCCEEEECCEECCEEECCCCCEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCH
QTLANLYLALDKDLTIIPVLNKIDLPAADPERYAEEIAHIIGCEPSDVLRVSGKTGEGVA
HHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHH
ALLDEVVRLIPAPQGDPDAPARAMIFDSVYDIYRGVVTYVRVVDGKITPREKIAMMSTGA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCC
THELLEVGIVSPEPKPSAGLGVGEVGYLITGVKDVRQSKVGDTVTAARNGAKEPLTGYRE
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCC
PKPMVYSGLYPVDGSDYPNLREALDKLQLNDAALTYEPETSVALGFGFRCGFLGLLHMEI
CCCCEEECEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHH
TRERLEREFNLDLISTAPNVVYRVITEGMTADDPGMMVTNPSIWPEGKIRSVFEPVVKTT
HHHHHHHHCCCEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
VIAPSEFIGSIMELCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDSLKSRTRGYA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCE
SLDYEEAGEQEADLVKVDILLQGEAVDAFSAIVHKDGASAYGNKMTVKLKELIPRQQFEV
ECCHHHCCCCCCCEEEEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCHHCCC
PVQAAVGSRIIARENIRAIRKDVLSKCYGGDITRKRKLLEKQKEGKKRMKTIGRVEVPQE
CHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AFVAALSTESTADKPKK
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA