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The map label for this gene is proB [H]

Identifier: 169628703

GI number: 169628703

Start: 1643600

End: 1644706

Strand: Direct

Name: proB [H]

Synonym: MAB_1613

Alternate gene names: 169628703

Gene position: 1643600-1644706 (Clockwise)

Preceding gene: 169628702

Following gene: 169628704

Centisome position: 32.44

GC content: 67.12

Gene sequence:

>1107_bases
GTGAATCCAGGTGCGCAGGCGCGTGAGGCCATTCGCTCGGCGCGCACCGTCGTGGTGAAGGTCGGCACCGCCGCACTCAC
CGATGAGACGGGGACCTTCAGTGCCGACCGCATGGAATACCTCGTCGAGGTCATCGAAGGCCGGATGAAGGCTGGATCCG
ATGTGGTGATCGTGTCCTCCGGGGCGATCGCGGCGGGCATGGTTCCGCTCGGATTGACCAAGCGCCCAACGGATCTGGCG
ACCAAGCAGGCGGCGGCCAGCGCTGGTCAAGTGGCCCTTATCGGCGCCTGGAGTGCGGCCTTCGCGCGTTATGAGCGCAC
CGTGGGCCAGGTGCTGCTGACCGCCCACGACATCTCGATGCGCGTGCACCACACCAACGCGCAGCGCACCCTCGACAGAC
TCACCGCATTGCACGCGGTTGCCATCGTGAATGAGAACGACACCGTGGCTACCAACGAGATTCGTTTCGGAGACAATGAC
CGCCTCTCGGCGCTTGTCGCGCATTTGGTGGGCGCCGATGCGCTGGTGTTGCTCTCCGACATCGATGGACTCTACGATTC
AGACCCCCGAAAGGGGAACGCGCGCTTCATTTCTGAGGTGAGCGGCCCCGAGGACCTGGCCGACGTGATCGCCGGCCCTG
GCGGTTCGCGTGGTACTGGAGGAATGGCCTCCAAGCTCTCCTCGGCGCTGCTGGCTTCCGACGCCGGTGTGCCGGTGCTC
TTGGCCTCGGCGACAGAGGCGGCGGCGGCACTGGGAGACGGCTCTTGCGGAACCGTTTTCGCCGCGCGGCCGCAACGCAT
GTCCGCCCGCAAGTTCTGGGTGCGTTACGCCGCCGAGGCCACCGGTTCACTGACGCTGGACGCCGGCGCGGTACGCGCGG
TCGTGGCGTCCCGGCGTTCGTTGCTGCCTGCCGGTATCACCGGGGTGTCCGGACGCTTCCACGGGGGCGATGTCGTTGAG
CTCCGCGACGCGGACGGCACCATGGTCGGCCGGGGTGTCGTCGCCTATGACGTAGCTGAATTGGCGACCATGCTGGGCCG
CTCCACCAACGAACTCCCCGAGGATCTACGGCGCCCCGCCGTGCACGCCGACGACCTCGTCACCTGA

Upstream 100 bases:

>100_bases
GCGGACGCGGTACCGACGCGCGCATCGACAAGACAGATCGGGTGGGTGCGGCCGAGCGCCGCCAGGCCAGGCGAGTGCGA
CGTGGGCAGGTGGAGCCGGA

Downstream 100 bases:

>100_bases
GCGGTTCACGCATTTGCTCGAAAGAGTGGCACATCGCAACGGCGCCTGATAGAAACGTTCAGCTAGTTTCCAGCTTGGGG
GTGTGCCCTGGGCTTCGTGT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNPGAQAREAIRSARTVVVKVGTAALTDETGTFSADRMEYLVEVIEGRMKAGSDVVIVSSGAIAAGMVPLGLTKRPTDLA
TKQAAASAGQVALIGAWSAAFARYERTVGQVLLTAHDISMRVHHTNAQRTLDRLTALHAVAIVNENDTVATNEIRFGDND
RLSALVAHLVGADALVLLSDIDGLYDSDPRKGNARFISEVSGPEDLADVIAGPGGSRGTGGMASKLSSALLASDAGVPVL
LASATEAAAALGDGSCGTVFAARPQRMSARKFWVRYAAEATGSLTLDAGAVRAVVASRRSLLPAGITGVSGRFHGGDVVE
LRDADGTMVGRGVVAYDVAELATMLGRSTNELPEDLRRPAVHADDLVT

Sequences:

>Translated_368_residues
MNPGAQAREAIRSARTVVVKVGTAALTDETGTFSADRMEYLVEVIEGRMKAGSDVVIVSSGAIAAGMVPLGLTKRPTDLA
TKQAAASAGQVALIGAWSAAFARYERTVGQVLLTAHDISMRVHHTNAQRTLDRLTALHAVAIVNENDTVATNEIRFGDND
RLSALVAHLVGADALVLLSDIDGLYDSDPRKGNARFISEVSGPEDLADVIAGPGGSRGTGGMASKLSSALLASDAGVPVL
LASATEAAAALGDGSCGTVFAARPQRMSARKFWVRYAAEATGSLTLDAGAVRAVVASRRSLLPAGITGVSGRFHGGDVVE
LRDADGTMVGRGVVAYDVAELATMLGRSTNELPEDLRRPAVHADDLVT
>Mature_368_residues
MNPGAQAREAIRSARTVVVKVGTAALTDETGTFSADRMEYLVEVIEGRMKAGSDVVIVSSGAIAAGMVPLGLTKRPTDLA
TKQAAASAGQVALIGAWSAAFARYERTVGQVLLTAHDISMRVHHTNAQRTLDRLTALHAVAIVNENDTVATNEIRFGDND
RLSALVAHLVGADALVLLSDIDGLYDSDPRKGNARFISEVSGPEDLADVIAGPGGSRGTGGMASKLSSALLASDAGVPVL
LASATEAAAALGDGSCGTVFAARPQRMSARKFWVRYAAEATGSLTLDAGAVRAVVASRRSLLPAGITGVSGRFHGGDVVE
LRDADGTMVGRGVVAYDVAELATMLGRSTNELPEDLRRPAVHADDLVT

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=271, Percent_Identity=30.6273062730627, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI62912457, Length=269, Percent_Identity=30.4832713754647, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=39.7260273972603, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17569969, Length=280, Percent_Identity=31.4285714285714, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17569967, Length=280, Percent_Identity=31.4285714285714, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=402, Percent_Identity=32.089552238806, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321822, Length=355, Percent_Identity=32.3943661971831, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI21357643, Length=322, Percent_Identity=31.6770186335404, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 38039; Mature: 38039

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50890 PUA ; PS00435 PEROXIDASE_1 ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPGAQAREAIRSARTVVVKVGTAALTDETGTFSADRMEYLVEVIEGRMKAGSDVVIVSS
CCCCHHHHHHHHHHCEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEC
GAIAAGMVPLGLTKRPTDLATKQAAASAGQVALIGAWSAAFARYERTVGQVLLTAHDISM
CCHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEEE
RVHHTNAQRTLDRLTALHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGADALVLLSD
EEECCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCHHHHHHHHHHHCCCCEEEEHH
IDGLYDSDPRKGNARFISEVSGPEDLADVIAGPGGSRGTGGMASKLSSALLASDAGVPVL
CCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
LASATEAAAALGDGSCGTVFAARPQRMSARKFWVRYAAEATGSLTLDAGAVRAVVASRRS
EECCHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHH
LLPAGITGVSGRFHGGDVVELRDADGTMVGRGVVAYDVAELATMLGRSTNELPEDLRRPA
CCCCCCCCCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
VHADDLVT
CCCHHCCC
>Mature Secondary Structure
MNPGAQAREAIRSARTVVVKVGTAALTDETGTFSADRMEYLVEVIEGRMKAGSDVVIVSS
CCCCHHHHHHHHHHCEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEC
GAIAAGMVPLGLTKRPTDLATKQAAASAGQVALIGAWSAAFARYERTVGQVLLTAHDISM
CCHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEEE
RVHHTNAQRTLDRLTALHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGADALVLLSD
EEECCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCHHHHHHHHHHHCCCCEEEEHH
IDGLYDSDPRKGNARFISEVSGPEDLADVIAGPGGSRGTGGMASKLSSALLASDAGVPVL
CCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
LASATEAAAALGDGSCGTVFAARPQRMSARKFWVRYAAEATGSLTLDAGAVRAVVASRRS
EECCHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHH
LLPAGITGVSGRFHGGDVVELRDADGTMVGRGVVAYDVAELATMLGRSTNELPEDLRRPA
CCCCCCCCCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
VHADDLVT
CCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA