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The map label for this gene is folC [H]

Identifier: 169628694

GI number: 169628694

Start: 1634859

End: 1636295

Strand: Direct

Name: folC [H]

Synonym: MAB_1604

Alternate gene names: 169628694

Gene position: 1634859-1636295 (Clockwise)

Preceding gene: 169628693

Following gene: 169628695

Centisome position: 32.26

GC content: 65.34

Gene sequence:

>1437_bases
GTGAGTGAACCACTGAGTTCGGAGCCTTCACCCGACGAGATCGCGGCTCTGCTGCAGATCGAGTATCTACTCGACCAACG
CTGGCCCGAAACCAAGATCGAACCGTCAACCGATCGCATCGTGGCCCTCATGGAATTGCTGGGCTCGCCTCAGCGCGCGT
ATCCGTGCATCCATGTTGCGGGTACCAACGGCAAGACCTCGGTGACCAGGATGATCGACGCGCTCTTGACGGCACTGCAC
CGGCGCACCGGCCGCACCACCAGCCCGCATCTGCAGTCCGCGGTGGAGCGCATTGCCATTGACGGACAACCGATCTCGCC
AGCGAAGTACGTGGAGATCTACTCCGAGATCGAACCGTTCGTGGAGCTGGTGGACAAGCAGTCGCAGGAGCAGGGCGGAC
CCGCGATGAGCAAGTTCGAAGTGCTGGTCGCCATGGCTTTCGTGGCGTTCGCCGACGCGCCCGTCGATATCGCCGTGGTG
GAGGTCGGGCTTGGTGGGCGCTGGGATGCCACCAACATCATCGACGCCCCGGTCGCCGTCGTCACCCCCATAGGCATCGA
CCACGTCGAGTTCCTCGGACCAGACCTGGCCTCGATCGCGAATGAGAAGGCCGGAATTATCAAGCGGCACAAGCTTGACG
CGATGACCGGTGAAGCCGGCGCCGATACGGTCGCGGTGATCGCCCAGCAGCAGCCGGAGGCGATGGAAGTGCTGCTGCGT
CAAACGGTGGAAGCCGACGCGGCCGTCGCACGCGAGGGTTCGGAGTTCGCGGTGTTGTCCCGGCAGGTGGCGGTCGGCGG
CCAGCTCCTGGAATTGCAAGGTCTGGGCGGGGTGTACCCGGAGATCTTCCTGCCGCTGCACGGTGAGCATCAGGCACACA
ACGCGGCGGTGGCACTCGCGGCAGTCGAGGCGTTCTTCGGGGCGGGGGCCGATCGTCAGCTCGACATCGAGGCGATTCGC
TCGGGTTTCGCCTCGGTGATCATCCCGGGACGCTTGGAGCGGGTGCGTAGTGCTCCCGCGGTATTCCTGGACGCCGCCCA
CAATCCGCATGGGGCCGCCGCACTGGCCGAGACCCTGCAATCGGAGTTCGACTTCCGACGTCTGGTCGGCGTCATCAGCG
TGATGGGCGACAAGGATGTCGCGGGAATTCTGGGCGCACTGGAACCCGCATTCGACGAGATCGTGGTGACGCACAACGGA
TCTCCCCGCGCGATGGAGACGGACGCGCTGGCGGGTATCGCCCTGGAGATCTTCGGTGAGGATCGCGTCGTGGTGGCGCC
GACATTGCTCGATGCGGTCGAGACGGCCACCGCGATGGTGGAGGAGGCCGCCGAGGACGGGGGAGCGGAGGGCTTCTCCG
GCACCGGCATCGTGATCACCGGATCGGTCGTCACCGCGGGTGCCGCACGGACCCTGTTCGGAAAGGATCCGCAATGA

Upstream 100 bases:

>100_bases
TGGCCAAGGCCCCGGAGGCGGTCGTGGAGAAGATTCGGGTTCGCCAGCAGGTGGCTACCGAGGAAGTCGAGCGGATCACC
GCCCGTTTGGCGGCGCTGGC

Downstream 100 bases:

>100_bases
GTGATCCCGAGGACGGCATCGAACCCCGCGCGGACCAGAATGTCGCCGGCGCGGCTCCGCCAGATCCCTGGCGGAGTTTC
CGCGGCGTGATGGCGGGCAC

Product: folylpolyglutamate synthase FolC

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSEPLSSEPSPDEIAALLQIEYLLDQRWPETKIEPSTDRIVALMELLGSPQRAYPCIHVAGTNGKTSVTRMIDALLTALH
RRTGRTTSPHLQSAVERIAIDGQPISPAKYVEIYSEIEPFVELVDKQSQEQGGPAMSKFEVLVAMAFVAFADAPVDIAVV
EVGLGGRWDATNIIDAPVAVVTPIGIDHVEFLGPDLASIANEKAGIIKRHKLDAMTGEAGADTVAVIAQQQPEAMEVLLR
QTVEADAAVAREGSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAAVALAAVEAFFGAGADRQLDIEAIR
SGFASVIIPGRLERVRSAPAVFLDAAHNPHGAAALAETLQSEFDFRRLVGVISVMGDKDVAGILGALEPAFDEIVVTHNG
SPRAMETDALAGIALEIFGEDRVVVAPTLLDAVETATAMVEEAAEDGGAEGFSGTGIVITGSVVTAGAARTLFGKDPQ

Sequences:

>Translated_478_residues
MSEPLSSEPSPDEIAALLQIEYLLDQRWPETKIEPSTDRIVALMELLGSPQRAYPCIHVAGTNGKTSVTRMIDALLTALH
RRTGRTTSPHLQSAVERIAIDGQPISPAKYVEIYSEIEPFVELVDKQSQEQGGPAMSKFEVLVAMAFVAFADAPVDIAVV
EVGLGGRWDATNIIDAPVAVVTPIGIDHVEFLGPDLASIANEKAGIIKRHKLDAMTGEAGADTVAVIAQQQPEAMEVLLR
QTVEADAAVAREGSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAAVALAAVEAFFGAGADRQLDIEAIR
SGFASVIIPGRLERVRSAPAVFLDAAHNPHGAAALAETLQSEFDFRRLVGVISVMGDKDVAGILGALEPAFDEIVVTHNG
SPRAMETDALAGIALEIFGEDRVVVAPTLLDAVETATAMVEEAAEDGGAEGFSGTGIVITGSVVTAGAARTLFGKDPQ
>Mature_477_residues
SEPLSSEPSPDEIAALLQIEYLLDQRWPETKIEPSTDRIVALMELLGSPQRAYPCIHVAGTNGKTSVTRMIDALLTALHR
RTGRTTSPHLQSAVERIAIDGQPISPAKYVEIYSEIEPFVELVDKQSQEQGGPAMSKFEVLVAMAFVAFADAPVDIAVVE
VGLGGRWDATNIIDAPVAVVTPIGIDHVEFLGPDLASIANEKAGIIKRHKLDAMTGEAGADTVAVIAQQQPEAMEVLLRQ
TVEADAAVAREGSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAAVALAAVEAFFGAGADRQLDIEAIRS
GFASVIIPGRLERVRSAPAVFLDAAHNPHGAAALAETLQSEFDFRRLVGVISVMGDKDVAGILGALEPAFDEIVVTHNGS
PRAMETDALAGIALEIFGEDRVVVAPTLLDAVETATAMVEEAAEDGGAEGFSGTGIVITGSVVTAGAARTLFGKDPQ

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=379, Percent_Identity=31.6622691292876, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI66932984, Length=379, Percent_Identity=31.6622691292876, Blast_Score=139, Evalue=6e-33,
Organism=Escherichia coli, GI1788654, Length=417, Percent_Identity=32.8537170263789, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17553148, Length=320, Percent_Identity=30.625, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17553150, Length=320, Percent_Identity=30.625, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71984923, Length=320, Percent_Identity=30.625, Blast_Score=108, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6323760, Length=388, Percent_Identity=29.639175257732, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6324815, Length=160, Percent_Identity=36.25, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322718, Length=406, Percent_Identity=26.1083743842365, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24641571, Length=194, Percent_Identity=37.6288659793814, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581568, Length=180, Percent_Identity=33.8888888888889, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 50332; Mature: 50200

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLSSEPSPDEIAALLQIEYLLDQRWPETKIEPSTDRIVALMELLGSPQRAYPCIHVA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEE
GTNGKTSVTRMIDALLTALHRRTGRTTSPHLQSAVERIAIDGQPISPAKYVEIYSEIEPF
CCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VELVDKQSQEQGGPAMSKFEVLVAMAFVAFADAPVDIAVVEVGLGGRWDATNIIDAPVAV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHH
VTPIGIDHVEFLGPDLASIANEKAGIIKRHKLDAMTGEAGADTVAVIAQQQPEAMEVLLR
EECCCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
QTVEADAAVAREGSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAAVALA
HHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHH
AVEAFFGAGADRQLDIEAIRSGFASVIIPGRLERVRSAPAVFLDAAHNPHGAAALAETLQ
HHHHHHCCCCCCEEEHHHHHCCHHEEECCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
SEFDFRRLVGVISVMGDKDVAGILGALEPAFDEIVVTHNGSPRAMETDALAGIALEIFGE
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHEEEEEEECC
DRVVVAPTLLDAVETATAMVEEAAEDGGAEGFSGTGIVITGSVVTAGAARTLFGKDPQ
CCEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEECCCCHHHCCCCCCC
>Mature Secondary Structure 
SEPLSSEPSPDEIAALLQIEYLLDQRWPETKIEPSTDRIVALMELLGSPQRAYPCIHVA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEE
GTNGKTSVTRMIDALLTALHRRTGRTTSPHLQSAVERIAIDGQPISPAKYVEIYSEIEPF
CCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VELVDKQSQEQGGPAMSKFEVLVAMAFVAFADAPVDIAVVEVGLGGRWDATNIIDAPVAV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHH
VTPIGIDHVEFLGPDLASIANEKAGIIKRHKLDAMTGEAGADTVAVIAQQQPEAMEVLLR
EECCCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
QTVEADAAVAREGSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAAVALA
HHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHH
AVEAFFGAGADRQLDIEAIRSGFASVIIPGRLERVRSAPAVFLDAAHNPHGAAALAETLQ
HHHHHHCCCCCCEEEHHHHHCCHHEEECCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
SEFDFRRLVGVISVMGDKDVAGILGALEPAFDEIVVTHNGSPRAMETDALAGIALEIFGE
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHEEEEEEECC
DRVVVAPTLLDAVETATAMVEEAAEDGGAEGFSGTGIVITGSVVTAGAARTLFGKDPQ
CCEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEECCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]