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The map label for this gene is pinE [H]

Identifier: 16766015

GI number: 16766015

Start: 2851384

End: 2851941

Strand: Reverse

Name: pinE [H]

Synonym: STM2702

Alternate gene names: 16766015

Gene position: 2851941-2851384 (Counterclockwise)

Preceding gene: 16766018

Following gene: 16766014

Centisome position: 58.71

GC content: 50.36

Gene sequence:

>558_bases
GTGCAAATTGGCTATGTACGGGTGTCAACAAATGACCAGAATACTGATTTACAGCGAAACGCGCTGAATTGCGCAGGATG
TGAACTGATTTTTGAAGACAAAATAAGCGGGACCAAATCAGAAAGGCCCGGATTAAAAAAGTTGCTCAGGACGTTATCAG
AAGGGGACACACTGGTTGTATGGAAGCTTGATCGCCTGGGTCGCAGTATGCGTCATCTGGTCGTTCTGGTAGAGGAGCTG
CGGGAGCGTGGCATTAATTTCCGCAGTCTTACGGATAGCATAGATACTTCCACATCAATGGGGCGTTTCTTTTTCCATAT
CATGGGCGCACTCGCTGAAATGGAGAGGGAATTAATTGTGGAAAGAACCCGCGCCGGACTTGCCGCTGCGAGGGCGCAAG
GGCGAGTCGGTGGCAGGCGTCCAAAGCTCACTCCGGAGCAATGGGAGCAGGCCGGACGGTTGCTTGCCGCCGGTGAAACT
CGTCATCGTGTTGGATTGCTTTTTGATGTTAGCATTTCCACTCTTTACAAGAAATTCCCTGTAAATCAGTCGCGTTGA

Upstream 100 bases:

>100_bases
CCCGCAGCTTGCTTTGCTGTTTCGCCTAAACCGAGGTTTATGAAAATGCAGAAAACGGTCGTAAATGGCATGATCCCAGC
CTTTTGCGAGGGGGCCTGTT

Downstream 100 bases:

>100_bases
AAGTGGCGATATTGTACCAGCACTGACACATCGTGAAATACGTGCGCCGCACGCCTGGCAACCAGAACATAAGGTATCCC
TGTCAACCGGAGAGACTGCC

Product: DNA invertase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MQIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSEGDTLVVWKLDRLGRSMRHLVVLVEEL
RERGINFRSLTDSIDTSTSMGRFFFHIMGALAEMERELIVERTRAGLAAARAQGRVGGRRPKLTPEQWEQAGRLLAAGET
RHRVGLLFDVSISTLYKKFPVNQSR

Sequences:

>Translated_185_residues
MQIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSEGDTLVVWKLDRLGRSMRHLVVLVEEL
RERGINFRSLTDSIDTSTSMGRFFFHIMGALAEMERELIVERTRAGLAAARAQGRVGGRRPKLTPEQWEQAGRLLAAGET
RHRVGLLFDVSISTLYKKFPVNQSR
>Mature_185_residues
MQIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSEGDTLVVWKLDRLGRSMRHLVVLVEEL
RERGINFRSLTDSIDTSTSMGRFFFHIMGALAEMERELIVERTRAGLAAARAQGRVGGRRPKLTPEQWEQAGRLLAAGET
RHRVGLLFDVSISTLYKKFPVNQSR

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=180, Percent_Identity=88.3333333333333, Blast_Score=334, Evalue=2e-93,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=37.7049180327869, Blast_Score=106, Evalue=7e-25,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=37.7049180327869, Blast_Score=105, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20902; Mature: 20902

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSEGDTLVV
CCEEEEEEECCCCCCCHHHHCCCHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVVLVEELRERGINFRSLTDSIDTSTSMGRFFFHIMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ERTRAGLAAARAQGRVGGRRPKLTPEQWEQAGRLLAAGETRHRVGLLFDVSISTLYKKFP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEECCCCCCEEEEEEEHHHHHHHHHCC
VNQSR
CCCCC
>Mature Secondary Structure
MQIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSEGDTLVV
CCEEEEEEECCCCCCCHHHHCCCHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVVLVEELRERGINFRSLTDSIDTSTSMGRFFFHIMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ERTRAGLAAARAQGRVGGRRPKLTPEQWEQAGRLLAAGETRHRVGLLFDVSISTLYKKFP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEECCCCCCEEEEEEEHHHHHHHHHCC
VNQSR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]