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The map label for this gene is phrB

Identifier: 16764079

GI number: 16764079

Start: 774778

End: 776199

Strand: Direct

Name: phrB

Synonym: STM0709

Alternate gene names: 16764079

Gene position: 774778-776199 (Clockwise)

Preceding gene: 16764078

Following gene: 16764081

Centisome position: 15.95

GC content: 56.96

Gene sequence:

>1422_bases
ATGCCCACCCATTTAGTCTGGTTCAGGCGCGATCTGCGTTTACAGGATAACCTTGCGCTGGCCGCCGCCTGCCGCGATGC
ATCCGCGCGGGTGCTGGCGCTTTATATTTCCACCCCCGCGCAGTGGCAGGCCCATGATATGGCGCCGCGGCAGGCGGCAT
TCATCAGCGCGCAGCTCAATGCGCTACAGACGGCCCTTGCAGAGAAAGGCATTCCGTTGCTGTTTCATGAAGTGGCGGAT
TTTAACGCCAGTATTGAAACGGTCAAAAACGTTTGCCGACAGCATGACGTCAGCCATCTGTTTTATAACTATCAGTATGA
GTTTAACGAGCGTCAGCGTGATGCGGCGGTGGAAAAAACGCTGCCCTCTGTCATCTGCGAAGGCTTTGACGATAGCGTGA
TTCTGGCGCCCGGCGCGGTGATGACCGGCAATCATGAAATGTATAAAGTTTTTACGCCGTTTAAAAATGCCTGGTTGAAA
CGGCTAAAAGAAGATATCCCGCCGTGCGTTCCGGCCCCGAAGATCCGGGTGAGCGGCGCGCTTTCTACACCCTTAACGCC
AGTCTCGCTTAACTACCCGCAACAGGCGTTTGACGCCGCGCTTTTCCCGGTGGAAGAAAACGCGGTCATCGCGCAGCTAC
GTCAGTTTTGCGCGCAGGGCGCAGACGAGTATGCGTTACGGCGCGATTTCCCTGCGGTTGACGGCACCAGTCGGCTTTCC
GCCAGCCTGGCGACGGGCGGCCTGTCGCCGCGACAGTGCCTGCACCGATTACTGGCGGAGCAGCCGCAGGCGCTGGACGG
CGGGCCGGGAAGCGTCTGGTTGAATGAGCTCATCTGGCGGGAATTTTACCGTCATTTAATGACCTGGTATCCGGCATTAT
GCAAACACCAGCCGTTTATCCGCTGGACAAAACGCGTCGCGTGGCAGGAAAACCCGCACTATTTTCAGGCATGGCAGAAA
GGCGAAACCGGTTATCCCATTGTCGATGCGGCGATGCGCCAGCTTAACGCGACGGGCTGGATGCATAACCGTTTACGCAT
GATTACCGCCAGCTTTCTGGTAAAAGATCTGTTGATTGACTGGCGGTTGGGGGAGCGCTATTTCATGTCGCAGCTTATTG
ACGGCGATCTTGCCGCCAATAATGGCGGCTGGCAGTGGGCCGCCTCAACCGGTACTGACGCCGCGCCTTATTTTCGTATT
TTTAATCCCACGACTCAGGGAGAGAGGTTCGATCGCGACGGCGAATTTATCCGCCAGTGGCTACCGGCATTACGCGATAT
CCCTGGAAAAGCGATTCACGAGCCGTGGCGGTGGGCGGAAAAAGCGGGAGTCGTGCTTGATTATCCTCGGCCTATTGTGG
AGCACAAACAGGCGAGAATCGCAACGCTTTCCGCCTATGAGGCGGCGAGAAAAGGGGCGTGA

Upstream 100 bases:

>100_bases
CCTGAAAAACCGTCAGCCGCCACGCTGGCGGTTTTTTTATGCGCCAGGGTCTACACTGTTTAACGCTATTCGCCGTGACG
TTTCATCTTTCAGGAGTTGT

Downstream 100 bases:

>100_bases
TACAGGCCCGGTAGCGCAAACTACCGGGCATTCTGCTATCAGGATTCGACGGCCAGCGCGCGGCTTTTGAATTTGAAGGA
CTGATACAGCCAAATCAGCA

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MPTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLNALQTALAEKGIPLLFHEVAD
FNASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKTLPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLK
RLKEDIPPCVPAPKIRVSGALSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYALRRDFPAVDGTSRLS
ASLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFIRWTKRVAWQENPHYFQAWQK
GETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLIDWRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRI
FNPTTQGERFDRDGEFIRQWLPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA

Sequences:

>Translated_473_residues
MPTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLNALQTALAEKGIPLLFHEVAD
FNASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKTLPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLK
RLKEDIPPCVPAPKIRVSGALSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYALRRDFPAVDGTSRLS
ASLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFIRWTKRVAWQENPHYFQAWQK
GETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLIDWRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRI
FNPTTQGERFDRDGEFIRQWLPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA
>Mature_472_residues
PTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLNALQTALAEKGIPLLFHEVADF
NASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKTLPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLKR
LKEDIPPCVPAPKIRVSGALSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYALRRDFPAVDGTSRLSA
SLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFIRWTKRVAWQENPHYFQAWQKG
ETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLIDWRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIF
NPTTQGERFDRDGEFIRQWLPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain

Homologues:

Organism=Homo sapiens, GI188536100, Length=498, Percent_Identity=26.9076305220884, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4758072, Length=492, Percent_Identity=25.2032520325203, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI188536103, Length=286, Percent_Identity=30.7692307692308, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1786926, Length=472, Percent_Identity=79.2372881355932, Blast_Score=801, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324962, Length=506, Percent_Identity=33.201581027668, Blast_Score=259, Evalue=6e-70,
Organism=Drosophila melanogaster, GI17137248, Length=505, Percent_Identity=28.1188118811881, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24585455, Length=505, Percent_Identity=28.1188118811881, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24648152, Length=262, Percent_Identity=29.7709923664122, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHR_SALTY (P25078)

Other databases:

- EMBL:   X60662
- EMBL:   AE006468
- PIR:   S22321
- RefSeq:   NP_459694.1
- ProteinModelPortal:   P25078
- SMR:   P25078
- GeneID:   1252229
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM0709
- NMPDR:   fig|99287.1.peg.682
- HOGENOM:   HBG693486
- OMA:   ADASPYF
- ProtClustDB:   PRK10674
- BioCyc:   STYP99287:STM0709-MONOMER
- BRENDA:   4.1.99.3
- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00147

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7; SSF52425 DNA_photolyase_N; SSF48173 Photolyase_FAD-bd/Cryptochr_C

EC number: =4.1.99.3

Molecular weight: Translated: 53676; Mature: 53545

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1; PS00691 DNA_PHOTOLYASES_1_2

Important sites: BINDING 224-224 BINDING 228-228 BINDING 406-406

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLN
CCCCEEEHHHCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHHHH
ALQTALAEKGIPLLFHEVADFNASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKT
HHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCHHHEEEEEEECCHHHHHHHHHHH
LPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLKRLKEDIPPCVPAPKIRVSGA
HHHHHHCCCCCCEEECCCEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCEEECCC
LSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYALRRDFPAVDGTSRLS
CCCCCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHH
ASLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFI
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RWTKRVAWQENPHYFQAWQKGETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLID
HHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
WRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGERFDRDGEFIRQW
HHCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
LPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA
HHHHHCCCCHHHCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTHLVWFRRDLRLQDNLALAAACRDASARVLALYISTPAQWQAHDMAPRQAAFISAQLN
CCCEEEHHHCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHHHH
ALQTALAEKGIPLLFHEVADFNASIETVKNVCRQHDVSHLFYNYQYEFNERQRDAAVEKT
HHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCHHHEEEEEEECCHHHHHHHHHHH
LPSVICEGFDDSVILAPGAVMTGNHEMYKVFTPFKNAWLKRLKEDIPPCVPAPKIRVSGA
HHHHHHCCCCCCEEECCCEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCEEECCC
LSTPLTPVSLNYPQQAFDAALFPVEENAVIAQLRQFCAQGADEYALRRDFPAVDGTSRLS
CCCCCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHH
ASLATGGLSPRQCLHRLLAEQPQALDGGPGSVWLNELIWREFYRHLMTWYPALCKHQPFI
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RWTKRVAWQENPHYFQAWQKGETGYPIVDAAMRQLNATGWMHNRLRMITASFLVKDLLID
HHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
WRLGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGERFDRDGEFIRQW
HHCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
LPALRDIPGKAIHEPWRWAEKAGVVLDYPRPIVEHKQARIATLSAYEAARKGA
HHHHHCCCCHHHCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840665; 11677609