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The map label for this gene is gltK [H]

Identifier: 16764040

GI number: 16764040

Start: 727335

End: 728009

Strand: Reverse

Name: gltK [H]

Synonym: STM0663

Alternate gene names: 16764040

Gene position: 728009-727335 (Counterclockwise)

Preceding gene: 16764041

Following gene: 16764039

Centisome position: 14.99

GC content: 53.78

Gene sequence:

>675_bases
ATGTACGAATTTGACTGGAGTTCGATTATCCCCTCACTGCCTTACCTACTGGCCGGGTTGGTCATCACGCTGAAGATCAC
CGTCACCGCCGTTATCGTGGGGATAGTCTGGGGAACCATTCTGGCCGTCATGCGCCTCTCAAGCTTTGCCCCTATCGCCT
GGTTTGCTAAAGCGTATGTTAACGTTTTCCGTTCCATCCCGTTGGTGATGGTCTTGCTGTGGTTCTATCTGATTGTACCC
GGCTTTCTGCAAAACGTGCTGGGACTATCGCCGAAAACCGATATCCGCCTGATCTCCGCGATGGTGGCATTCTCCATGTT
TGAAGCGGCCTACTATTCCGAGATTATTCGCGCCGGTATTCAAAGTATTTCCCGCGGGCAGTCCAGCGCCGCGCTGGCGC
TGGGGATGACCCACTGGCAGTCGATGAAGCTCATTATTCTGCCGCAGGCGTTTCGGGCGATGGTGCCGCTGTTGCTTACG
CAGGGCATCGTGCTATTTCAGGACACCTCTTTAGTTTATGTACTCAGCCTGGCGGACTTCTTCCGCACCGCGTCGACTAT
CGGCGAGCGTGACGGTACTCAGGTCGAAATGATTCTGTTCGCCGGCGCCGTTTATTTTGTTATCAGCCTGAGCGCGTCGT
TGCTGGTCAGCTATTTGAAGAAAAGGACTGTTTAA

Upstream 100 bases:

>100_bases
CCATTACGCTGGCCTATGTGCTGATTAACGCCGTTATCATGCTGGTCATGAGCCTGGTTGAACGCAAAGTCCGCCTGCCG
GGCAACGTGGGGAGCAAATA

Downstream 100 bases:

>100_bases
TGATTACCCTGAAAAATGTTTCAAAATGGTATGGTCACTTTCAGGTGCTGACCGATTGTTCAACAGAAGTGAAAAAAGGT
GAGGTGGTAGTAGTCTGCGG

Product: glutamate/aspartate transporter

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MYEFDWSSIIPSLPYLLAGLVITLKITVTAVIVGIVWGTILAVMRLSSFAPIAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKTDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGAVYFVISLSASLLVSYLKKRTV

Sequences:

>Translated_224_residues
MYEFDWSSIIPSLPYLLAGLVITLKITVTAVIVGIVWGTILAVMRLSSFAPIAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKTDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGAVYFVISLSASLLVSYLKKRTV
>Mature_224_residues
MYEFDWSSIIPSLPYLLAGLVITLKITVTAVIVGIVWGTILAVMRLSSFAPIAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKTDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGAVYFVISLSASLLVSYLKKRTV

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=223, Percent_Identity=95.5156950672646, Blast_Score=426, Evalue=1e-121,
Organism=Escherichia coli, GI1788226, Length=214, Percent_Identity=37.3831775700935, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI87082239, Length=208, Percent_Identity=31.7307692307692, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1787030, Length=224, Percent_Identity=31.6964285714286, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1786874, Length=172, Percent_Identity=28.4883720930233, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787087, Length=217, Percent_Identity=28.110599078341, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994930, Length=122, Percent_Identity=34.4262295081967, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788646, Length=211, Percent_Identity=24.1706161137441, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788645, Length=211, Percent_Identity=26.0663507109005, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787086, Length=216, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24778; Mature: 24778

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEFDWSSIIPSLPYLLAGLVITLKITVTAVIVGIVWGTILAVMRLSSFAPIAWFAKAYV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKTDIRLISAMVAFSMFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF
HHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
FRTASTIGERDGTQVEMILFAGAVYFVISLSASLLVSYLKKRTV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYEFDWSSIIPSLPYLLAGLVITLKITVTAVIVGIVWGTILAVMRLSSFAPIAWFAKAYV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKTDIRLISAMVAFSMFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF
HHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
FRTASTIGERDGTQVEMILFAGAVYFVISLSASLLVSYLKKRTV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]