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The map label for this gene is entE [H]

Identifier: 16763973

GI number: 16763973

Start: 657440

End: 659050

Strand: Direct

Name: entE [H]

Synonym: STM0596

Alternate gene names: 16763973

Gene position: 657440-659050 (Clockwise)

Preceding gene: 16763972

Following gene: 16763974

Centisome position: 13.53

GC content: 57.42

Gene sequence:

>1611_bases
ATGCGTATACCTTTCACCCGTTGGCCGGATGAATTTGCCCGCCGTTATCGTGAAAAAGGCTACTGGCAAGACGTGCCGTT
GACCGATATTCTGACCCGCCACGCTGACAGCGACAAGACGGCGGTCATTGAAGGCGAGCGCGCATTCAGCTATCGCCAGC
TCAACCAGGCCGCAGATAATCTGGCCTGCAGTTTACGCCGTCAGGGCATCAAACCTGGCGAAACCGCTCTGGTACAACTG
GGGAATGTACCGGAACTGTATATCACCTTTTTCGCCCTGTTGAAGCTTGGCGTAGCGCCCGTTCTGGCGCTGTTTAGCCA
TCAACGTACCGAACTTAACGCCTATGCGATGCAGATCGCGCCGACGCTGGTGATTGCCGATCGCCAACATACGCTGTTCG
CCGGGGAGGACTTTCTCAACACGTTTGTGGCTGAACACCGCTCTGTGCGGGTGGTGTTATTGCGCAACGACGACGGCGAT
CACAGCCTGGACGCGGCGATGCGGCAGGCGGCGGAAGATTTCACTGCCACGCCATCACCTGCTGACGAAGTGGCCTACTT
TCAGCTTTCCGGCGGTACTACCGGCACGCCAAAGCTTATTCCCCGTACCCATAACGACTATTACTACAGCGTGCGCCGCA
GCAATGAGATTTGCGGTTTCAACGAGGATACGCGTTTTCTGTGCGCGATTCCCGCCGCGCATAACTACGCCATGAGTTCG
CCGGGCGCGCTGGGCGTCTTTCTTGCCAAAGGAACGGTAGTGCTGGCGACCGATCCCAGCGCCACGCTCTGTTTCCCGCT
GATCGAAAAACACCAGATTAATGCCACAGCGCTGGTGCCGCCCGCGGTCAGTCTATGGCTACAGGCTATCCAGGAGTGGG
GCGGCAATGCGCCGCTGGCGTCATTAAGGTTATTGCAGGTCGGCGGCGCGCGGCTTTCTGCGACGCTGGCCGCCCGTATT
CCGGCTGAAATTGGCTGTCAGTTGCAGCAGGTCTTCGGTATGGCGGAAGGGTTAGTGAACTATACCCGGCTGGACGATAG
TCCGGAACGGATTATCAATACCCAGGGAAGACCCATGTGTCCGGACGACGAAGTGTGGGTGGCGGATGCCGACGGGAATC
CACTGCCGCCGGGCGAGATTGGTCGTCTGATGACGCGCGGCCCCTATACTTTTCGCGGCTATTTCAACAGTCCGCTACAC
AATGCCAGCGCCTTTGACGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCCATTGATCAAGACGGTTACATCACCGT
TCACGGGCGTGAAAAAGATCAGATCAATCGGGGCGGCGAGAAGATAGCCGCCGAAGAGATAGAAAACCTGTTACTGCGCC
ACCCGGTGGTGATCCATGCGGCGCTGGTCAGCATGGAAGATGAACTGCTGGGAGAAAAAAGTTGCGCATATCTGGTGGTA
AAAGAGCCGCTGCGAGCGGTACAGGTACGCCGTTTCCTGCGAGAGCAGGGCGTGGCGGAATTTAAATTACCGGATCGCGT
GGAGTGCGTTGCGTCACTGCCGCTGACGCCGGTTGGTAAAGTCGATAAAAAACAATTACGCCAGCGGTTGGCGTCACGTT
CACCGCTCTGA

Upstream 100 bases:

>100_bases
CGGGATTGTCCCTGCCTCTTCGCCAGTCGGCGAGTGGCGGGAAACCGGCGTCAAGCTCTCTACGATGTTGAACGTTTTTG
GACTGCATTAAGGAACGATG

Downstream 100 bases:

>100_bases
AGGAGAAAGAGAGATGGCAATCCCGAAACTACAGTCTTACGCGCTGCCCACCGCACTGGATATCCCGACCAACAAAGTGA
ACTGGGCATTTGAGCCGGAG

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADNLACSLRRQGIKPGETALVQL
GNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGD
HSLDAAMRQAAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEDTRFLCAIPAAHNYAMSS
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPLH
NASAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPVVIHAALVSMEDELLGEKSCAYLVV
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL

Sequences:

>Translated_536_residues
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADNLACSLRRQGIKPGETALVQL
GNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGD
HSLDAAMRQAAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEDTRFLCAIPAAHNYAMSS
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPLH
NASAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPVVIHAALVSMEDELLGEKSCAYLVV
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
>Mature_536_residues
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADNLACSLRRQGIKPGETALVQL
GNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGD
HSLDAAMRQAAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEDTRFLCAIPAAHNYAMSS
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPLH
NASAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPVVIHAALVSMEDELLGEKSCAYLVV
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=24.8031496062992, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI122937307, Length=513, Percent_Identity=24.3664717348928, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI42544132, Length=496, Percent_Identity=23.7903225806452, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI58082049, Length=561, Percent_Identity=23.7076648841355, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI157311624, Length=563, Percent_Identity=23.2682060390764, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI157311622, Length=563, Percent_Identity=23.2682060390764, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI156151445, Length=192, Percent_Identity=30.7291666666667, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=22.6120857699805, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI187761345, Length=445, Percent_Identity=23.8202247191011, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI187761343, Length=445, Percent_Identity=23.8202247191011, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI28416953, Length=527, Percent_Identity=23.3396584440228, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI8923896, Length=571, Percent_Identity=20.3152364273205, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI253970489, Length=585, Percent_Identity=19.4871794871795, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=86.3295880149813, Blast_Score=958, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=32.5842696629214, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI221142682, Length=488, Percent_Identity=26.4344262295082, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788107, Length=532, Percent_Identity=24.0601503759398, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1786529, Length=540, Percent_Identity=23.3333333333333, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790505, Length=549, Percent_Identity=23.6794171220401, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789201, Length=367, Percent_Identity=23.1607629427793, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786801, Length=527, Percent_Identity=22.7703984819734, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=544, Percent_Identity=26.1029411764706, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=540, Percent_Identity=27.037037037037, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=532, Percent_Identity=25.187969924812, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994694, Length=532, Percent_Identity=25.187969924812, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=532, Percent_Identity=25.187969924812, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=525, Percent_Identity=24.5714285714286, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=528, Percent_Identity=24.8106060606061, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=23.6111111111111, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=23.2741617357002, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=545, Percent_Identity=21.8348623853211, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=351, Percent_Identity=27.3504273504274, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=525, Percent_Identity=23.4285714285714, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=534, Percent_Identity=22.0973782771536, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=534, Percent_Identity=22.0973782771536, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560140, Length=504, Percent_Identity=23.2142857142857, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=26.5193370165746, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=570, Percent_Identity=22.4561403508772, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24581924, Length=535, Percent_Identity=25.4205607476636, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922652, Length=493, Percent_Identity=23.5294117647059, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI18859661, Length=587, Percent_Identity=22.3168654173765, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355181, Length=525, Percent_Identity=22.8571428571429, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24656500, Length=499, Percent_Identity=21.8436873747495, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356947, Length=436, Percent_Identity=23.8532110091743, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648255, Length=470, Percent_Identity=22.5531914893617, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648253, Length=476, Percent_Identity=22.4789915966387, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21356441, Length=494, Percent_Identity=22.0647773279352, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472339, Length=542, Percent_Identity=20.8487084870849, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667955, Length=542, Percent_Identity=20.8487084870849, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648260, Length=361, Percent_Identity=25.207756232687, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358303, Length=334, Percent_Identity=22.7544910179641, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648257, Length=385, Percent_Identity=24.1558441558442, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281365686, Length=325, Percent_Identity=24.6153846153846, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24667959, Length=127, Percent_Identity=31.496062992126, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 59256; Mature: 59256

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADN
CCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIA
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEC
PTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGDHSLDAAMRQAAEDFTATPSP
CEEEEECCCCEEECCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEDTRFLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCHH
SLRLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMC
HEEEEHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
PDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPLHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEECCCEEEEC
QDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPVVIHAALVSMEDELLGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEE
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADN
CCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIA
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEC
PTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGDHSLDAAMRQAAEDFTATPSP
CEEEEECCCCEEECCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEDTRFLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCHH
SLRLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMC
HEEEEHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
PDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPLHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEECCCEEEEC
QDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPVVIHAALVSMEDELLGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEE
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]