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The map label for this gene is tuf1 [H]

Identifier: 167626053

GI number: 167626053

Start: 5042228

End: 5043412

Strand: Reverse

Name: tuf1 [H]

Synonym: Shal_4148

Alternate gene names: 167626053

Gene position: 5043412-5042228 (Counterclockwise)

Preceding gene: 167626055

Following gene: 167626052

Centisome position: 96.49

GC content: 46.41

Gene sequence:

>1185_bases
ATGGCTAAAGAAAAATTTGAACGCGTAAAACCACACGTAAACGTTGGTACCATTGGACACGTTGACCATGGTAAAACTAC
TCTAACTGCTGCTATTTCTTCGGTATTAACGAAGACTTACGGTGGTACTAAGCGTGATTTCGCACAGATCGATAACGCTC
CAGAAGAGCGTGAGCGCGGTATTACAATTAATACTTCTCACATCGAGTATGATACTCCAGCACGTCACTACGCACACGTA
GACTGTCCAGGTCACGCGGATTATGTTAAAAACATGATCACTGGTGCTGCACAGATGGACGGCGCTATCCTAGTAGTAGC
TTCTACAGATGGTCCAATGCCACAGACTCGTGAGCACATCCTGCTTTCACGTCAGGTTGGCGTACCATTCATCATCGTAT
TCATGAACAAGTGTGACATGGTTGATGACGAAGAGCTTCTAGAACTAGTAGAAATGGAAGTTCGTGAACTTCTATCTGAA
TATGACTTCCCAGGTGATGACCTACCAGTTATCCAAGGTTCTGCGCTTAAAGCCCTTGAAGGCGAGCCAGAGTGGGAAGC
TAAGATCCTTGAGCTTGCAGAAGCACTAGATACTTATATTCCAGAGCCAGAGCGTGCTATCGATGGTGCATTCATTCTTC
CAATCGAAGATGTTTTCTCAATCGCTGGTCGTGGTACTGTTGTTACTGGTCGTGTCGAGCGCGGTATCGTCCGTGTTGGC
GACGAAGTAGAAATCGTTGGTATCAAAGATACAACTAAGTCTACTTGTACTGGTGTTGAAATGTTCCGTAAGCTTCTTGA
CGAAGGTCGTGCTGGCGAGAACTGTGGTGTTCTTCTACGTGGTATCAAGCGTGAAGATGTTGAGCGTGGTCAGGTTCTTG
CTGCTCCTGGTTCAATCACTCCACACACAACTTTCAAGTCAGAAATCTATGTTCTGTCTAAAGAAGAAGGCGGACGTCAC
ACTCCATTCTTCAAAGGCTACCGTCCACAGTTCTACTTCCGTACAACTGACGTAACCGGTACTATCGAATTGCCAGAAGG
CGTAGAGATGGTAATGCCTGGTGACAACGTAGCTATGACAGTAACTCTTATTGCTCCAATCGCGATGGACGAAGGTTTAC
GCTTCGCTATCCGTGAAGGTGGCCGTACAGTTGGTGCTGGTGTTGTAGCTGAGATCGTTGCTTAA

Upstream 100 bases:

>100_bases
TTCTGATTATCTTACTAACTGATTCGATGACATTTTCTATGCATCGGATTTTTTGCTATATGCAGTTTCATCACCAAATA
CTTTGGATTGGGGCAATACC

Downstream 100 bases:

>100_bases
TTAGCAATTGTCTTAACTCGATACTGAAAAAGGCAGCTTAGGCTGCCTTTTTTGTTTTTATTGCATTTAGGTTTAATTTG
TTAGTGAAGCTGGGCTATGT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERGITINTSHIEYDTPARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIVRVG
DEVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSITPHTTFKSEIYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMTVTLIAPIAMDEGLRFAIREGGRTVGAGVVAEIVA

Sequences:

>Translated_394_residues
MAKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERGITINTSHIEYDTPARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIVRVG
DEVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSITPHTTFKSEIYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMTVTLIAPIAMDEGLRFAIREGGRTVGAGVVAEIVA
>Mature_393_residues
AKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERGITINTSHIEYDTPARHYAHVD
CPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEY
DFPGDDLPVIQGSALKALEGEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIVRVGD
EVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSITPHTTFKSEIYVLSKEEGGRHT
PFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMTVTLIAPIAMDEGLRFAIREGGRTVGAGVVAEIVA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=54.9367088607595, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=29.1571753986333, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=29.1571753986333, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=28.8590604026846, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=28.1879194630872, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=29.5652173913043, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194018522, Length=445, Percent_Identity=27.6404494382022, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI194018520, Length=441, Percent_Identity=27.6643990929705, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI194097354, Length=441, Percent_Identity=27.6643990929705, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI46094014, Length=448, Percent_Identity=27.4553571428571, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI4503507, Length=272, Percent_Identity=27.2058823529412, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI53729339, Length=233, Percent_Identity=30.0429184549356, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=233, Percent_Identity=30.0429184549356, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=285, Percent_Identity=27.3684210526316, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=85.7868020304569, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=85.7868020304569, Blast_Score=678, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=30.2387267904509, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=29.7619047619048, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789108, Length=431, Percent_Identity=26.9141531322506, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788922, Length=328, Percent_Identity=26.219512195122, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.3809523809524, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=45.7142857142857, Blast_Score=347, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=456, Percent_Identity=28.9473684210526, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=456, Percent_Identity=28.9473684210526, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=442, Percent_Identity=27.3755656108597, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=433, Percent_Identity=27.7136258660508, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=27.6744186046512, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=29.1512915129151, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=24.6119733924612, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=159, Percent_Identity=35.8490566037736, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=28.3154121863799, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=28.0851063829787, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=62.2784810126582, Blast_Score=503, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=29.1479820627803, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=29.1479820627803, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=457, Percent_Identity=23.8512035010941, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=29.1907514450867, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=279, Percent_Identity=26.8817204301075, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=236, Percent_Identity=29.6610169491525, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=55.012853470437, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=55.012853470437, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50.7772020725389, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=27.4122807017544, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=27.4122807017544, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=27.4122807017544, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=27.4122807017544, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=27.6315789473684, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=27.6315789473684, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=30.1324503311258, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=335, Percent_Identity=29.5522388059701, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=27.6255707762557, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647048, Length=266, Percent_Identity=27.8195488721804, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647046, Length=266, Percent_Identity=27.8195488721804, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572034, Length=234, Percent_Identity=31.6239316239316, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43112; Mature: 42981

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERG
CCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHCC
ITINTSHIEYDTPARHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIVRVG
CCCCHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEHHHHHHHCCCCEEEECHHCCCEEEEC
DEVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSIT
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHCCCEEEECCCCCC
PHTTFKSEIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMT
CCCEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCEEEE
VTLIAPIAMDEGLRFAIREGGRTVGAGVVAEIVA
EEEEEEHHHCCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERG
CHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHCC
ITINTSHIEYDTPARHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIVRVG
CCCCHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEHHHHHHHCCCCEEEECHHCCCEEEEC
DEVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSIT
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHCCCEEEECCCCCC
PHTTFKSEIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMT
CCCEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCEEEE
VTLIAPIAMDEGLRFAIREGGRTVGAGVVAEIVA
EEEEEEHHHCCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA