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The map label for this gene is ycdT [H]

Identifier: 167623780

GI number: 167623780

Start: 2209861

End: 2210892

Strand: Direct

Name: ycdT [H]

Synonym: Shal_1850

Alternate gene names: 167623780

Gene position: 2209861-2210892 (Clockwise)

Preceding gene: 167623778

Following gene: 167623781

Centisome position: 42.28

GC content: 38.18

Gene sequence:

>1032_bases
ATGGATGGCAGCGGGTTAGATCTAACGAATAAAATAAGATCAAAAGTGCTCGCATCATTAAGTTTTGCGCTCCTTATTGC
GGCGATCTCATTTGCTATTTTTAATATTGTTTATCATCAAGCATACGCTTTTGCAGCTTTAGAACTTATTTTTGCTGCTT
TTTCATTTGATGTTTTCTGGAATAACAGTAGAAAGAATAATTACAGTGTTAATAAACTCTATTTTTACCTACTATTTTTG
GCTTTTCTGATCGTTTTTGGTACCTATAGCTTTCCATTAATCGATGCATTGTTCGTTTGGGCCTTCTCTTTCCCCATTAT
CTCCTATCTGCTTCTTGGAAAACGACTCGCTTTTCGAGTATCACTGTTTGTTTTCATCTCCATCGGTTTGATTCTTTTCT
ATAAATTAACCTTCCTTAGAGAGTTTGATATTGAACCTGTAATGATCAACTTTCTCTTCTGCTATACCTGTATCTGGACG
GTGTCACACTATTATGAACTCAGTAGAGCAAGTGCCCATGACTCATTAATTAAGCTGGCTCACAGTGATTCGCTGACCAA
AACTAATAACCGTTTAGCATTTTCTGAGGGATTTAATGACCATCAAGGAGAATATCTGTTGTTACTCGATCTTGATCACT
TTAAATCTATCAATGATACCTATGGCCACGACATTGGAGATCTGGTACTGGTATCTGTGGCCAACTGTTTTAAGACTTTG
ATAGATGAAAGCCAAGTTTTTCGCATTGGCGGTGAAGAGTTTTGTATTTGGTTAGGTACGAGTGATCTAGAAGGTGCACT
CGAACATTCTGAGAAGATAAGAAGGAGTGTCGCTCAACAGTCAATCGAATGCGATGAACAAAAGATTTCTATTACCGTTA
GCGGTGGCTTAGCTCAGCATCAACCTCAAATGCTGCAATCTGAATTGTTAAAAATGGCCGATAATCGTCTGTACTGTGCC
AAAAGTCACGGGAGGAACCTGATAGTCCATAAAGATGAAATGGAGGTGAGTAACCTTGTTTTGGTAAACTGA

Upstream 100 bases:

>100_bases
TGTAGTTGAGTTTATTTAATTATCGAATGGTTAACAACTTGTTGCGGGTGTAGAAGGTTGATATCATCACTCTAAAAGTA
TTACTTTTAGGGAAGGGAAC

Downstream 100 bases:

>100_bases
GCGATCTTTATTTTAACTGTTTTATTTCAACGGGTTGATGTGCTAGTTTGAATGTGTGGAGTAGATTAAATATGGTGATG
GTATCAGTCAGCTTAGCGCC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDGSGLDLTNKIRSKVLASLSFALLIAAISFAIFNIVYHQAYAFAALELIFAAFSFDVFWNNSRKNNYSVNKLYFYLLFL
AFLIVFGTYSFPLIDALFVWAFSFPIISYLLLGKRLAFRVSLFVFISIGLILFYKLTFLREFDIEPVMINFLFCYTCIWT
VSHYYELSRASAHDSLIKLAHSDSLTKTNNRLAFSEGFNDHQGEYLLLLDLDHFKSINDTYGHDIGDLVLVSVANCFKTL
IDESQVFRIGGEEFCIWLGTSDLEGALEHSEKIRRSVAQQSIECDEQKISITVSGGLAQHQPQMLQSELLKMADNRLYCA
KSHGRNLIVHKDEMEVSNLVLVN

Sequences:

>Translated_343_residues
MDGSGLDLTNKIRSKVLASLSFALLIAAISFAIFNIVYHQAYAFAALELIFAAFSFDVFWNNSRKNNYSVNKLYFYLLFL
AFLIVFGTYSFPLIDALFVWAFSFPIISYLLLGKRLAFRVSLFVFISIGLILFYKLTFLREFDIEPVMINFLFCYTCIWT
VSHYYELSRASAHDSLIKLAHSDSLTKTNNRLAFSEGFNDHQGEYLLLLDLDHFKSINDTYGHDIGDLVLVSVANCFKTL
IDESQVFRIGGEEFCIWLGTSDLEGALEHSEKIRRSVAQQSIECDEQKISITVSGGLAQHQPQMLQSELLKMADNRLYCA
KSHGRNLIVHKDEMEVSNLVLVN
>Mature_343_residues
MDGSGLDLTNKIRSKVLASLSFALLIAAISFAIFNIVYHQAYAFAALELIFAAFSFDVFWNNSRKNNYSVNKLYFYLLFL
AFLIVFGTYSFPLIDALFVWAFSFPIISYLLLGKRLAFRVSLFVFISIGLILFYKLTFLREFDIEPVMINFLFCYTCIWT
VSHYYELSRASAHDSLIKLAHSDSLTKTNNRLAFSEGFNDHQGEYLLLLDLDHFKSINDTYGHDIGDLVLVSVANCFKTL
IDESQVFRIGGEEFCIWLGTSDLEGALEHSEKIRRSVAQQSIECDEQKISITVSGGLAQHQPQMLQSELLKMADNRLYCA
KSHGRNLIVHKDEMEVSNLVLVN

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=34.5454545454545, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=40, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=37.3493975903614, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=37.1069182389937, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786584, Length=185, Percent_Identity=30.2702702702703, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=34.5029239766082, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082007, Length=174, Percent_Identity=35.0574712643678, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693134, Length=125, Percent_Identity=36, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=28.8235294117647, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39091; Mature: 39091

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGSGLDLTNKIRSKVLASLSFALLIAAISFAIFNIVYHQAYAFAALELIFAAFSFDVFW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
NNSRKNNYSVNKLYFYLLFLAFLIVFGTYSFPLIDALFVWAFSFPIISYLLLGKRLAFRV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLFVFISIGLILFYKLTFLREFDIEPVMINFLFCYTCIWTVSHYYELSRASAHDSLIKLA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSDSLTKTNNRLAFSEGFNDHQGEYLLLLDLDHFKSINDTYGHDIGDLVLVSVANCFKTL
CCCCCCCCCCEEEEECCCCCCCCCEEEEEECHHHCCCCHHCCCCHHHHHHHHHHHHHHHH
IDESQVFRIGGEEFCIWLGTSDLEGALEHSEKIRRSVAQQSIECDEQKISITVSGGLAQH
HCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
QPQMLQSELLKMADNRLYCAKSHGRNLIVHKDEMEVSNLVLVN
CHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCEEEEC
>Mature Secondary Structure
MDGSGLDLTNKIRSKVLASLSFALLIAAISFAIFNIVYHQAYAFAALELIFAAFSFDVFW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
NNSRKNNYSVNKLYFYLLFLAFLIVFGTYSFPLIDALFVWAFSFPIISYLLLGKRLAFRV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLFVFISIGLILFYKLTFLREFDIEPVMINFLFCYTCIWTVSHYYELSRASAHDSLIKLA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSDSLTKTNNRLAFSEGFNDHQGEYLLLLDLDHFKSINDTYGHDIGDLVLVSVANCFKTL
CCCCCCCCCCEEEEECCCCCCCCCEEEEEECHHHCCCCHHCCCCHHHHHHHHHHHHHHHH
IDESQVFRIGGEEFCIWLGTSDLEGALEHSEKIRRSVAQQSIECDEQKISITVSGGLAQH
HCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
QPQMLQSELLKMADNRLYCAKSHGRNLIVHKDEMEVSNLVLVN
CHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]