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The map label for this gene is tolB [H]

Identifier: 167623622

GI number: 167623622

Start: 2026321

End: 2027643

Strand: Direct

Name: tolB [H]

Synonym: Shal_1691

Alternate gene names: 167623622

Gene position: 2026321-2027643 (Clockwise)

Preceding gene: 167623621

Following gene: 167623623

Centisome position: 38.77

GC content: 45.2

Gene sequence:

>1323_bases
ATGAAAATTTTTGGGAAATGGTTGCTGGTAACCCTGCTTATTTGCAGTATGCCGGTAAAGGCTGCGTTAGATATTGTGAT
TACAGAAGGTATAGATGCTGCAAGACCTATTGCTGTGATCCCTTTTGTTTGGCAAGGTACTGGCCCAATGCCATCTCAGA
TCTCTGATGTTGTGATGTCAGATTTAGCTCGAAGCGGAACCTTTAGCCCAGCTGATGAGCTGAGTTTACCACAGCGTGGT
ATTAGTACCTTGGCGCAATTTAATGCTTCCGCTTGGATGACCCAGCCTGCTGAAGCCGTAGTCATGGGGTCGATTAAGCC
TTATGGCGGCGATAAGTATTTAGTGAGCTTTGAGTTAATCGATTTAGTTAAAGCGCAGCTTCAACCTGACTCGCAGTCGG
CACAAGATTTGGTAATTGATAGCCGGGAAACGATTATTACCGCGGCGCAGTTCCGTCAATATGGTCACCGCATTAGTGAC
GTTGTTTACGAAAAGCTTACAGGTATTCGTGGGGCGTTCTTAACTCGTATCGCCTATGTCGTTGTTAACCATGGTGAGAA
ATCTCCTTATAAGTTGATGATTAGTGACTATGACGGTTATAACGAACAGATGTTACTGCGCTCTCCTGAACCATTGATGT
CTCCTTCTTGGTCTCCGGATGGTCAGCGTCTTGCGTATGTCAGTTTTGAAAATCGTAAAGCGGAGATCTATGTACAAAAC
ATCTATACTCAGCAACGGACTAATGTTACCAGCTTCGATGGTATTAATGGCGCGCCCGTTTTTTCTCCTGATGGTAAAAA
ACTAGCGGTTACACTTTCTAAAGATGGTCAGCCTGAGGTTTACGTGGTTGATATCGCGACAAAGGCTATCAAACGTGTCA
CTAACCATTATGCGATTGACACCGAGCCATCTTGGTTCCCTGATGGTAAGTCATTACTTATCACCTCTGAGCGCGGCGGT
CGCCCTCAGCTATATCGTGTTTTCTTAGATTCAGGTAAGATTTCCCGTTTAACCTTCGAAGGTGAGTGGAATCTTGGCGG
TTCTATTGCACCTGATGGGCGCAGCATTATTTTTGTAAATCGCACCAATGGTAAGTTTAATATTGCCCGCATGGATCTAG
AGACTCGCTTTATGCAAGTGTTAACCAGCACGCGATTAGATGAATCTCCAAGCGTGGCGCCAAATGGCACCATGGTTATT
TACGGAACAACATACCAAGGCAAGCAGGTGTTAGCTGCGGTTTCTATGGATGGACGTTTTAAAGCAAGATTGCCCGTTGG
GCAGGGTGAAGTTAAATCACCATCTTGGTCACCATTTCTCTAA

Upstream 100 bases:

>100_bases
TAAAGCGGGTAGGTTACCTGTATCGAATGAGCCTGACGTTTATAACAAGCTCAAAGAAATCAATTTAACAGTTCAACCCG
AGTTCAATTAAAGGATCCAT

Downstream 100 bases:

>100_bases
CTAAAAAGATTAAGGATTTAAAAAATGGATCTGAATAAGCTATTTAAAGCCATGTTAGTCGTACTTCCTATTATGGCTAT
CAGTGCATGTAGCTCGACTT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKIFGKWLLVTLLICSMPVKAALDIVITEGIDAARPIAVIPFVWQGTGPMPSQISDVVMSDLARSGTFSPADELSLPQRG
ISTLAQFNASAWMTQPAEAVVMGSIKPYGGDKYLVSFELIDLVKAQLQPDSQSAQDLVIDSRETIITAAQFRQYGHRISD
VVYEKLTGIRGAFLTRIAYVVVNHGEKSPYKLMISDYDGYNEQMLLRSPEPLMSPSWSPDGQRLAYVSFENRKAEIYVQN
IYTQQRTNVTSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAIDTEPSWFPDGKSLLITSERGG
RPQLYRVFLDSGKISRLTFEGEWNLGGSIAPDGRSIIFVNRTNGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVI
YGTTYQGKQVLAAVSMDGRFKARLPVGQGEVKSPSWSPFL

Sequences:

>Translated_440_residues
MKIFGKWLLVTLLICSMPVKAALDIVITEGIDAARPIAVIPFVWQGTGPMPSQISDVVMSDLARSGTFSPADELSLPQRG
ISTLAQFNASAWMTQPAEAVVMGSIKPYGGDKYLVSFELIDLVKAQLQPDSQSAQDLVIDSRETIITAAQFRQYGHRISD
VVYEKLTGIRGAFLTRIAYVVVNHGEKSPYKLMISDYDGYNEQMLLRSPEPLMSPSWSPDGQRLAYVSFENRKAEIYVQN
IYTQQRTNVTSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAIDTEPSWFPDGKSLLITSERGG
RPQLYRVFLDSGKISRLTFEGEWNLGGSIAPDGRSIIFVNRTNGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVI
YGTTYQGKQVLAAVSMDGRFKARLPVGQGEVKSPSWSPFL
>Mature_440_residues
MKIFGKWLLVTLLICSMPVKAALDIVITEGIDAARPIAVIPFVWQGTGPMPSQISDVVMSDLARSGTFSPADELSLPQRG
ISTLAQFNASAWMTQPAEAVVMGSIKPYGGDKYLVSFELIDLVKAQLQPDSQSAQDLVIDSRETIITAAQFRQYGHRISD
VVYEKLTGIRGAFLTRIAYVVVNHGEKSPYKLMISDYDGYNEQMLLRSPEPLMSPSWSPDGQRLAYVSFENRKAEIYVQN
IYTQQRTNVTSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAIDTEPSWFPDGKSLLITSERGG
RPQLYRVFLDSGKISRLTFEGEWNLGGSIAPDGRSIIFVNRTNGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVI
YGTTYQGKQVLAAVSMDGRFKARLPVGQGEVKSPSWSPFL

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=429, Percent_Identity=44.7552447552448, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48603; Mature: 48603

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFGKWLLVTLLICSMPVKAALDIVITEGIDAARPIAVIPFVWQGTGPMPSQISDVVMS
CCCHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
DLARSGTFSPADELSLPQRGISTLAQFNASAWMTQPAEAVVMGSIKPYGGDKYLVSFELI
HHHHCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHHH
DLVKAQLQPDSQSAQDLVIDSRETIITAAQFRQYGHRISDVVYEKLTGIRGAFLTRIAYV
HHHHHHCCCCCCCCHHEEECCCHHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEE
VVNHGEKSPYKLMISDYDGYNEQMLLRSPEPLMSPSWSPDGQRLAYVSFENRKAEIYVQN
EEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEE
IYTQQRTNVTSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAID
EEHHHCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCEEEC
TEPSWFPDGKSLLITSERGGRPQLYRVFLDSGKISRLTFEGEWNLGGSIAPDGRSIIFVN
CCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCEECCCCCEEEEEE
RTNGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVIYGTTYQGKQVLAAVSMDGRF
CCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEE
KARLPVGQGEVKSPSWSPFL
EEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIFGKWLLVTLLICSMPVKAALDIVITEGIDAARPIAVIPFVWQGTGPMPSQISDVVMS
CCCHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
DLARSGTFSPADELSLPQRGISTLAQFNASAWMTQPAEAVVMGSIKPYGGDKYLVSFELI
HHHHCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHHH
DLVKAQLQPDSQSAQDLVIDSRETIITAAQFRQYGHRISDVVYEKLTGIRGAFLTRIAYV
HHHHHHCCCCCCCCHHEEECCCHHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEE
VVNHGEKSPYKLMISDYDGYNEQMLLRSPEPLMSPSWSPDGQRLAYVSFENRKAEIYVQN
EEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEE
IYTQQRTNVTSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAID
EEHHHCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCEEEC
TEPSWFPDGKSLLITSERGGRPQLYRVFLDSGKISRLTFEGEWNLGGSIAPDGRSIIFVN
CCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCEECCCCCEEEEEE
RTNGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVIYGTTYQGKQVLAAVSMDGRF
CCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEE
KARLPVGQGEVKSPSWSPFL
EEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA