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The map label for this gene is queA

Identifier: 167623484

GI number: 167623484

Start: 1873524

End: 1874561

Strand: Direct

Name: queA

Synonym: Shal_1553

Alternate gene names: 167623484

Gene position: 1873524-1874561 (Clockwise)

Preceding gene: 167623483

Following gene: 167623485

Centisome position: 35.84

GC content: 46.63

Gene sequence:

>1038_bases
ATGCGCGTTGCAGATTTTTCTTTTGAACTCCCTGACGAGTTAATCGCCCGTTACCCTACGGCCGAGCGAACAGCGTCCCG
GTTATTGTCACTCGATGGTAATAGCGGCGAGCTAACCGATCTACAGTTCACCGATATTTTAGAGCAAGTCAATCTTGGCG
ACTTGATGGTATTTAACAATACCCGAGTCATTCCTGCGCGTATGTTTGGTCAAAAACAATCTGGTGGAAAGCTCGAGATT
TTGGTTGAGCGTATGCTAGATGACAAGCGAGTCTTAGCCCATGTTCGTTGCTCTAAATCTCCAAAGGTCGATTCGATTAT
CTGCCTAGATGGCGGCTACGAGATGATAATGGTCGCTAGACATGACGCCCTGTTTGAACTCGAATTACAAAGCGATAAAA
CGATTTTAGAAGTACTTGAAGACGTCGGCCATATGCCATTGCCGCCTTATATCGATAGACCCGATGAAGACGCCGATAAA
GAACGTTACCAAACGGTTTATAATCAAAACCCTGGTGCGGTTGCGGCTCCTACGGCAGGTTTGCATTTTGATGATGCCAT
GCTTGCGGCATTAAAAGACAAAGGTGTTAACACTGCATTTGTGACCCTACACGTTGGTGCGGGTACCTTCCAACCTGTGC
GCGTAGATAATATACTCGATCATAAGATGCATTCGGAGTGGGCTGAAGTGCCACAGAATGTTGTGGATTTAATCGCTGAG
ACGAAAACCAGAGGCAATCGCGTTATTGCCGTTGGTACCACATCGGTACGTTCACTCGAAAGTGCAGCCAAAGCCTCGCC
TGAGCAGTTAGAAGCATTTAGTGGTGACACTGATATCTTTATCTACCCTGGATATCAGTTCCAAGTGGTCGATGCCATGG
TGACAAATTTCCACCTGCCAGAGTCGACTCTGATAATGTTGCTGAGTGCATTTGCCGGTTTCGATGAGGTAAAAAACGCC
TATCAGCACGCGATTGCTCAAAAATACCGCTTCTTTAGCTATGGCGACGCCATGTTTGTGACCAAAAAAGCGAACTAA

Upstream 100 bases:

>100_bases
TGTAAACGCGTATATTGACGGTTTTTTGAGGTCGGATTGTTGATCTTCTTTTGCTCTGGCCATAGACTAGCGGCCGATTT
AGACACAGTTGAGACTTTCC

Downstream 100 bases:

>100_bases
TTATCCAAATTGCTTTAAAATACCCAGTTCATATGAAGTGGGTATTTTTTTATCTGCACTTTATTAATGCATATTTTGGC
ATTTCGGGGGGATACTGTAT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRVADFSFELPDELIARYPTAERTASRLLSLDGNSGELTDLQFTDILEQVNLGDLMVFNNTRVIPARMFGQKQSGGKLEI
LVERMLDDKRVLAHVRCSKSPKVDSIICLDGGYEMIMVARHDALFELELQSDKTILEVLEDVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLAALKDKGVNTAFVTLHVGAGTFQPVRVDNILDHKMHSEWAEVPQNVVDLIAE
TKTRGNRVIAVGTTSVRSLESAAKASPEQLEAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLLSAFAGFDEVKNA
YQHAIAQKYRFFSYGDAMFVTKKAN

Sequences:

>Translated_345_residues
MRVADFSFELPDELIARYPTAERTASRLLSLDGNSGELTDLQFTDILEQVNLGDLMVFNNTRVIPARMFGQKQSGGKLEI
LVERMLDDKRVLAHVRCSKSPKVDSIICLDGGYEMIMVARHDALFELELQSDKTILEVLEDVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLAALKDKGVNTAFVTLHVGAGTFQPVRVDNILDHKMHSEWAEVPQNVVDLIAE
TKTRGNRVIAVGTTSVRSLESAAKASPEQLEAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLLSAFAGFDEVKNA
YQHAIAQKYRFFSYGDAMFVTKKAN
>Mature_345_residues
MRVADFSFELPDELIARYPTAERTASRLLSLDGNSGELTDLQFTDILEQVNLGDLMVFNNTRVIPARMFGQKQSGGKLEI
LVERMLDDKRVLAHVRCSKSPKVDSIICLDGGYEMIMVARHDALFELELQSDKTILEVLEDVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLAALKDKGVNTAFVTLHVGAGTFQPVRVDNILDHKMHSEWAEVPQNVVDLIAE
TKTRGNRVIAVGTTSVRSLESAAKASPEQLEAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLLSAFAGFDEVKNA
YQHAIAQKYRFFSYGDAMFVTKKAN

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=68.0232558139535, Blast_Score=489, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_SHEHH (B0TND3)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673778.1
- ProteinModelPortal:   B0TND3
- SMR:   B0TND3
- GeneID:   5902723
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1553
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   SHAL458817:SHAL_1553-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38398; Mature: 38398

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFSFELPDELIARYPTAERTASRLLSLDGNSGELTDLQFTDILEQVNLGDLMVFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHHEECCCCCCCEECCHHHHHHHHCCCCCEEEECC
TRVIPARMFGQKQSGGKLEILVERMLDDKRVLAHVRCSKSPKVDSIICLDGGYEMIMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEE
HDALFELELQSDKTILEVLEDVGHMPLPPYIDRPDEDADKERYQTVYNQNPGAVAAPTAG
CCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCC
LHFDDAMLAALKDKGVNTAFVTLHVGAGTFQPVRVDNILDHKMHSEWAEVPQNVVDLIAE
CCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TKTRGNRVIAVGTTSVRSLESAAKASPEQLEAFSGDTDIFIYPGYQFQVVDAMVTNFHLP
HCCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCEEEEHHHHHHHCCCC
ESTLIMLLSAFAGFDEVKNAYQHAIAQKYRFFSYGDAMFVTKKAN
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCC
>Mature Secondary Structure
MRVADFSFELPDELIARYPTAERTASRLLSLDGNSGELTDLQFTDILEQVNLGDLMVFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHHEECCCCCCCEECCHHHHHHHHCCCCCEEEECC
TRVIPARMFGQKQSGGKLEILVERMLDDKRVLAHVRCSKSPKVDSIICLDGGYEMIMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEE
HDALFELELQSDKTILEVLEDVGHMPLPPYIDRPDEDADKERYQTVYNQNPGAVAAPTAG
CCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCC
LHFDDAMLAALKDKGVNTAFVTLHVGAGTFQPVRVDNILDHKMHSEWAEVPQNVVDLIAE
CCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TKTRGNRVIAVGTTSVRSLESAAKASPEQLEAFSGDTDIFIYPGYQFQVVDAMVTNFHLP
HCCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCEEEEHHHHHHHCCCC
ESTLIMLLSAFAGFDEVKNAYQHAIAQKYRFFSYGDAMFVTKKAN
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA