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The map label for this gene is mraW [H]

Identifier: 167622387

GI number: 167622387

Start: 524745

End: 525692

Strand: Direct

Name: mraW [H]

Synonym: Shal_0447

Alternate gene names: 167622387

Gene position: 524745-525692 (Clockwise)

Preceding gene: 167622386

Following gene: 167622388

Centisome position: 10.04

GC content: 46.73

Gene sequence:

>948_bases
ATGACACAAGAATTTGAACACCTATCAGTACTGTTAACGGAAACGGTTGCAGGCTTAAACATAAAACCTGATGGCATCTA
TATTGATGGCACATTTGGTCGTGGTGGTCATTCTAGAAAAGTTCTTGAGGAACTTGGACCGAATGGAAGATTGATTGCTA
TTGACCGAGATCCACAGGCGATCGCAGCGGCTGAGCAGTTTAAAGATGATAGCCGCTTTCAAATTGTGCATGGTGGTTTC
GGTCAGTTGGCCGACTACGTCGAAGACTTAGGCCTGAAAGGCAAAATTGATGGTGTATTGTTGGACTTTGGCGTGTCATC
GCCGCAGTTAGATGATGCTGAGCGTGGTTTTAGTTTCTTAAGAGACGGCCCGTTAGATATGCGTATGGACAACTCTCAAG
GGCAAACTGCAGCGCAGTGGTTGGCGCGTGCCGAGATAGAAGACATGGCGTGGGTATTTAAAACCTATGGCGAAGAAAAA
AACTCTCGCCATATTGCGCGCTGTATTGCCGCCGACCGTGAGAAAGCCCCATTTTTACGTACCAAAGAACTGGCAGATTT
AATTGCTCGTATCACTAAGAACAAAGAGCGTAACAAGCATCCAGCTACTCGTGTATTCCAAGCTATCCGTATTTATATCA
ACAGTGAGCTTGAGCAGATTGACCAAGCACTAGAGGGCGCATTAACGGTATTGGCGCCTGAAGGGCGTTTATCTGTGATC
AGCTTCCATTCGCTTGAAGACCGTATGGTGAAACGTTTTATTCGTCGTAATAGCCAAGGTGAAAGTGTGCCTCATGGCCT
GCCTATCACTGAAGAAGAGATTAACAAATCACGTAAACTGAAGGGCGTAGGTAAGGCGATTAAGCCGTCAGCCGAAGAAA
TTGAGCGTAACGCTAGAGCGCGCAGCTCGGTGCTACGTATTGCCCAACGCTTACCTTACGAAGCATAA

Upstream 100 bases:

>100_bases
AGCAGAGTCGATTAGCGATTCTTAATGAAGATTTGGCTGCTAACGAACGTTTGGCAGATTTTTCACTTTAGCGAGTGATA
CTTAAAAGTAGAAGAAACCC

Downstream 100 bases:

>100_bases
GTGAGCTTGAGGCTAGCGTGAGTAAATCGCAATTGAATTTAACTCGAATTGTGCTGCTGGATCTTTGGCATCACAAATGG
GTAATGATTTTAGCATTGCT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTQEFEHLSVLLTETVAGLNIKPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQAIAAAEQFKDDSRFQIVHGGF
GQLADYVEDLGLKGKIDGVLLDFGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEK
NSRHIARCIAADREKAPFLRTKELADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALTVLAPEGRLSVI
SFHSLEDRMVKRFIRRNSQGESVPHGLPITEEEINKSRKLKGVGKAIKPSAEEIERNARARSSVLRIAQRLPYEA

Sequences:

>Translated_315_residues
MTQEFEHLSVLLTETVAGLNIKPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQAIAAAEQFKDDSRFQIVHGGF
GQLADYVEDLGLKGKIDGVLLDFGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEK
NSRHIARCIAADREKAPFLRTKELADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALTVLAPEGRLSVI
SFHSLEDRMVKRFIRRNSQGESVPHGLPITEEEINKSRKLKGVGKAIKPSAEEIERNARARSSVLRIAQRLPYEA
>Mature_314_residues
TQEFEHLSVLLTETVAGLNIKPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQAIAAAEQFKDDSRFQIVHGGFG
QLADYVEDLGLKGKIDGVLLDFGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEKN
SRHIARCIAADREKAPFLRTKELADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALTVLAPEGRLSVIS
FHSLEDRMVKRFIRRNSQGESVPHGLPITEEEINKSRKLKGVGKAIKPSAEEIERNARARSSVLRIAQRLPYEA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=37.0919881305638, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI165377202, Length=194, Percent_Identity=39.1752577319588, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=63.1410256410256, Blast_Score=402, Evalue=1e-113,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=40.8408408408408, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=40.8408408408408, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35260; Mature: 35128

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEFEHLSVLLTETVAGLNIKPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQA
CCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHH
IAAAEQFKDDSRFQIVHGGFGQLADYVEDLGLKGKIDGVLLDFGVSSPQLDDAERGFSFL
HHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEKNSRHIARCIAADREKAPFLR
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHH
TKELADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALTVLAPEGRLSVI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEE
SFHSLEDRMVKRFIRRNSQGESVPHGLPITEEEINKSRKLKGVGKAIKPSAEEIERNARA
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RSSVLRIAQRLPYEA
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQEFEHLSVLLTETVAGLNIKPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQA
CCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHH
IAAAEQFKDDSRFQIVHGGFGQLADYVEDLGLKGKIDGVLLDFGVSSPQLDDAERGFSFL
HHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEKNSRHIARCIAADREKAPFLR
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHH
TKELADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALTVLAPEGRLSVI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEE
SFHSLEDRMVKRFIRRNSQGESVPHGLPITEEEINKSRKLKGVGKAIKPSAEEIERNARA
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RSSVLRIAQRLPYEA
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA