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The map label for this gene is glpK

Identifier: 167622384

GI number: 167622384

Start: 521778

End: 523259

Strand: Direct

Name: glpK

Synonym: Shal_0444

Alternate gene names: 167622384

Gene position: 521778-523259 (Clockwise)

Preceding gene: 167622383

Following gene: 167622386

Centisome position: 9.98

GC content: 48.52

Gene sequence:

>1482_bases
GTGTCAAAGAAATACATCATTGCACTGGACCAAGGTACAACCAGTTCTCGAGCCATAGTGTTTGATCACAGTACTAATAT
GGTCGCTAGTTGTCAGCGTGAGTTTAGCCAGATGTACCCTAAACCGGGCTGGGTTGAACATGATGCAATGGAGATTTGGG
CTTCGCAAAGCTCGACTCTCATTGAGGCATTAGCCCGAGCCGACATTCATAGTGAAGATGTTGCCGCCATCGGTATTACT
AACCAGCGTGAAACCACGGTGATATGGGATAAGGCCACAGGCAAGCCCGTTTACAATGCAATCGTGTGGCAGTGCCGTCG
TAGTAAAGCTATCTGTGATGAGCTAAAAACACAGGGGCTTGAAGATTATGTAAAGCAAGCAACAGGTTTACTACTCGACC
CCTATTTCTCTGGCACCAAAATTAAGTGGATTTTAGATAATGTCGATGGGGTAAGAGAGCGTGCTGAAAAAGGTGAGTTA
CTGTTTGGCACCATTGATACCTGGTTGGTATGGAAGCTAACCGAGGGGCAAGCCCATGTTACCGATCCGACTAATGCATC
ACGTACCATGTTATTTAATATCCACACCCAAGAGTGGGATGAGACTCTGCTTAAGGCGCTCAATATTCCGCGCTCAATAT
TACCTGAGGTAAAACCTTCATCGGCGGTTTATGGTCATACCCGTATCGTGGGTGAAGGTAGCCATATTGCTATTGCGGGT
ATGGCGGGCGATCAGCAGGCGGCGCTATTTGGTCAGCTTTGCATTGAAGAGGGCATGGCCAAAAATACGTACGGTACCGG
GTGTTTCCTGTTGATGAACACTGGTACTGAAGCCGTTCAGTCACAACATGGGTTATTAACCACCATCGCAATTGGGGCTA
ATGGCGAAGTGAATTACGCGCTAGAAGGCTCGGTATTTATGGGCGGTGCTACGGTGCAGTGGCTTAGAGATGAGCTTGGG
CTTATTCGTGATGCGCAAGATACCGAGTACTTTGCCTCTAAGGTTGAAGATACTCACGGGGTATATTTAATTCCTGCATT
TGTTGGCTTGGGCGCACCATATTGGGATCCGGACGCAAGAGGAGCCTTAGTGGGGTTAACTCGAGGCGCTAATCGTAACC
ATATCATCAGGGCCGCACTTGAGGCGATCGCCTATCAAAGTCGTGATTTGCTCGATGCCATGGCTAAAGATAGTCGTGTT
GAACTTAAGCAGATTAAGGTCGATGGCGGTGCGGTGGCGAATGATTTCTTGATGCAGTTTCAGTCAGATATTACCAATGT
CGAAGTTCAGCGACCAGATTTGACTGAAACCACGGCCATGGGCGCAGCATTTTTAGCTGGGTTGGCGGTCGGGTTTTGGG
ATTCAACGGATGAACTAAAGCTTAAGGCCGGAGTGGAGCGCAGCTTTACGCCGCAGATAACGGCACAAAAGCGCGATAGT
TTATACCAAGGTTGGCAAAATGCGGTGGCTCGGACTCGATAA

Upstream 100 bases:

>100_bases
ATGCTTGTGATGAAACCATATGGGTAGATGGCAGTGATCCTGAAATTGTTCAGCCAGAGATCGGCCTATTAAAAGAAACG
CGTCAATAAGGAAAGCATAA

Downstream 100 bases:

>100_bases
GTCATAACGGCAACTACTTAAACTGAAACTATACATTGATGTCGATCTTAGTGTGGGTCGACTCTTTTAGTTTCGTTGAG
GACTCTCTCTGCTTTGCAGA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSKKYIIALDQGTTSSRAIVFDHSTNMVASCQREFSQMYPKPGWVEHDAMEIWASQSSTLIEALARADIHSEDVAAIGIT
NQRETTVIWDKATGKPVYNAIVWQCRRSKAICDELKTQGLEDYVKQATGLLLDPYFSGTKIKWILDNVDGVRERAEKGEL
LFGTIDTWLVWKLTEGQAHVTDPTNASRTMLFNIHTQEWDETLLKALNIPRSILPEVKPSSAVYGHTRIVGEGSHIAIAG
MAGDQQAALFGQLCIEEGMAKNTYGTGCFLLMNTGTEAVQSQHGLLTTIAIGANGEVNYALEGSVFMGGATVQWLRDELG
LIRDAQDTEYFASKVEDTHGVYLIPAFVGLGAPYWDPDARGALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMAKDSRV
ELKQIKVDGGAVANDFLMQFQSDITNVEVQRPDLTETTAMGAAFLAGLAVGFWDSTDELKLKAGVERSFTPQITAQKRDS
LYQGWQNAVARTR

Sequences:

>Translated_493_residues
MSKKYIIALDQGTTSSRAIVFDHSTNMVASCQREFSQMYPKPGWVEHDAMEIWASQSSTLIEALARADIHSEDVAAIGIT
NQRETTVIWDKATGKPVYNAIVWQCRRSKAICDELKTQGLEDYVKQATGLLLDPYFSGTKIKWILDNVDGVRERAEKGEL
LFGTIDTWLVWKLTEGQAHVTDPTNASRTMLFNIHTQEWDETLLKALNIPRSILPEVKPSSAVYGHTRIVGEGSHIAIAG
MAGDQQAALFGQLCIEEGMAKNTYGTGCFLLMNTGTEAVQSQHGLLTTIAIGANGEVNYALEGSVFMGGATVQWLRDELG
LIRDAQDTEYFASKVEDTHGVYLIPAFVGLGAPYWDPDARGALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMAKDSRV
ELKQIKVDGGAVANDFLMQFQSDITNVEVQRPDLTETTAMGAAFLAGLAVGFWDSTDELKLKAGVERSFTPQITAQKRDS
LYQGWQNAVARTR
>Mature_492_residues
SKKYIIALDQGTTSSRAIVFDHSTNMVASCQREFSQMYPKPGWVEHDAMEIWASQSSTLIEALARADIHSEDVAAIGITN
QRETTVIWDKATGKPVYNAIVWQCRRSKAICDELKTQGLEDYVKQATGLLLDPYFSGTKIKWILDNVDGVRERAEKGELL
FGTIDTWLVWKLTEGQAHVTDPTNASRTMLFNIHTQEWDETLLKALNIPRSILPEVKPSSAVYGHTRIVGEGSHIAIAGM
AGDQQAALFGQLCIEEGMAKNTYGTGCFLLMNTGTEAVQSQHGLLTTIAIGANGEVNYALEGSVFMGGATVQWLRDELGL
IRDAQDTEYFASKVEDTHGVYLIPAFVGLGAPYWDPDARGALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMAKDSRVE
LKQIKVDGGAVANDFLMQFQSDITNVEVQRPDLTETTAMGAAFLAGLAVGFWDSTDELKLKAGVERSFTPQITAQKRDSL
YQGWQNAVARTR

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI42794763, Length=514, Percent_Identity=45.9143968871595, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI189409120, Length=509, Percent_Identity=46.1689587426326, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI4504007, Length=509, Percent_Identity=46.1689587426326, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI41393575, Length=498, Percent_Identity=46.5863453815261, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI88196792, Length=504, Percent_Identity=33.7301587301587, Blast_Score=305, Evalue=9e-83,
Organism=Escherichia coli, GI1790361, Length=490, Percent_Identity=68.5714285714286, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=522, Percent_Identity=26.2452107279693, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789138, Length=499, Percent_Identity=23.4468937875752, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790005, Length=513, Percent_Identity=23.0019493177388, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1789987, Length=488, Percent_Identity=24.3852459016393, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=498, Percent_Identity=42.1686746987952, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71986140, Length=519, Percent_Identity=25.2408477842004, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71986147, Length=297, Percent_Identity=29.96632996633, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=479, Percent_Identity=39.4572025052192, Blast_Score=305, Evalue=7e-84,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=43.9453125, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24654573, Length=437, Percent_Identity=46.2242562929062, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=39.5582329317269, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=39.5582329317269, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=39.5582329317269, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=39.5582329317269, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=39.5582329317269, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI19922058, Length=521, Percent_Identity=33.0134357005758, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24652871, Length=488, Percent_Identity=34.0163934426229, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24656367, Length=518, Percent_Identity=29.5366795366795, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI21357867, Length=533, Percent_Identity=28.7054409005629, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24656372, Length=389, Percent_Identity=30.8483290488432, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45552909, Length=330, Percent_Identity=27.8787878787879, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_SHEHH (B0TQM6)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672678.1
- ProteinModelPortal:   B0TQM6
- SMR:   B0TQM6
- GeneID:   5905631
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0444
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   CLSK907562
- BioCyc:   SHAL458817:SHAL_0444-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54203; Mature: 54072

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKYIIALDQGTTSSRAIVFDHSTNMVASCQREFSQMYPKPGWVEHDAMEIWASQSSTL
CCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHH
IEALARADIHSEDVAAIGITNQRETTVIWDKATGKPVYNAIVWQCRRSKAICDELKTQGL
HHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
EDYVKQATGLLLDPYFSGTKIKWILDNVDGVRERAEKGELLFGTIDTWLVWKLTEGQAHV
HHHHHHHCCEEEECCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECEEEEEEEECCCEEE
TDPTNASRTMLFNIHTQEWDETLLKALNIPRSILPEVKPSSAVYGHTRIVGEGSHIAIAG
CCCCCCCEEEEEEEECHHHHHHHHHHHCCCHHHCCCCCCCCEEECCEEEEECCCEEEEEE
MAGDQQAALFGQLCIEEGMAKNTYGTGCFLLMNTGTEAVQSQHGLLTTIAIGANGEVNYA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEECCCCEEEEE
LEGSVFMGGATVQWLRDELGLIRDAQDTEYFASKVEDTHGVYLIPAFVGLGAPYWDPDAR
EECCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCC
GALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMAKDSRVELKQIKVDGGAVANDFLMQF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHH
QSDITNVEVQRPDLTETTAMGAAFLAGLAVGFWDSTDELKLKAGVERSFTPQITAQKRDS
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHH
LYQGWQNAVARTR
HHHHHHHHHHCCC
>Mature Secondary Structure 
SKKYIIALDQGTTSSRAIVFDHSTNMVASCQREFSQMYPKPGWVEHDAMEIWASQSSTL
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHH
IEALARADIHSEDVAAIGITNQRETTVIWDKATGKPVYNAIVWQCRRSKAICDELKTQGL
HHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
EDYVKQATGLLLDPYFSGTKIKWILDNVDGVRERAEKGELLFGTIDTWLVWKLTEGQAHV
HHHHHHHCCEEEECCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECEEEEEEEECCCEEE
TDPTNASRTMLFNIHTQEWDETLLKALNIPRSILPEVKPSSAVYGHTRIVGEGSHIAIAG
CCCCCCCEEEEEEEECHHHHHHHHHHHCCCHHHCCCCCCCCEEECCEEEEECCCEEEEEE
MAGDQQAALFGQLCIEEGMAKNTYGTGCFLLMNTGTEAVQSQHGLLTTIAIGANGEVNYA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEECCCCEEEEE
LEGSVFMGGATVQWLRDELGLIRDAQDTEYFASKVEDTHGVYLIPAFVGLGAPYWDPDAR
EECCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCC
GALVGLTRGANRNHIIRAALEAIAYQSRDLLDAMAKDSRVELKQIKVDGGAVANDFLMQF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHH
QSDITNVEVQRPDLTETTAMGAAFLAGLAVGFWDSTDELKLKAGVERSFTPQITAQKRDS
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHH
LYQGWQNAVARTR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA