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The map label for this gene is silB [H]

Identifier: 167622220

GI number: 167622220

Start: 336478

End: 338160

Strand: Reverse

Name: silB [H]

Synonym: Shal_0279

Alternate gene names: 167622220

Gene position: 338160-336478 (Counterclockwise)

Preceding gene: 167622221

Following gene: 167622219

Centisome position: 6.47

GC content: 47.89

Gene sequence:

>1683_bases
ATGAGTTTTTTTATATCAAAAAAAATGCGACAAACTACAGTTTGTGCACTGAGTTTAGTGATGGCATCTACCCCGCTAAT
GGGGTTCAGCCAAACAACTGAAACAACTAACAGTAAACAGGCTCAAGCTCACCAGCATGAATCAAGTCAACATGAGTCTG
AGTACTACTGCCCCATGCACCCTGAGGTGACCAGCCATGAGCCCGGTCGCTGTCCAGAATGTAAGATGTTCCTCGTGAAA
GACGAAGCTAATAGTAGTACTTCTGAAGCAGCCGCTATAGTCGAAAACTACTACTGCCCCATGCACCCTGAAGTGACCAG
TCATGAGCCCGGTCGCTGTCCAGAATGTAAGATGTTCCTCGTAAAAGAGGAAGAAGAGGAAACTCTCCCTGATGAGCATG
TTGGTCATAACCACGCTGATAAACAACACAAGAACTCTCAACAGCAGACCAATAAAACTGCAGCTGATAAGATCTTAAGT
AAGCCCAAGCCTACTCTTATACCTGCTGCACAGAGTAACGGTGACGGCAACATCAAGTATGTTTGCCCGATGCACCCTCA
TGTTGTCTCTGATGTACCTGGCACCTGTCCAATATGCGGCATGGATCTGGAAAAAGTCACTATCGGTGGTGTCGGCGAAG
AGGTTGTCGTCGGTGTTTCTGGCGGAATGCAGCAAGCGCTCGGCATGCGCAGTGAAGAGGTTACACAAGGCACCCTATGG
AAGCTGGTCAAAACTATCGGCACGGTAGAGTACAACGAGAATGCCATAGGTCACAGCCACACACGCGTTAACGGTTGGAT
AGAAACCTTAAAGGTGCATAACGTCGGCCAGCGAGTAAAGAAGGGGCAATTACTTTATGAGCTTTATTCACCAGAGCTCA
TCAATGCTCAAGACGATTACATGCAAGCGATGGACTACCTTAAGCAAGATAAAGATCGCGGCCAAGCGTTGCTTCGCAAG
GCGCGTTTACGCCTTGAGTTATTAGGGGTTAGCTCTGACACGATTAAACAGTTGGAACGCACTGGAAAAACCATTTATCG
CGTGCCATTTTATGCAGAGCAAGACGGTTTTATCAGTAAATTAACTGTGCGTCATGGCATGTATGTACAACCTGGCGATA
CCCTATTTGAGATTGTTGACTTAAGCAGTGTCTGGGTCATCGCCGATGTGTTTGAGAATGAGCAGAGCTGGCTAGAGCAA
GGTCGCCCTGTGGAGGTGACTTCTGCCGCTCAAGGGCTTTTCGATATCGAGTCAAGCATTGACTATATCTATCCAGAACT
GGATCCCGTATCGCGCGCAATGCGAGTCAGGATCAAGCTAGACAATCCCGATAAGCTTCTCAAGCCCGGCACCTTAGTCG
ATGTAAAACTGTTCGGAGGTCCGAAGCAGGAAGTGCTATCGATTCCAACTGAAGCGCTGATCCTCACTGGCCGCGAAAAT
CGTGTCGTAGTACAACGTGACGATAACCGCTTTGCTTCTGTTCCGGTCAAAGTGGGTATGATTGCCCAAGGTAAGGCTGA
AATTATTGAAGGCCTTCAGGTCGGCGATAAAGTGATTGTCTCTGGTCAGTTCTTACTGGATTCAGAAGCCAGTATTCAAG
GTAGCTTACAGCGCTTAAGTGGCAGTAACAGTGGCAGTAACAGCAATAGCAGCGCAGCTAGCGATCCACACGCAAACCAT
TAA

Upstream 100 bases:

>100_bases
CCCTAAATGTGGTATGGACTTAGAACCTATCGCGACAGGCGATAAAGCGACTAAATCTGATCACATGCATCACTAATATT
TAGGGTGGAGATAAGGCATT

Downstream 100 bases:

>100_bases
TCGGAGGCAGTCATGTTAGAAAGAATTATCAGCGCCTCGATTAAACAGCGGGCGATGGTGTTAGTACTTACTGCGGTGAT
TGCCTTAATCGGTTATCAAG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSFFISKKMRQTTVCALSLVMASTPLMGFSQTTETTNSKQAQAHQHESSQHESEYYCPMHPEVTSHEPGRCPECKMFLVK
DEANSSTSEAAAIVENYYCPMHPEVTSHEPGRCPECKMFLVKEEEEETLPDEHVGHNHADKQHKNSQQQTNKTAADKILS
KPKPTLIPAAQSNGDGNIKYVCPMHPHVVSDVPGTCPICGMDLEKVTIGGVGEEVVVGVSGGMQQALGMRSEEVTQGTLW
KLVKTIGTVEYNENAIGHSHTRVNGWIETLKVHNVGQRVKKGQLLYELYSPELINAQDDYMQAMDYLKQDKDRGQALLRK
ARLRLELLGVSSDTIKQLERTGKTIYRVPFYAEQDGFISKLTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWLEQ
GRPVEVTSAAQGLFDIESSIDYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLFGGPKQEVLSIPTEALILTGREN
RVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLQVGDKVIVSGQFLLDSEASIQGSLQRLSGSNSGSNSNSSAASDPHANH

Sequences:

>Translated_560_residues
MSFFISKKMRQTTVCALSLVMASTPLMGFSQTTETTNSKQAQAHQHESSQHESEYYCPMHPEVTSHEPGRCPECKMFLVK
DEANSSTSEAAAIVENYYCPMHPEVTSHEPGRCPECKMFLVKEEEEETLPDEHVGHNHADKQHKNSQQQTNKTAADKILS
KPKPTLIPAAQSNGDGNIKYVCPMHPHVVSDVPGTCPICGMDLEKVTIGGVGEEVVVGVSGGMQQALGMRSEEVTQGTLW
KLVKTIGTVEYNENAIGHSHTRVNGWIETLKVHNVGQRVKKGQLLYELYSPELINAQDDYMQAMDYLKQDKDRGQALLRK
ARLRLELLGVSSDTIKQLERTGKTIYRVPFYAEQDGFISKLTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWLEQ
GRPVEVTSAAQGLFDIESSIDYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLFGGPKQEVLSIPTEALILTGREN
RVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLQVGDKVIVSGQFLLDSEASIQGSLQRLSGSNSGSNSNSSAASDPHANH
>Mature_559_residues
SFFISKKMRQTTVCALSLVMASTPLMGFSQTTETTNSKQAQAHQHESSQHESEYYCPMHPEVTSHEPGRCPECKMFLVKD
EANSSTSEAAAIVENYYCPMHPEVTSHEPGRCPECKMFLVKEEEEETLPDEHVGHNHADKQHKNSQQQTNKTAADKILSK
PKPTLIPAAQSNGDGNIKYVCPMHPHVVSDVPGTCPICGMDLEKVTIGGVGEEVVVGVSGGMQQALGMRSEEVTQGTLWK
LVKTIGTVEYNENAIGHSHTRVNGWIETLKVHNVGQRVKKGQLLYELYSPELINAQDDYMQAMDYLKQDKDRGQALLRKA
RLRLELLGVSSDTIKQLERTGKTIYRVPFYAEQDGFISKLTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWLEQG
RPVEVTSAAQGLFDIESSIDYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLFGGPKQEVLSIPTEALILTGRENR
VVVQRDDNRFASVPVKVGMIAQGKAEIIEGLQVGDKVIVSGQFLLDSEASIQGSLQRLSGSNSGSNSNSSAASDPHANH

Specific function: Component of the sil cation efflux system that confers resistance to silver. May be part of a three-component cation/proton antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=369, Percent_Identity=31.1653116531165, Blast_Score=150, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 61905; Mature: 61774

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFISKKMRQTTVCALSLVMASTPLMGFSQTTETTNSKQAQAHQHESSQHESEYYCPMH
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
PEVTSHEPGRCPECKMFLVKDEANSSTSEAAAIVENYYCPMHPEVTSHEPGRCPECKMFL
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
VKEEEEETLPDEHVGHNHADKQHKNSQQQTNKTAADKILSKPKPTLIPAAQSNGDGNIKY
EECCHHHCCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEE
VCPMHPHVVSDVPGTCPICGMDLEKVTIGGVGEEVVVGVSGGMQQALGMRSEEVTQGTLW
ECCCCCHHHCCCCCCCCEECCCCCEEEECCCCCEEEEECCCCHHHHHCCCHHHHHHHHHH
KLVKTIGTVEYNENAIGHSHTRVNGWIETLKVHNVGQRVKKGQLLYELYSPELINAQDDY
HHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHH
MQAMDYLKQDKDRGQALLRKARLRLELLGVSSDTIKQLERTGKTIYRVPFYAEQDGFISK
HHHHHHHHCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCEEEEECEEECCCCCCEE
LTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWLEQGRPVEVTSAAQGLFDIESSI
EEHHCCCEECCCCHHHHHHCCCCCEEEHHHHCCHHHHHHCCCCEEEEHHHCCHHHHHCCC
DYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLFGGPKQEVLSIPTEALILTGREN
CEECCCCCCCCCEEEEEEEECCCHHHCCCCCEEEEEECCCCHHHHHHCCCEEEEEECCCC
RVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLQVGDKVIVSGQFLLDSEASIQGSLQRLS
EEEEEECCCCEEECCEEEEEECCCHHHHHHHHHCCCEEEEECEEEECCCCHHHHHHHHHC
GSNSGSNSNSSAASDPHANH
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFFISKKMRQTTVCALSLVMASTPLMGFSQTTETTNSKQAQAHQHESSQHESEYYCPMH
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
PEVTSHEPGRCPECKMFLVKDEANSSTSEAAAIVENYYCPMHPEVTSHEPGRCPECKMFL
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
VKEEEEETLPDEHVGHNHADKQHKNSQQQTNKTAADKILSKPKPTLIPAAQSNGDGNIKY
EECCHHHCCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEE
VCPMHPHVVSDVPGTCPICGMDLEKVTIGGVGEEVVVGVSGGMQQALGMRSEEVTQGTLW
ECCCCCHHHCCCCCCCCEECCCCCEEEECCCCCEEEEECCCCHHHHHCCCHHHHHHHHHH
KLVKTIGTVEYNENAIGHSHTRVNGWIETLKVHNVGQRVKKGQLLYELYSPELINAQDDY
HHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHH
MQAMDYLKQDKDRGQALLRKARLRLELLGVSSDTIKQLERTGKTIYRVPFYAEQDGFISK
HHHHHHHHCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCEEEEECEEECCCCCCEE
LTVRHGMYVQPGDTLFEIVDLSSVWVIADVFENEQSWLEQGRPVEVTSAAQGLFDIESSI
EEHHCCCEECCCCHHHHHHCCCCCEEEHHHHCCHHHHHHCCCCEEEEHHHCCHHHHHCCC
DYIYPELDPVSRAMRVRIKLDNPDKLLKPGTLVDVKLFGGPKQEVLSIPTEALILTGREN
CEECCCCCCCCCEEEEEEEECCCHHHCCCCCEEEEEECCCCHHHHHHCCCEEEEEECCCC
RVVVQRDDNRFASVPVKVGMIAQGKAEIIEGLQVGDKVIVSGQFLLDSEASIQGSLQRLS
EEEEEECCCCEEECCEEEEEECCCHHHHHHHHHCCCEEEEECEEEECCCCHHHHHHHHHC
GSNSGSNSNSSAASDPHANH
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9930866 [H]