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The map label for this gene is narX [H]

Identifier: 167622116

GI number: 167622116

Start: 203201

End: 204961

Strand: Reverse

Name: narX [H]

Synonym: Shal_0175

Alternate gene names: 167622116

Gene position: 204961-203201 (Counterclockwise)

Preceding gene: 167622123

Following gene: 167622115

Centisome position: 3.92

GC content: 43.78

Gene sequence:

>1761_bases
ATGGCTGAACCCGATAACGTAAAACACCTACCGTTGGTTCAATCGGAAGATAACGACAATGTATCTTCATCCTTAGTCGC
TACCGCCGGTTTATACATGGCCACAATCATCTCTCTCGCCCTGATGAGCATGATTTTTTCAATTCTGGTAACCGAAAAAC
TGGCTGGTGGAGCCGAATTAATAAACAATACGGGTTCGCTGAGAATGCAAGTGATCCGCATATCTCGAGCTCAATTCTTA
GAAACAGGAAGCGCAAACCATTTAATAGGGGAGGAAAAAAAAGCTTTTGAAACCAAACTATTCAAACTAAAGCAAACAAC
CTTAGGAACCAGCTTCAACACCCCTGAGATCGAGAAGCAATATCAACAAATTGTCTCACAGTGGCAGCAATTAAATTCTG
CGCCTAAAGCTGCACTGCCAGAGGATTACGATCAGTTCACCAGTAAGTTAGATAATCTTGTTCATTTAATTCAACTTGAA
TCAGAGAAAAAACTCACCCTATTACGGCTGATTCAAAGTATTGCCGTATTGCTTATTCTTGTTATCAGTACAATCGCTCT
AATCAAGATAAATCAACTACTTGCAGCACCACTTAAGAATCTAGTGCATGTAGCCGAAGAGGCGGGAAAGGGAAACTTCA
GTGCACCGATAGAGTTTGAAGCGGATAATGAGCTTGGCTTACTGGCCAAGACCATCAAGCGGATGTCGAGTCAGCTGGCG
TATACCTACCAAGACTATGAACAGATAGTGGCAGAAAAAACTCGCGAACTCACTCGCAGCAATGAATCTTTAGAGGTGCT
TTACAAGGGTGCAAGACTACTATCGAATCAAGAGTATGACCGCGTTAAGTCAGTTATTATTCAAGATATGCAAGCCCTTA
TCGGTATCGGCACGATAAAGATAAGATTGATCCATAAACATATCGATAATCTAGTCGTGTTTCAGGCAGGAGAATCCGGC
CACCCCTCCAATTACAAACATAGTTATCAGTTCTCTCTAGAAAAGCATAACAAGGTCTTTGGCGATATCATTTGGGAGGT
GCCAGAAGGTCAATCTGTTGAGGACTGGCAGAAACAGTTGATTCAAGCCATGGCTGATATTGTTGCAACCGCGATAGAGT
TAGGGCATCAAAAAAATGCAGAAAACAGGCTACTCATTTCGGAAGAGCGTGCCATTATTGCCAGAGAGCTGCATGATTCA
TTGGCGCAATCACTGTCATTCTTGAAAGTTCAAATGAGCCTGCTAACGCGTAAAATGGAGAAGAAACTCCCCGAAGAGCA
AATTGAAGAGACCATAGTCGAGATCAAGAGCGGTCTCAATAAAGCTTATCTGCAACTGCGAGAACTACTAACCACCTTCA
GACTCAAACTTGACGACCCCTCTATAGAAAGTGCTCTGCAGGGCACAATTGCCGAATTCACTCAGAAGTGCCAACACCCG
ATACGCTTCAGCTACGACCTGCCACAACACTGTTTATCTGCCAACCAAGAAATTCATCTACTGCAGATCATCAGAGAGGG
GCTGTCAAATATTCACCGTCACGCCAAGGCCTCCGAGGCAGGGGTTAGATTATCGCCAAAAGGGGAACTAATTAAGCTCG
ATATATGGGATAATGGCGCAGGCTTACCTGGTACGCTTCAAACTCAAGGCCACTTTGGCCTAGGGATCATGAGAGAGAGA
GTGAGTTCTCTGAAGGGGCAAATGTACCTAAAAGATCACTTACCTTCGGGCACTATGATTTCAATTACGTTCGAGAATTA
G

Upstream 100 bases:

>100_bases
GTTGAAAACTGATTTTTTCCTATAACCGTTCTACCAAAACAACTATTTGGTAGTAGTTAGCGCCCTAATACGAAAATCAC
TTAACAAAAGGCAGCGAAAA

Downstream 100 bases:

>100_bases
ATTACCGGATAAAGGATTAGAAAAATAAGATGCCTATAGCCAAAATACTACTCGTTGATGACCACCCCGCCCTACGCCAA
GGACTCGCGCAGCTCATGTC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MAEPDNVKHLPLVQSEDNDNVSSSLVATAGLYMATIISLALMSMIFSILVTEKLAGGAELINNTGSLRMQVIRISRAQFL
ETGSANHLIGEEKKAFETKLFKLKQTTLGTSFNTPEIEKQYQQIVSQWQQLNSAPKAALPEDYDQFTSKLDNLVHLIQLE
SEKKLTLLRLIQSIAVLLILVISTIALIKINQLLAAPLKNLVHVAEEAGKGNFSAPIEFEADNELGLLAKTIKRMSSQLA
YTYQDYEQIVAEKTRELTRSNESLEVLYKGARLLSNQEYDRVKSVIIQDMQALIGIGTIKIRLIHKHIDNLVVFQAGESG
HPSNYKHSYQFSLEKHNKVFGDIIWEVPEGQSVEDWQKQLIQAMADIVATAIELGHQKNAENRLLISEERAIIARELHDS
LAQSLSFLKVQMSLLTRKMEKKLPEEQIEETIVEIKSGLNKAYLQLRELLTTFRLKLDDPSIESALQGTIAEFTQKCQHP
IRFSYDLPQHCLSANQEIHLLQIIREGLSNIHRHAKASEAGVRLSPKGELIKLDIWDNGAGLPGTLQTQGHFGLGIMRER
VSSLKGQMYLKDHLPSGTMISITFEN

Sequences:

>Translated_586_residues
MAEPDNVKHLPLVQSEDNDNVSSSLVATAGLYMATIISLALMSMIFSILVTEKLAGGAELINNTGSLRMQVIRISRAQFL
ETGSANHLIGEEKKAFETKLFKLKQTTLGTSFNTPEIEKQYQQIVSQWQQLNSAPKAALPEDYDQFTSKLDNLVHLIQLE
SEKKLTLLRLIQSIAVLLILVISTIALIKINQLLAAPLKNLVHVAEEAGKGNFSAPIEFEADNELGLLAKTIKRMSSQLA
YTYQDYEQIVAEKTRELTRSNESLEVLYKGARLLSNQEYDRVKSVIIQDMQALIGIGTIKIRLIHKHIDNLVVFQAGESG
HPSNYKHSYQFSLEKHNKVFGDIIWEVPEGQSVEDWQKQLIQAMADIVATAIELGHQKNAENRLLISEERAIIARELHDS
LAQSLSFLKVQMSLLTRKMEKKLPEEQIEETIVEIKSGLNKAYLQLRELLTTFRLKLDDPSIESALQGTIAEFTQKCQHP
IRFSYDLPQHCLSANQEIHLLQIIREGLSNIHRHAKASEAGVRLSPKGELIKLDIWDNGAGLPGTLQTQGHFGLGIMRER
VSSLKGQMYLKDHLPSGTMISITFEN
>Mature_585_residues
AEPDNVKHLPLVQSEDNDNVSSSLVATAGLYMATIISLALMSMIFSILVTEKLAGGAELINNTGSLRMQVIRISRAQFLE
TGSANHLIGEEKKAFETKLFKLKQTTLGTSFNTPEIEKQYQQIVSQWQQLNSAPKAALPEDYDQFTSKLDNLVHLIQLES
EKKLTLLRLIQSIAVLLILVISTIALIKINQLLAAPLKNLVHVAEEAGKGNFSAPIEFEADNELGLLAKTIKRMSSQLAY
TYQDYEQIVAEKTRELTRSNESLEVLYKGARLLSNQEYDRVKSVIIQDMQALIGIGTIKIRLIHKHIDNLVVFQAGESGH
PSNYKHSYQFSLEKHNKVFGDIIWEVPEGQSVEDWQKQLIQAMADIVATAIELGHQKNAENRLLISEERAIIARELHDSL
AQSLSFLKVQMSLLTRKMEKKLPEEQIEETIVEIKSGLNKAYLQLRELLTTFRLKLDDPSIESALQGTIAEFTQKCQHPI
RFSYDLPQHCLSANQEIHLLQIIREGLSNIHRHAKASEAGVRLSPKGELIKLDIWDNGAGLPGTLQTQGHFGLGIMRERV
SSLKGQMYLKDHLPSGTMISITFEN

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=585, Percent_Identity=28.034188034188, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788812, Length=584, Percent_Identity=31.3356164383562, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI87082311, Length=215, Percent_Identity=30.6976744186047, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 65770; Mature: 65639

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPDNVKHLPLVQSEDNDNVSSSLVATAGLYMATIISLALMSMIFSILVTEKLAGGAEL
CCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
INNTGSLRMQVIRISRAQFLETGSANHLIGEEKKAFETKLFKLKQTTLGTSFNTPEIEKQ
HCCCCHHEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
YQQIVSQWQQLNSAPKAALPEDYDQFTSKLDNLVHLIQLESEKKLTLLRLIQSIAVLLIL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VISTIALIKINQLLAAPLKNLVHVAEEAGKGNFSAPIEFEADNELGLLAKTIKRMSSQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
YTYQDYEQIVAEKTRELTRSNESLEVLYKGARLLSNQEYDRVKSVIIQDMQALIGIGTIK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHE
IRLIHKHIDNLVVFQAGESGHPSNYKHSYQFSLEKHNKVFGDIIWEVPEGQSVEDWQKQL
EHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHEECCCCCCHHHHHHHH
IQAMADIVATAIELGHQKNAENRLLISEERAIIARELHDSLAQSLSFLKVQMSLLTRKME
HHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLPEEQIEETIVEIKSGLNKAYLQLRELLTTFRLKLDDPSIESALQGTIAEFTQKCQHP
HHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
IRFSYDLPQHCLSANQEIHLLQIIREGLSNIHRHAKASEAGVRLSPKGELIKLDIWDNGA
CEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCC
GLPGTLQTQGHFGLGIMRERVSSLKGQMYLKDHLPSGTMISITFEN
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECC
>Mature Secondary Structure 
AEPDNVKHLPLVQSEDNDNVSSSLVATAGLYMATIISLALMSMIFSILVTEKLAGGAEL
CCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
INNTGSLRMQVIRISRAQFLETGSANHLIGEEKKAFETKLFKLKQTTLGTSFNTPEIEKQ
HCCCCHHEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
YQQIVSQWQQLNSAPKAALPEDYDQFTSKLDNLVHLIQLESEKKLTLLRLIQSIAVLLIL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VISTIALIKINQLLAAPLKNLVHVAEEAGKGNFSAPIEFEADNELGLLAKTIKRMSSQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
YTYQDYEQIVAEKTRELTRSNESLEVLYKGARLLSNQEYDRVKSVIIQDMQALIGIGTIK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHE
IRLIHKHIDNLVVFQAGESGHPSNYKHSYQFSLEKHNKVFGDIIWEVPEGQSVEDWQKQL
EHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHEECCCCCCHHHHHHHH
IQAMADIVATAIELGHQKNAENRLLISEERAIIARELHDSLAQSLSFLKVQMSLLTRKME
HHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLPEEQIEETIVEIKSGLNKAYLQLRELLTTFRLKLDDPSIESALQGTIAEFTQKCQHP
HHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
IRFSYDLPQHCLSANQEIHLLQIIREGLSNIHRHAKASEAGVRLSPKGELIKLDIWDNGA
CEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCC
GLPGTLQTQGHFGLGIMRERVSSLKGQMYLKDHLPSGTMISITFEN
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]