Click here to switch to the map view.

The map label for this gene is moaE [H]

Identifier: 167622067

GI number: 167622067

Start: 142165

End: 142617

Strand: Direct

Name: moaE [H]

Synonym: Shal_0126

Alternate gene names: 167622067

Gene position: 142165-142617 (Clockwise)

Preceding gene: 167622066

Following gene: 167622068

Centisome position: 2.72

GC content: 47.9

Gene sequence:

>453_bases
ATGATCCGTGTTCAGACCCAAGACTTTAATGTCCCAGAGGAATATCACTTAATCAGCCAAGATAACAGCGATGGTGCGGT
AGTGACGTTTGTCGGTAAGGTACGTGACTTTAACGATGGCAGTGCAGTGACCGATCTAACGCTTGAGCATTACCCTGGCA
TGACAGAAGCGGTGTTGAATCAGATTGAAGTCGAGGCGCGTGAGCGTTGGCCGCTAAACAAGGTCACCATTATTCATCGT
GTTGGTGCCATGGCTCTGGGTGAGCAGATCGTATTTATTGGTGTCACGAGTGCTCATCGCAAGGCAGCCTTTGCTGCTTG
TGAGTTTTTGATCGATTTTCTTAAGACAAAAGCACCGTTTTGGAAATTAGAAGCGGGTGATAAAGGCGCAAACTGGGTTG
AAGCCCGTGACGCCGATGAACAAGCCGCAAAAATGTGGGATAAAAAAGCGTAG

Upstream 100 bases:

>100_bases
AAGCTATTGGTTGCAGTAAATCAAACCATCAGCCAGTGGGATACGCCGATTGTTGATGGTGACGAAGTGGCATTCTTCCC
ACCAGTAACTGGAGGTTAAG

Downstream 100 bases:

>100_bases
CAAACGCTTTAATTCAAGGAGTTCAGCTCAATGAAGTTTAGGGTTATGGTGCTTGCTCTATTGCTGCCCTTTTTAGCAAT
AAGTAACGGCGCGTCAGCGG

Product: molybdopterin biosynthesis MoaE protein

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2 [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLNQIEVEARERWPLNKVTIIHR
VGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGDKGANWVEARDADEQAAKMWDKKA

Sequences:

>Translated_150_residues
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLNQIEVEARERWPLNKVTIIHR
VGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGDKGANWVEARDADEQAAKMWDKKA
>Mature_150_residues
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLNQIEVEARERWPLNKVTIIHR
VGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGDKGANWVEARDADEQAAKMWDKKA

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD [H]

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family [H]

Homologues:

Organism=Homo sapiens, GI4758732, Length=108, Percent_Identity=36.1111111111111, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787003, Length=145, Percent_Identity=59.3103448275862, Blast_Score=183, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17509279, Length=108, Percent_Identity=38.8888888888889, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356259, Length=126, Percent_Identity=35.7142857142857, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003448 [H]

Pfam domain/function: PF02391 MoaE [H]

EC number: NA

Molecular weight: Translated: 16808; Mature: 16808

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLN
CEEEECCCCCCCHHEEEEECCCCCCEEEEEEECCEECCCCCEEEEEEHHHCCCHHHHHHH
QIEVEARERWPLNKVTIIHRVGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPF
HHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCE
WKLEAGDKGANWVEARDADEQAAKMWDKKA
EEEECCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLN
CEEEECCCCCCCHHEEEEECCCCCCEEEEEEECCEECCCCCEEEEEEHHHCCCHHHHHHH
QIEVEARERWPLNKVTIIHRVGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPF
HHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCE
WKLEAGDKGANWVEARDADEQAAKMWDKKA
EEEECCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]