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The map label for this gene is rhtB [H]

Identifier: 167622027

GI number: 167622027

Start: 96832

End: 97458

Strand: Reverse

Name: rhtB [H]

Synonym: Shal_0086

Alternate gene names: 167622027

Gene position: 97458-96832 (Counterclockwise)

Preceding gene: 167622028

Following gene: 167622025

Centisome position: 1.86

GC content: 47.53

Gene sequence:

>627_bases
ATGAGCTTTGATACTTGGTTACTCTACCTGTTTGCCATTTTGTTAATTGCAGTGTCTCCAGGCACCATGGCTGTATTGTC
TATGGGTCACGGCATTCATTATGGTAAGAGCCGCAGCATAGCCACTGCATTTGGCAGTGTGACTTCAGCATTGATATTAA
TGATGGCATCGGCTGCGGGCTTAGGTGCACTGCTAAGTGCAACAGAATATGGCTTTACGGTATTAAAATGGTGTGGCGCA
GCTTACCTGCTGTTTCTGGGCATAAAATTACTGCTCACTAAAGGTGATGGCAAAGGGCTGCAACTAAAAACCACCGAAGG
CAAGGGCACACCTAAGCAACTATTCAAGCAAGCCTTTCTCGTTGGGATCAGCAACCCTAAAGATCTGCTATTTTTTGCTG
CGCTGTTTCCTCAGTTTATCGACATCAGTGCGCCTCAAGGCCCACAGTTAGCCATCTTGGCTGCAACTTGGGCGGTAGTG
GATTTCAGCTTTGTGATGATCTACGCCAGCATGGCGAATGTATTGGCACCAAAATTAAAGGCGAGCAACAAGCTGCATTG
GTTCGACAGAACCAGTGGAGGGGTATTTGTGGCATTAGCCGCTATCTTGGTAAGCCGCGAGAACTAG

Upstream 100 bases:

>100_bases
TTAAAGGCTAGGCTCATTGCTGACTTTATTTCAGTCTAGATTGCCTAAATAATGTCTGGCGGCTATAGTGCTGCCAGTCA
TTTTAAATTGTATAAAATCT

Downstream 100 bases:

>100_bases
CTTTCAATAGGTAAAAAATTTAAATAACGACTTTCTGAGCAGGCAAAGCCTAATTGATGATAACTCGCTACTGAAATAGG
TGATTCAAGCCTGAAAATAA

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSFDTWLLYLFAILLIAVSPGTMAVLSMGHGIHYGKSRSIATAFGSVTSALILMMASAAGLGALLSATEYGFTVLKWCGA
AYLLFLGIKLLLTKGDGKGLQLKTTEGKGTPKQLFKQAFLVGISNPKDLLFFAALFPQFIDISAPQGPQLAILAATWAVV
DFSFVMIYASMANVLAPKLKASNKLHWFDRTSGGVFVALAAILVSREN

Sequences:

>Translated_208_residues
MSFDTWLLYLFAILLIAVSPGTMAVLSMGHGIHYGKSRSIATAFGSVTSALILMMASAAGLGALLSATEYGFTVLKWCGA
AYLLFLGIKLLLTKGDGKGLQLKTTEGKGTPKQLFKQAFLVGISNPKDLLFFAALFPQFIDISAPQGPQLAILAATWAVV
DFSFVMIYASMANVLAPKLKASNKLHWFDRTSGGVFVALAAILVSREN
>Mature_207_residues
SFDTWLLYLFAILLIAVSPGTMAVLSMGHGIHYGKSRSIATAFGSVTSALILMMASAAGLGALLSATEYGFTVLKWCGAA
YLLFLGIKLLLTKGDGKGLQLKTTEGKGTPKQLFKQAFLVGISNPKDLLFFAALFPQFIDISAPQGPQLAILAATWAVVD
FSFVMIYASMANVLAPKLKASNKLHWFDRTSGGVFVALAAILVSREN

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=208, Percent_Identity=33.1730769230769, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788099, Length=160, Percent_Identity=32.5, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22187; Mature: 22055

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDTWLLYLFAILLIAVSPGTMAVLSMGHGIHYGKSRSIATAFGSVTSALILMMASAAG
CCHHHHHHHHHHHHHHHCCCCHHEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LGALLSATEYGFTVLKWCGAAYLLFLGIKLLLTKGDGKGLQLKTTEGKGTPKQLFKQAFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCHHHHHHHHHH
VGISNPKDLLFFAALFPQFIDISAPQGPQLAILAATWAVVDFSFVMIYASMANVLAPKLK
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNKLHWFDRTSGGVFVALAAILVSREN
CCCCEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFDTWLLYLFAILLIAVSPGTMAVLSMGHGIHYGKSRSIATAFGSVTSALILMMASAAG
CHHHHHHHHHHHHHHHCCCCHHEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LGALLSATEYGFTVLKWCGAAYLLFLGIKLLLTKGDGKGLQLKTTEGKGTPKQLFKQAFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCHHHHHHHHHH
VGISNPKDLLFFAALFPQFIDISAPQGPQLAILAATWAVVDFSFVMIYASMANVLAPKLK
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNKLHWFDRTSGGVFVALAAILVSREN
CCCCEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]