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The map label for this gene is hisS

Identifier: 16752377

GI number: 16752377

Start: 97395

End: 98687

Strand: Direct

Name: hisS

Synonym: CP0084

Alternate gene names: 16752377

Gene position: 97395-98687 (Clockwise)

Preceding gene: 16752376

Following gene: 16752378

Centisome position: 7.92

GC content: 41.14

Gene sequence:

>1293_bases
GTGACTGTAACTCTCCCCAAAGGGGTCTTTGATATATTTCCTTATCTTGCAGATGCTAAACAATTGTGGCGTCACACTTC
ACTTTGGCATAGTGTTGAGAAGGCAATTCATACCGTTTGCATGCTTTATGGATTTTGTGAAATTCGTACTCCTATTTTTG
AAAAATCAGAAGTGTTTTTACATGTAGGGGAAGAAAGTGATGTTGTTAAAAAAGAAGTCTATTCGTTTTTAGATAGAAAA
GGTCGTTCCATGACTTTGCGTCCCGAAGGGACTGCCGCTGTTGTCCGTTCTTTTCTTGAACACGGGGCCTCTCATCGAAG
TGATAATAAGTTCTATTATATTCTTCCCATGTTTCGCTACGAACGTCAGCAAGCGGGTAGATATCGTCAACACCATCAGT
TCGGTGTTGAGGCTATTGGTGTGCGTCACCCTCTAAGAGATGCAGAAGTTCTCGCTTTGCTTTGGGATTTCTACTCTCGT
GTCGGTTTGCAACATATGCAAATTCAACTCAATTTCTTAGGAGGAAGTGAGACAAGATTTCGATACGATAAGGTTCTACG
CGCTTATTTGAAAGAGTCCATGGGAGAATTATCGGCATTAAGCCAGCAGAGATTTTCAACGAATGTTTTGCGTATTTTGG
ATTCAAAGGAGCCTGAAGATCAAGAAATTATCCGTCAAGCGCCCCCGATTCTAGACTATGTTTCTGATGAAGATCTTAAG
TATTTTAATGAAATTTTAGACGCTTTGAGAGTTTTAGAAATCCCATATGCTATCAATCCTCGTTTAGTGCGTGGTTTGGA
TTATTATTCAGACTTAGTCTTTGAAGCGACTACCACATTCCAAGAGGTCTCTTATGCCTTAGGGGGAGGCGGGCGCTATG
ACGGCTTGATTTCAGCTTTTGGAGGAGCTTCTCTTCCTGCCTGTGGTTTCGGTGTTGGCCTTGAAAGAGCGATTCAAACG
TTATTAGCTCAAAAGCGTATTGAGCCACAGTTCCCTCATAAACTGCGTTTGATTCCAATGGAACCAGATGCGGATCAGTT
TTGTTTGGAATGGTCGCAACATTTGCGACGGTTGGGAATCCCTACGGAAGTCGATTGGTCTCATAAAAAAGTAAAAGGAG
CTCTAAAAGCAGCGAGTACAGAACAGGTTTCTTTCGTTTGCTTAATCGGTGAGCGAGAGTTAATTTCTCAACAGTTAGTT
ATTAAAAATATGTCTCTGCGTAAAGAATTCTTTGGAACAAAAGAAGAAGTAGAGCAAAGGTTGTTATATGAAATACAGAA
CACACCGTTGTAA

Upstream 100 bases:

>100_bases
GTAGATTCGAGTGTGAATATGACTTAGGCTTAGGCAGGAGTCACTAAGCTCCAGATATTCCATTATCTAAAAAAGTAATC
ATTTTGAGCGAAGACATCAC

Downstream 100 bases:

>100_bases
TGAACTCACAAGTAATCATATAGGTGAGAATGTTCAATTGGCAGGATGGGTGCACCGTTATCGTAATCATGGGGGTGTTG
TCTTCATAGATTTGCGAGAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFLHVGEESDVVKKEVYSFLDRK
GRSMTLRPEGTAAVVRSFLEHGASHRSDNKFYYILPMFRYERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSR
VGLQHMQIQLNFLGGSETRFRYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLK
YFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAFGGASLPACGFGVGLERAIQT
LLAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGIPTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLV
IKNMSLRKEFFGTKEEVEQRLLYEIQNTPL

Sequences:

>Translated_430_residues
MTVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFLHVGEESDVVKKEVYSFLDRK
GRSMTLRPEGTAAVVRSFLEHGASHRSDNKFYYILPMFRYERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSR
VGLQHMQIQLNFLGGSETRFRYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLK
YFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAFGGASLPACGFGVGLERAIQT
LLAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGIPTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLV
IKNMSLRKEFFGTKEEVEQRLLYEIQNTPL
>Mature_429_residues
TVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFLHVGEESDVVKKEVYSFLDRKG
RSMTLRPEGTAAVVRSFLEHGASHRSDNKFYYILPMFRYERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSRV
GLQHMQIQLNFLGGSETRFRYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLKY
FNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAFGGASLPACGFGVGLERAIQTL
LAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGIPTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLVI
KNMSLRKEFFGTKEEVEQRLLYEIQNTPL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=387, Percent_Identity=37.2093023255814, Blast_Score=258, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_CHLPN (Q9Z7P1)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D86573
- PIR:   H72052
- RefSeq:   NP_224859.1
- RefSeq:   NP_300719.1
- RefSeq:   NP_444636.1
- RefSeq:   NP_876961.1
- ProteinModelPortal:   Q9Z7P1
- SMR:   Q9Z7P1
- GeneID:   1467368
- GeneID:   895245
- GeneID:   919377
- GeneID:   963773
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0084
- KEGG:   cpj:CPj0663
- KEGG:   cpn:CPn0663
- KEGG:   cpt:CpB0689
- TIGR:   CP_0084
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q9Z7P1
- ProtClustDB:   PRK00037
- BioCyc:   CPNE115711:CP_0084-MONOMER
- BioCyc:   CPNE115713:CPN0663-MONOMER
- BioCyc:   CPNE138677:CPJ0663-MONOMER
- BioCyc:   CPNE182082:CPB0689-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 49552; Mature: 49420

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFL
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
HVGEESDVVKKEVYSFLDRKGRSMTLRPEGTAAVVRSFLEHGASHRSDNKFYYILPMFRY
EECCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEECHHHH
ERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSRVGLQHMQIQLNFLGGSETRF
HHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHH
RYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHCCCHHHH
YFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAF
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
GGASLPACGFGVGLERAIQTLLAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
PTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLVIKNMSLRKEFFGTKEEVEQR
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLYEIQNTPL
HHHHHHCCCC
>Mature Secondary Structure 
TVTLPKGVFDIFPYLADAKQLWRHTSLWHSVEKAIHTVCMLYGFCEIRTPIFEKSEVFL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
HVGEESDVVKKEVYSFLDRKGRSMTLRPEGTAAVVRSFLEHGASHRSDNKFYYILPMFRY
EECCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEECHHHH
ERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSRVGLQHMQIQLNFLGGSETRF
HHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHH
RYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPEDQEIIRQAPPILDYVSDEDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHCCCHHHH
YFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFEATTTFQEVSYALGGGGRYDGLISAF
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
GGASLPACGFGVGLERAIQTLLAQKRIEPQFPHKLRLIPMEPDADQFCLEWSQHLRRLGI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
PTEVDWSHKKVKGALKAASTEQVSFVCLIGERELISQQLVIKNMSLRKEFFGTKEEVEQR
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLYEIQNTPL
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362