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The map label for this gene is pdhD [H]

Identifier: 167040661

GI number: 167040661

Start: 2047713

End: 2049065

Strand: Reverse

Name: pdhD [H]

Synonym: Teth514_2038

Alternate gene names: 167040661

Gene position: 2049065-2047713 (Counterclockwise)

Preceding gene: 167040663

Following gene: 167040660

Centisome position: 83.39

GC content: 36.66

Gene sequence:

>1353_bases
ATGGATTACGATGTTATAGTTTTAGGCGGAGGGCCTGGTGGCTATACTGCTGCTATTAGACTCAGCGAATTAGGGAAAAA
AGTCGCTGTTGTAGAAGAAGATTCATTAGGGGGAACTTGTTTAAATCGAGGTTGTATACCGACGAAAGTCTATTCTCATG
CGGCTGAGCTTATAAATGCCATAAAAGATGCTAAAGACTTTGGAATAATGGCCCAGTATGCAGTGGATATTGCAAAACTT
CGTCAGAAAAAAGAAAGAGTAGTAAAAAGACTTGTCGGTGGAGTAGGCTATTTAATGAACTTACACCATATAGATGTTAT
AAAAGGGAGAGGTAGGTTTGTTGATGAAAATACTATAGAAGTTGATAAAAGATACACAGCAGAAAATTTTATAATTGCAA
CAGGTTCAAAGGTGTTTTTACCTCCTATTGAAGGGATTAACTTAGAAGGTGTTATTACAAGTGATAAAGCGTTAGAACTT
GAAAGAATTCCTGAAAAAATTGTCATCATAGGGGCGGGAATTATAGGATTGGAATTTGCAAATATTTATTCTGCTTTAGG
AAGTAAAGTTGTCATCATTGAAATGCTTCCTCAACTTTTGCCTATGTTAGATAGAGACATAGCAGATACGATGGAAAAAA
TTTTGAGGCATAAAAAAATTGAACTACATTTAAACAGTAAAGTAGAAAAGATAGAAGAAGGTTTAAAGGTAGTTTATACC
ACAGAAGGGAATACTCAGGTAGTGGAATGTGATACTGTTTTAGTCGCTGTAGGAAGAGTTGCCAATGTAAATGGAATTGA
AGCTTTAAATCTTGATATGGATAAAAAGGGCATAAAAGTCGACTCCCACATGAGGACAAGCATAAAAAATATATATGCAA
TTGGAGATGTTACAGGAGGTATACAACTGGCTCACGTGGCTTCTTATCAAGGTATTGTAGCTGCTCACAATATAGCAGGG
GAAGAAAAGGAAGCGGATTTAAGTATAGTTCCAAATTGTCTCTATACAAATCCTGAAATAGCGTGGGCAGGTTTGAATGA
GGTTCAAGCAAGAGAAAAATTTGGAGATGTAAAAATAGGCACTTTCCCTTATACTGCTTTAGGAAGAGCAATGACTATGG
GGCAAAACGATGGATTTGTTAAAATAATTGCGGAGGCAAAATACAATAGAGTAGTGGGGATGGAAATAATAGGGGCTGGT
GCTACAGAGATAATTCATGAGGGTGTACTTGCCATAAAAGAAGAGTTCACATTAGAAGAATTAGCAGATGCAATTCATGC
TCATCCTACGCTTTCAGAAAGCGTAAAAGAGGCAGCAGAAGACGCATTAGGAATGCCTATAAATAAAGGATAA

Upstream 100 bases:

>100_bases
ACATGTTATTGACAATGATGAAAGAAGGATTTTGAGGTTAAGTGTAGAAATAAATAAAAAGATAAATCAAGAATCAATAC
ACTCAAAAAGGAGGAGAAAA

Downstream 100 bases:

>100_bases
GTTAAAGGAGAGATTCACTTGAGAAAAGGAGAAGTGTTAAAACTGGGAATTGTACCTTATATGGAAGGAAAAGAGATACA
GCTTAAAGCCTTTGAAAGAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDYDVIVLGGGPGGYTAAIRLSELGKKVAVVEEDSLGGTCLNRGCIPTKVYSHAAELINAIKDAKDFGIMAQYAVDIAKL
RQKKERVVKRLVGGVGYLMNLHHIDVIKGRGRFVDENTIEVDKRYTAENFIIATGSKVFLPPIEGINLEGVITSDKALEL
ERIPEKIVIIGAGIIGLEFANIYSALGSKVVIIEMLPQLLPMLDRDIADTMEKILRHKKIELHLNSKVEKIEEGLKVVYT
TEGNTQVVECDTVLVAVGRVANVNGIEALNLDMDKKGIKVDSHMRTSIKNIYAIGDVTGGIQLAHVASYQGIVAAHNIAG
EEKEADLSIVPNCLYTNPEIAWAGLNEVQAREKFGDVKIGTFPYTALGRAMTMGQNDGFVKIIAEAKYNRVVGMEIIGAG
ATEIIHEGVLAIKEEFTLEELADAIHAHPTLSESVKEAAEDALGMPINKG

Sequences:

>Translated_450_residues
MDYDVIVLGGGPGGYTAAIRLSELGKKVAVVEEDSLGGTCLNRGCIPTKVYSHAAELINAIKDAKDFGIMAQYAVDIAKL
RQKKERVVKRLVGGVGYLMNLHHIDVIKGRGRFVDENTIEVDKRYTAENFIIATGSKVFLPPIEGINLEGVITSDKALEL
ERIPEKIVIIGAGIIGLEFANIYSALGSKVVIIEMLPQLLPMLDRDIADTMEKILRHKKIELHLNSKVEKIEEGLKVVYT
TEGNTQVVECDTVLVAVGRVANVNGIEALNLDMDKKGIKVDSHMRTSIKNIYAIGDVTGGIQLAHVASYQGIVAAHNIAG
EEKEADLSIVPNCLYTNPEIAWAGLNEVQAREKFGDVKIGTFPYTALGRAMTMGQNDGFVKIIAEAKYNRVVGMEIIGAG
ATEIIHEGVLAIKEEFTLEELADAIHAHPTLSESVKEAAEDALGMPINKG
>Mature_450_residues
MDYDVIVLGGGPGGYTAAIRLSELGKKVAVVEEDSLGGTCLNRGCIPTKVYSHAAELINAIKDAKDFGIMAQYAVDIAKL
RQKKERVVKRLVGGVGYLMNLHHIDVIKGRGRFVDENTIEVDKRYTAENFIIATGSKVFLPPIEGINLEGVITSDKALEL
ERIPEKIVIIGAGIIGLEFANIYSALGSKVVIIEMLPQLLPMLDRDIADTMEKILRHKKIELHLNSKVEKIEEGLKVVYT
TEGNTQVVECDTVLVAVGRVANVNGIEALNLDMDKKGIKVDSHMRTSIKNIYAIGDVTGGIQLAHVASYQGIVAAHNIAG
EEKEADLSIVPNCLYTNPEIAWAGLNEVQAREKFGDVKIGTFPYTALGRAMTMGQNDGFVKIIAEAKYNRVVGMEIIGAG
ATEIIHEGVLAIKEEFTLEELADAIHAHPTLSESVKEAAEDALGMPINKG

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=37.037037037037, Blast_Score=296, Evalue=4e-80,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=33.6263736263736, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI148277065, Length=450, Percent_Identity=30.4444444444444, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI33519430, Length=450, Percent_Identity=30.4444444444444, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI33519428, Length=450, Percent_Identity=30.4444444444444, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI33519426, Length=450, Percent_Identity=30.4444444444444, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI148277071, Length=450, Percent_Identity=30.4444444444444, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=30.0420168067227, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI291045266, Length=474, Percent_Identity=28.6919831223629, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI291045268, Length=466, Percent_Identity=27.6824034334764, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1786307, Length=450, Percent_Identity=39.7777777777778, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI87081717, Length=449, Percent_Identity=31.8485523385301, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI87082354, Length=457, Percent_Identity=30.4157549234136, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789915, Length=427, Percent_Identity=31.615925058548, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1789065, Length=256, Percent_Identity=24.609375, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788892, Length=209, Percent_Identity=29.6650717703349, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=36.6228070175439, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI71983419, Length=430, Percent_Identity=28.3720930232558, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71983429, Length=430, Percent_Identity=28.3720930232558, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=29.0870488322718, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=481, Percent_Identity=29.3139293139293, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=463, Percent_Identity=37.7969762419007, Blast_Score=269, Evalue=9e-73,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=30.1724137931034, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=28.1573498964803, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=37.7398720682303, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24640549, Length=471, Percent_Identity=30.3609341825902, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24640553, Length=471, Percent_Identity=30.3609341825902, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640551, Length=471, Percent_Identity=30.3609341825902, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=28.3298097251586, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48751; Mature: 48751

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYDVIVLGGGPGGYTAAIRLSELGKKVAVVEEDSLGGTCLNRGCIPTKVYSHAAELINA
CCEEEEEEECCCCCEEEEEEHHHCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHH
IKDAKDFGIMAQYAVDIAKLRQKKERVVKRLVGGVGYLMNLHHIDVIKGRGRFVDENTIE
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEECCCCCEECCCCEE
VDKRYTAENFIIATGSKVFLPPIEGINLEGVITSDKALELERIPEKIVIIGAGIIGLEFA
ECCEECCCEEEEEECCEEEECCCCCCCEEEEEECCCCHHHHHCCCEEEEEECCHHHHHHH
NIYSALGSKVVIIEMLPQLLPMLDRDIADTMEKILRHKKIELHLNSKVEKIEEGLKVVYT
HHHHHHCCCEEHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEEECHHHHHHHCCCEEEEE
TEGNTQVVECDTVLVAVGRVANVNGIEALNLDMDKKGIKVDSHMRTSIKNIYAIGDVTGG
ECCCCEEEECCEEEEECCCCCCCCCCEEEECCCCCCCCEECHHHHHHHHHEEEEECCCCC
IQLAHVASYQGIVAAHNIAGEEKEADLSIVPNCLYTNPEIAWAGLNEVQAREKFGDVKIG
EEEEEECCCCCEEEEECCCCCCCCCCCEECCCEEECCCCEEECCCHHHHHHHHHCCEEEC
TFPYTALGRAMTMGQNDGFVKIIAEAKYNRVVGMEIIGAGATEIIHEGVLAIKEEFTLEE
CCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHH
LADAIHAHPTLSESVKEAAEDALGMPINKG
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDYDVIVLGGGPGGYTAAIRLSELGKKVAVVEEDSLGGTCLNRGCIPTKVYSHAAELINA
CCEEEEEEECCCCCEEEEEEHHHCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHH
IKDAKDFGIMAQYAVDIAKLRQKKERVVKRLVGGVGYLMNLHHIDVIKGRGRFVDENTIE
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEECCCCCEECCCCEE
VDKRYTAENFIIATGSKVFLPPIEGINLEGVITSDKALELERIPEKIVIIGAGIIGLEFA
ECCEECCCEEEEEECCEEEECCCCCCCEEEEEECCCCHHHHHCCCEEEEEECCHHHHHHH
NIYSALGSKVVIIEMLPQLLPMLDRDIADTMEKILRHKKIELHLNSKVEKIEEGLKVVYT
HHHHHHCCCEEHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEEECHHHHHHHCCCEEEEE
TEGNTQVVECDTVLVAVGRVANVNGIEALNLDMDKKGIKVDSHMRTSIKNIYAIGDVTGG
ECCCCEEEECCEEEEECCCCCCCCCCEEEECCCCCCCCEECHHHHHHHHHEEEEECCCCC
IQLAHVASYQGIVAAHNIAGEEKEADLSIVPNCLYTNPEIAWAGLNEVQAREKFGDVKIG
EEEEEECCCCCEEEEECCCCCCCCCCCEECCCEEECCCCEEECCCHHHHHHHHHCCEEEC
TFPYTALGRAMTMGQNDGFVKIIAEAKYNRVVGMEIIGAGATEIIHEGVLAIKEEFTLEE
CCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHH
LADAIHAHPTLSESVKEAAEDALGMPINKG
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]