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The map label for this gene is acoA [H]

Identifier: 167040656

GI number: 167040656

Start: 2042633

End: 2043619

Strand: Reverse

Name: acoA [H]

Synonym: Teth514_2033

Alternate gene names: 167040656

Gene position: 2043619-2042633 (Counterclockwise)

Preceding gene: 167040657

Following gene: 167040655

Centisome position: 83.17

GC content: 40.22

Gene sequence:

>987_bases
ATGGAAATTTCAAGAGATATTCTTCTTGATATGTACACTAGAATGGTTAAAATCAGGAAGTTTGAAGAAAAAGTCGCGGA
ACTATTTGCTCAGGGGAAGGTTTTAGGTTTTGTACATTTATACATTGGTGAAGAGGCTACAGCTGTCGGTGTATGCGAAA
ATTTAGAGGATAAAGACTATATTACCAGTACTCACAGAGGTCATGGTCATTTAATAGCAAAAGGTGGCGATCTAAAATAT
ATGATGGCCGAATTATATGGCAAAGAAACGGGATACTGTAAAGGGAAAGGCGGATCTATGCATATAGCGGATGCCACTAA
AGGAATTTTAGGAGCTAATGGTATTGTAGGTGGAGGTTTTCCTATTGCAGCGGGTGCAGCTCTTTCCGCTAAAATGAGAG
GAACTGATCAGGTGGCAGTATGTTTCTTTGGTGATGGCGCCTCCAACCAGAGTACTTTTCACGAGGCGTTGAATATAGCC
TCTATATGGAAGTTACCAGTAGTGTTTGTCTGTGAAAACAACCTTTATGGTATATCTATGAGGCAGGATAAGCACCAAGC
TATCAAAGATGTAGCTGACAGAGCTATTGGTTATGGTATTCCTGGGGTTACGGTGGATGGAAACGATGTATTGGCAGTAT
ATGAAGTAGCAAAAGAAGCTATAAACAGGGCTAGAAGTGGCGCCGGTCCGACTTTAGTTGAATGTAAAACGTATAGATAT
AGAGGGCATTTTGAAGGGGATCCTACTATTTATAGGTCTAAAGAAGAAGTGGAAGAATGGTTGGCAAAAGATCCAATACT
TCGATTAAGTAAACATATACTCGACAATGATGTGGCAACTGAGAAAGAATTAAAAGATATAGAGGCAAGGATAGTTGAAG
AGGTAGAAGAAGCGGTAAGGTTTGCTGAAGAAAGCCCATATCCAAAAGAAGAGGCAGCGGTAGAGGATGTTTATACAGAT
ATAGTAGAGGAGGTAAGGGTAAGATGA

Upstream 100 bases:

>100_bases
CAGGAATAAAAGAGCCGGAATTTGGAGACAAAGATGACACCATTGAAGTTGCAGTGGCTTGCATAACGGTAAGTATAAAA
TGAGGAGGAGATGAATAGTT

Downstream 100 bases:

>100_bases
GGAATATGACGTATGCAGAGGCTTTAAGAGAAGCTATTTTAAATGAGATGAGAAGAGACCCGGCAGTGTTTTTATTAGGA
GAAGATATTGGGAGATTTGG

Product: pyruvate dehydrogenase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MEISRDILLDMYTRMVKIRKFEEKVAELFAQGKVLGFVHLYIGEEATAVGVCENLEDKDYITSTHRGHGHLIAKGGDLKY
MMAELYGKETGYCKGKGGSMHIADATKGILGANGIVGGGFPIAAGAALSAKMRGTDQVAVCFFGDGASNQSTFHEALNIA
SIWKLPVVFVCENNLYGISMRQDKHQAIKDVADRAIGYGIPGVTVDGNDVLAVYEVAKEAINRARSGAGPTLVECKTYRY
RGHFEGDPTIYRSKEEVEEWLAKDPILRLSKHILDNDVATEKELKDIEARIVEEVEEAVRFAEESPYPKEEAAVEDVYTD
IVEEVRVR

Sequences:

>Translated_328_residues
MEISRDILLDMYTRMVKIRKFEEKVAELFAQGKVLGFVHLYIGEEATAVGVCENLEDKDYITSTHRGHGHLIAKGGDLKY
MMAELYGKETGYCKGKGGSMHIADATKGILGANGIVGGGFPIAAGAALSAKMRGTDQVAVCFFGDGASNQSTFHEALNIA
SIWKLPVVFVCENNLYGISMRQDKHQAIKDVADRAIGYGIPGVTVDGNDVLAVYEVAKEAINRARSGAGPTLVECKTYRY
RGHFEGDPTIYRSKEEVEEWLAKDPILRLSKHILDNDVATEKELKDIEARIVEEVEEAVRFAEESPYPKEEAAVEDVYTD
IVEEVRVR
>Mature_328_residues
MEISRDILLDMYTRMVKIRKFEEKVAELFAQGKVLGFVHLYIGEEATAVGVCENLEDKDYITSTHRGHGHLIAKGGDLKY
MMAELYGKETGYCKGKGGSMHIADATKGILGANGIVGGGFPIAAGAALSAKMRGTDQVAVCFFGDGASNQSTFHEALNIA
SIWKLPVVFVCENNLYGISMRQDKHQAIKDVADRAIGYGIPGVTVDGNDVLAVYEVAKEAINRARSGAGPTLVECKTYRY
RGHFEGDPTIYRSKEEVEEWLAKDPILRLSKHILDNDVATEKELKDIEARIVEEVEEAVRFAEESPYPKEEAAVEDVYTD
IVEEVRVR

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=321, Percent_Identity=42.3676012461059, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI291084742, Length=327, Percent_Identity=40.0611620795107, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4505685, Length=327, Percent_Identity=40.0611620795107, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI291084744, Length=334, Percent_Identity=39.2215568862275, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI291084757, Length=327, Percent_Identity=35.7798165137615, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI11386135, Length=326, Percent_Identity=31.5950920245399, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI258645172, Length=326, Percent_Identity=31.9018404907975, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32564172, Length=327, Percent_Identity=40.0611620795107, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17536047, Length=327, Percent_Identity=40.0611620795107, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI86563355, Length=327, Percent_Identity=31.1926605504587, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI86563357, Length=327, Percent_Identity=31.1926605504587, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321026, Length=331, Percent_Identity=39.8791540785499, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24639740, Length=323, Percent_Identity=39.0092879256966, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24639744, Length=323, Percent_Identity=39.0092879256966, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI28571106, Length=323, Percent_Identity=39.0092879256966, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24639748, Length=323, Percent_Identity=39.0092879256966, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24639746, Length=309, Percent_Identity=39.4822006472492, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI21355903, Length=332, Percent_Identity=28.0120481927711, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36172; Mature: 36172

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISRDILLDMYTRMVKIRKFEEKVAELFAQGKVLGFVHLYIGEEATAVGVCENLEDKDY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEHHHCCCCCCHH
ITSTHRGHGHLIAKGGDLKYMMAELYGKETGYCKGKGGSMHIADATKGILGANGIVGGGF
ECCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCEEEECHHHCCHHCCCCCCCCCC
PIAAGAALSAKMRGTDQVAVCFFGDGASNQSTFHEALNIASIWKLPVVFVCENNLYGISM
CHHCCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEE
RQDKHQAIKDVADRAIGYGIPGVTVDGNDVLAVYEVAKEAINRARSGAGPTLVECKTYRY
CCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
RGHFEGDPTIYRSKEEVEEWLAKDPILRLSKHILDNDVATEKELKDIEARIVEEVEEAVR
CCCCCCCCCEECCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FAEESPYPKEEAAVEDVYTDIVEEVRVR
HHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEISRDILLDMYTRMVKIRKFEEKVAELFAQGKVLGFVHLYIGEEATAVGVCENLEDKDY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEHHHCCCCCCHH
ITSTHRGHGHLIAKGGDLKYMMAELYGKETGYCKGKGGSMHIADATKGILGANGIVGGGF
ECCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCEEEECHHHCCHHCCCCCCCCCC
PIAAGAALSAKMRGTDQVAVCFFGDGASNQSTFHEALNIASIWKLPVVFVCENNLYGISM
CHHCCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEE
RQDKHQAIKDVADRAIGYGIPGVTVDGNDVLAVYEVAKEAINRARSGAGPTLVECKTYRY
CCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
RGHFEGDPTIYRSKEEVEEWLAKDPILRLSKHILDNDVATEKELKDIEARIVEEVEEAVR
CCCCCCCCCEECCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FAEESPYPKEEAAVEDVYTDIVEEVRVR
HHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]