Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 167040634

GI number: 167040634

Start: 2019270

End: 2020634

Strand: Reverse

Name: murD [H]

Synonym: Teth514_2011

Alternate gene names: 167040634

Gene position: 2020634-2019270 (Counterclockwise)

Preceding gene: 167040635

Following gene: 167040633

Centisome position: 82.23

GC content: 32.6

Gene sequence:

>1365_bases
ATGGAACTAAAGGGTAAAAAAGTTCTTGTGGCAGGTTTAGGAGTTAGCGGAATAGCTTTGTGTAAAGTGTTGGACAGCTT
GAAGGCGAAAGTAATAGCTTATGATGAAAAAGAATATGATGTATTAAAAGAAAATTTAGAAGAAATTAAAAGTTTGTCCA
TAGATTTTAGGTTTGGTAAATTCAAAAAGGAATTTTTAGAAGGTGTGGATTTGATTGTTTTAAGTCCAGGTGTTCCTATT
GATTCGGATATAGTAAAAACAGCACAAGAAAAGAAAATTGAATTATTGGGAGAAGTGGAGTTTGCATATAGGTTTTCTAA
AGCTCCTATTTATGCAATTACAGGTACAAATGGCAAGACAACTACTACTTCTTTATTAGGAGAAATGTTTAAAAATACGG
GGAGAAAAGTATATGTTGCTGGGAATATTGGTTATCCACTTATTTATGCTGTCATGGAAGCGGCAGAAGATGATTTTATC
GTAGCAGAGATTAGTAGCTTTCAATTAGAGACTATAAAAGAGTTTAAACCTAAAATAAGCTGTATAATAAATATTACTCC
TGATCATTTAGACAGACATAAAACCTTTGAAAATTATAGGGATATAAAAGGAAGAATTTTTGAAAATCAACGAGAAGATG
AATATACTGTTCTCAATTACGATGACCCTGTTACTTGGAGTCTAAAAAATAAGGCTAAATGCAGAGTTTTTCCTTTTAGT
AGAAAAAGTTTATTAGAAAATGGGGCATATATAAAAGATGGAAGTATTTATATAAGTGTTAATGGAAATGCTGAAAAAAT
TATTGACATAGAGGAAATTTATATTCCTGGAGAACACAATTTAGAGAATGCATTGGCCGCTTCTTCAGTAGCATATCTTT
CAGGGATTAGTGCAGATGTTATTGCAAATACTTTAAAGACTTTTAAAGGTGTAGAACATAGGATTGAGTTTGTTGATGAA
ATAAATGGTGTAAAATTTTATAACGATTCTAAAGGGACTAACCCAGACGCTTCAATTAAAGCAATACAGGCCTTAAAAAC
CCCTATAGTTTTAATTGCTGGTGGTTATGATAAAGGTAGTGAATTTGATGAGTTTGTAAAAGCCTTTAATGGGAAAGTAA
AGAAATTGATTTTGATAGGGCAAACTGCAAAAAAAATCAGAGATACTGCAAGGAAGTATTCTTATCCTGAAGATGACATA
CTTTTTGCTGGTACCTTAGAAGAAGCTGTTAAAAAAGCTTATGAAAGTGCGAAAGAGGGAGATAGTGTACTTTTATCACC
AGCTTGTGCCAGTTGGGATATGTTTAGGAATTTTGAGGAGAGGGGAAGAATCTTTAAAAAGGCAGTGGCAGAGTTGAGGA
GGTAA

Upstream 100 bases:

>100_bases
GTCAGGATGGCAAGAACAAAATGTAGTCTACGCTTTTTGGATTGTTACTTTAATTGCGATGTTTTTATCTTTTTATAGTT
TAAGCTAAGGAGTGCAAATT

Downstream 100 bases:

>100_bases
AATAATGAAAAAAAAGTACCCTGTGGATTATGGCATTTTACTCACAGTTTTAATACTAGTCTCCATTGGTGTAATTATGG
TCTTTAGTGCTAGTTCTGCC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MELKGKKVLVAGLGVSGIALCKVLDSLKAKVIAYDEKEYDVLKENLEEIKSLSIDFRFGKFKKEFLEGVDLIVLSPGVPI
DSDIVKTAQEKKIELLGEVEFAYRFSKAPIYAITGTNGKTTTTSLLGEMFKNTGRKVYVAGNIGYPLIYAVMEAAEDDFI
VAEISSFQLETIKEFKPKISCIINITPDHLDRHKTFENYRDIKGRIFENQREDEYTVLNYDDPVTWSLKNKAKCRVFPFS
RKSLLENGAYIKDGSIYISVNGNAEKIIDIEEIYIPGEHNLENALAASSVAYLSGISADVIANTLKTFKGVEHRIEFVDE
INGVKFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGSEFDEFVKAFNGKVKKLILIGQTAKKIRDTARKYSYPEDDI
LFAGTLEEAVKKAYESAKEGDSVLLSPACASWDMFRNFEERGRIFKKAVAELRR

Sequences:

>Translated_454_residues
MELKGKKVLVAGLGVSGIALCKVLDSLKAKVIAYDEKEYDVLKENLEEIKSLSIDFRFGKFKKEFLEGVDLIVLSPGVPI
DSDIVKTAQEKKIELLGEVEFAYRFSKAPIYAITGTNGKTTTTSLLGEMFKNTGRKVYVAGNIGYPLIYAVMEAAEDDFI
VAEISSFQLETIKEFKPKISCIINITPDHLDRHKTFENYRDIKGRIFENQREDEYTVLNYDDPVTWSLKNKAKCRVFPFS
RKSLLENGAYIKDGSIYISVNGNAEKIIDIEEIYIPGEHNLENALAASSVAYLSGISADVIANTLKTFKGVEHRIEFVDE
INGVKFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGSEFDEFVKAFNGKVKKLILIGQTAKKIRDTARKYSYPEDDI
LFAGTLEEAVKKAYESAKEGDSVLLSPACASWDMFRNFEERGRIFKKAVAELRR
>Mature_454_residues
MELKGKKVLVAGLGVSGIALCKVLDSLKAKVIAYDEKEYDVLKENLEEIKSLSIDFRFGKFKKEFLEGVDLIVLSPGVPI
DSDIVKTAQEKKIELLGEVEFAYRFSKAPIYAITGTNGKTTTTSLLGEMFKNTGRKVYVAGNIGYPLIYAVMEAAEDDFI
VAEISSFQLETIKEFKPKISCIINITPDHLDRHKTFENYRDIKGRIFENQREDEYTVLNYDDPVTWSLKNKAKCRVFPFS
RKSLLENGAYIKDGSIYISVNGNAEKIIDIEEIYIPGEHNLENALAASSVAYLSGISADVIANTLKTFKGVEHRIEFVDE
INGVKFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGSEFDEFVKAFNGKVKKLILIGQTAKKIRDTARKYSYPEDDI
LFAGTLEEAVKKAYESAKEGDSVLLSPACASWDMFRNFEERGRIFKKAVAELRR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=453, Percent_Identity=34.2163355408389, Blast_Score=234, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50806; Mature: 50806

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKGKKVLVAGLGVSGIALCKVLDSLKAKVIAYDEKEYDVLKENLEEIKSLSIDFRFGK
CCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCEEEEEEHH
FKKEFLEGVDLIVLSPGVPIDSDIVKTAQEKKIELLGEVEFAYRFSKAPIYAITGTNGKT
HHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHEEECCCCEEEEECCCCCC
TTTSLLGEMFKNTGRKVYVAGNIGYPLIYAVMEAAEDDFIVAEISSFQLETIKEFKPKIS
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEE
CIINITPDHLDRHKTFENYRDIKGRIFENQREDEYTVLNYDDPVTWSLKNKAKCRVFPFS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCEEEEECCH
RKSLLENGAYIKDGSIYISVNGNAEKIIDIEEIYIPGEHNLENALAASSVAYLSGISADV
HHHHHHCCCEEECCEEEEEECCCCCEEEEEEHEEECCCCCHHHHHHHHHHHHHHCCCHHH
IANTLKTFKGVEHRIEFVDEINGVKFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGS
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCC
EFDEFVKAFNGKVKKLILIGQTAKKIRDTARKYSYPEDDILFAGTLEEAVKKAYESAKEG
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCC
DSVLLSPACASWDMFRNFEERGRIFKKAVAELRR
CCEEEECCCCCHHHHHCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELKGKKVLVAGLGVSGIALCKVLDSLKAKVIAYDEKEYDVLKENLEEIKSLSIDFRFGK
CCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCEEEEEEHH
FKKEFLEGVDLIVLSPGVPIDSDIVKTAQEKKIELLGEVEFAYRFSKAPIYAITGTNGKT
HHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHEEECCCCEEEEECCCCCC
TTTSLLGEMFKNTGRKVYVAGNIGYPLIYAVMEAAEDDFIVAEISSFQLETIKEFKPKIS
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEE
CIINITPDHLDRHKTFENYRDIKGRIFENQREDEYTVLNYDDPVTWSLKNKAKCRVFPFS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCEEEEECCH
RKSLLENGAYIKDGSIYISVNGNAEKIIDIEEIYIPGEHNLENALAASSVAYLSGISADV
HHHHHHCCCEEECCEEEEEECCCCCEEEEEEHEEECCCCCHHHHHHHHHHHHHHCCCHHH
IANTLKTFKGVEHRIEFVDEINGVKFYNDSKGTNPDASIKAIQALKTPIVLIAGGYDKGS
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCC
EFDEFVKAFNGKVKKLILIGQTAKKIRDTARKYSYPEDDILFAGTLEEAVKKAYESAKEG
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCC
DSVLLSPACASWDMFRNFEERGRIFKKAVAELRR
CCEEEECCCCCHHHHHCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA