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The map label for this gene is pduD [H]

Identifier: 167040581

GI number: 167040581

Start: 1960969

End: 1961658

Strand: Reverse

Name: pduD [H]

Synonym: Teth514_1952

Alternate gene names: 167040581

Gene position: 1961658-1960969 (Counterclockwise)

Preceding gene: 167040582

Following gene: 167040580

Centisome position: 79.83

GC content: 38.41

Gene sequence:

>690_bases
ATGGTTAAAACAGAATCATTGGTTGAGCAAATTGTAAAGGAAGTTTTGAAAAAGTTGGAAAATGTTGAAATTGCAGCTCC
AGCGACACAATCATCAGATGATGCAAATCAAGAATGGGAAATGATAATTGAGGAGATAGGGGAAGCAAAACAAGGTGTAA
ATGTGGATGAGGTTGTAATAGGGGTTTCTCCTGGCTTTTATATAAAATTTAAGAAAAATATCATTGGTATACCACTGGGT
AATATCCTTCGCGAAATAATATCAGGAATTACAGAACAAGGATTAAAAGCACGAATAGTAAGAGTGAAACACACAGCTGA
TGTTGGGTTCATTGCCCACACAGCTGCAAAACTTTCGGGGTCGGGAATTGGAATTGGTATTCAATCACGAGGTACGGTTG
TTATTCATCAGAAAGACTTACAACCTTTGAACAACCTAGAACTTTTCCCACAATGTCCAGTTTTGACTCTAGAAACGTAT
AGAGCAATTGGAAGAAATGCTGCACTGTATGCCAAAGGCGAATCTCCTACACCAGTTCCTGTACAAAACGATCAAATGGC
AAGGCCTAAATACCAGGCAATAGCTGCAGTAATGCATAATTTTGAAACCAAATATGTGCAAACTGGTGCTAAACCAGTAG
AGTTAAAGGTGTCTTTTGCTAGAAAAGGGGGGAATAAGAGTGATAGATGA

Upstream 100 bases:

>100_bases
TTCAAAAGAGAGATGGGAGCGAATCAAGAATGTACCACAAGCGCTTAAGCCAGAAGATCTAGCCTGAAGAGTTTGATAAA
TAAAGGGGGATAGTGTAAGA

Downstream 100 bases:

>100_bases
AAAGACCCTTGAAATAATAGTACGAGAAGTATTAACCAACTTGACAAGTGATAAAGGAACACAAAATCAGCAAAAAACGG
CAAGCTCTTCTTTGCCAAAG

Product: dehydratase, medium subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduD [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MVKTESLVEQIVKEVLKKLENVEIAAPATQSSDDANQEWEMIIEEIGEAKQGVNVDEVVIGVSPGFYIKFKKNIIGIPLG
NILREIISGITEQGLKARIVRVKHTADVGFIAHTAAKLSGSGIGIGIQSRGTVVIHQKDLQPLNNLELFPQCPVLTLETY
RAIGRNAALYAKGESPTPVPVQNDQMARPKYQAIAAVMHNFETKYVQTGAKPVELKVSFARKGGNKSDR

Sequences:

>Translated_229_residues
MVKTESLVEQIVKEVLKKLENVEIAAPATQSSDDANQEWEMIIEEIGEAKQGVNVDEVVIGVSPGFYIKFKKNIIGIPLG
NILREIISGITEQGLKARIVRVKHTADVGFIAHTAAKLSGSGIGIGIQSRGTVVIHQKDLQPLNNLELFPQCPVLTLETY
RAIGRNAALYAKGESPTPVPVQNDQMARPKYQAIAAVMHNFETKYVQTGAKPVELKVSFARKGGNKSDR
>Mature_229_residues
MVKTESLVEQIVKEVLKKLENVEIAAPATQSSDDANQEWEMIIEEIGEAKQGVNVDEVVIGVSPGFYIKFKKNIIGIPLG
NILREIISGITEQGLKARIVRVKHTADVGFIAHTAAKLSGSGIGIGIQSRGTVVIHQKDLQPLNNLELFPQCPVLTLETY
RAIGRNAALYAKGESPTPVPVQNDQMARPKYQAIAAVMHNFETKYVQTGAKPVELKVSFARKGGNKSDR

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase medium subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010254
- InterPro:   IPR003208 [H]

Pfam domain/function: PF02288 Dehydratase_MU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 24983; Mature: 24983

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKTESLVEQIVKEVLKKLENVEIAAPATQSSDDANQEWEMIIEEIGEAKQGVNVDEVVI
CCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEE
GVSPGFYIKFKKNIIGIPLGNILREIISGITEQGLKARIVRVKHTADVGFIAHTAAKLSG
ECCCCEEEEEECCEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCC
SGIGIGIQSRGTVVIHQKDLQPLNNLELFPQCPVLTLETYRAIGRNAALYAKGESPTPVP
CCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHCCCCEEEECCCCCCCCC
VQNDQMARPKYQAIAAVMHNFETKYVQTGAKPVELKVSFARKGGNKSDR
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MVKTESLVEQIVKEVLKKLENVEIAAPATQSSDDANQEWEMIIEEIGEAKQGVNVDEVVI
CCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEE
GVSPGFYIKFKKNIIGIPLGNILREIISGITEQGLKARIVRVKHTADVGFIAHTAAKLSG
ECCCCEEEEEECCEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCC
SGIGIGIQSRGTVVIHQKDLQPLNNLELFPQCPVLTLETYRAIGRNAALYAKGESPTPVP
CCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHCCCCEEEECCCCCCCCC
VQNDQMARPKYQAIAAVMHNFETKYVQTGAKPVELKVSFARKGGNKSDR
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]