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The map label for this gene is glpF [H]

Identifier: 167040569

GI number: 167040569

Start: 1949852

End: 1950655

Strand: Reverse

Name: glpF [H]

Synonym: Teth514_1940

Alternate gene names: 167040569

Gene position: 1950655-1949852 (Counterclockwise)

Preceding gene: 167040571

Following gene: 167040568

Centisome position: 79.38

GC content: 39.43

Gene sequence:

>804_bases
ATGAAAAGAGAAAATTTAAAAGGGGAGCTTTTATCAGAATTTTTAGGAACCTTGTTGTTGATTATGCTAGGTTGTGGAGT
TGTGGCTAATGTTCTTTTTGCACCACGGCTAAATGGTTTTGGAATTTTCAAAACAGGTGCAGGTTACGATTGGAATGTGA
TAGCTTTTGGGTGGGCAATGGCAGTGACGATAGCAGTATACGTAGCAGGAGGCGTAACTGGTGCACATCTTAATCCAGCA
GTAACATTAGCAGCAGTAGTAAGGAGGGGCTTTCCGCTAGGAAAAGCGATAGCATATATGATAGTTCAAGTAGCAGGAGC
ATTCTGCGGTGCTGGTTTAGCATATGTAATATATAGAGGCAATTTTATAAAAGATGGATATATGAATATATTCTATACAG
GAGCTGCTAACGACAGCTATACACTTGCTAATTCGTTCTTAGTCGAGATGATAGCTACTGCAGTCCTGGTATTAGGAATA
TATGCAGTAACTGATGTACAGTATAATATAGGTATGGCAGCAAACCTTTGGCCAGTAGGAGTAGGCTTTATAATACTGAT
GATAGGATTATGTTTAGGTGGGCCAACTGGTTATGCGATTAATCCAGCCCGTGATTTTGGTCCAAGAGTATTTGCAGCAT
TAATAGGAGACAAGGCAGCATTTTCTGGAGCTTATTGGCTACTTGTTCCAATAATAGGACCATTAGTAGGTGGTGTATTG
GGAGCAATTATTTATGATTACTGCATAGCTCCAAATCTGCCGAAGAAAAGAGAAGAATTAGCGGAAAAAGAAAGTGTAAA
TTAG

Upstream 100 bases:

>100_bases
GAATTCCTTTGAAAATATATAGCAGAATCCCTTAATCCATAAGGATTTTGGGATTCTGCAAAGACCTAAATAAATTATCG
TAGGGAGGGAGAAATAATTT

Downstream 100 bases:

>100_bases
AAAAAACCCAAATTTACATTTGATGGAAGATGAGACCGTTTTAAAAGGAGGATTTTTTATGCGGGCGATTGGATTGTTAG
AGTTTAAAGAAATAACGAGA

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKRENLKGELLSEFLGTLLLIMLGCGVVANVLFAPRLNGFGIFKTGAGYDWNVIAFGWAMAVTIAVYVAGGVTGAHLNPA
VTLAAVVRRGFPLGKAIAYMIVQVAGAFCGAGLAYVIYRGNFIKDGYMNIFYTGAANDSYTLANSFLVEMIATAVLVLGI
YAVTDVQYNIGMAANLWPVGVGFIILMIGLCLGGPTGYAINPARDFGPRVFAALIGDKAAFSGAYWLLVPIIGPLVGGVL
GAIIYDYCIAPNLPKKREELAEKESVN

Sequences:

>Translated_267_residues
MKRENLKGELLSEFLGTLLLIMLGCGVVANVLFAPRLNGFGIFKTGAGYDWNVIAFGWAMAVTIAVYVAGGVTGAHLNPA
VTLAAVVRRGFPLGKAIAYMIVQVAGAFCGAGLAYVIYRGNFIKDGYMNIFYTGAANDSYTLANSFLVEMIATAVLVLGI
YAVTDVQYNIGMAANLWPVGVGFIILMIGLCLGGPTGYAINPARDFGPRVFAALIGDKAAFSGAYWLLVPIIGPLVGGVL
GAIIYDYCIAPNLPKKREELAEKESVN
>Mature_267_residues
MKRENLKGELLSEFLGTLLLIMLGCGVVANVLFAPRLNGFGIFKTGAGYDWNVIAFGWAMAVTIAVYVAGGVTGAHLNPA
VTLAAVVRRGFPLGKAIAYMIVQVAGAFCGAGLAYVIYRGNFIKDGYMNIFYTGAANDSYTLANSFLVEMIATAVLVLGI
YAVTDVQYNIGMAANLWPVGVGFIILMIGLCLGGPTGYAINPARDFGPRVFAALIGDKAAFSGAYWLLVPIIGPLVGGVL
GAIIYDYCIAPNLPKKREELAEKESVN

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=273, Percent_Identity=36.2637362637363, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI22538420, Length=280, Percent_Identity=35, Blast_Score=140, Evalue=8e-34,
Organism=Homo sapiens, GI157266307, Length=279, Percent_Identity=35.1254480286738, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4502187, Length=278, Percent_Identity=32.3741007194245, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4502179, Length=255, Percent_Identity=31.3725490196078, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502181, Length=252, Percent_Identity=28.968253968254, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI4755125, Length=252, Percent_Identity=28.968253968254, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI86792455, Length=208, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4502183, Length=230, Percent_Identity=31.304347826087, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI6912506, Length=268, Percent_Identity=29.4776119402985, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI310133356, Length=204, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI310114181, Length=204, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI37694062, Length=214, Percent_Identity=31.3084112149533, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI45446752, Length=225, Percent_Identity=29.7777777777778, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790362, Length=283, Percent_Identity=37.1024734982332, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787101, Length=207, Percent_Identity=28.5024154589372, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994009, Length=260, Percent_Identity=33.0769230769231, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71992966, Length=257, Percent_Identity=33.4630350194552, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17531429, Length=272, Percent_Identity=34.5588235294118, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17531431, Length=272, Percent_Identity=34.5588235294118, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17544068, Length=242, Percent_Identity=32.6446280991736, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=268, Percent_Identity=31.7164179104478, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32564052, Length=268, Percent_Identity=31.7164179104478, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71992961, Length=251, Percent_Identity=30.6772908366534, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=257, Percent_Identity=26.0700389105058, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322985, Length=279, Percent_Identity=30.4659498207885, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=34.7222222222222, Blast_Score=115, Evalue=7e-27,
Organism=Drosophila melanogaster, GI45550503, Length=268, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24762342, Length=257, Percent_Identity=31.9066147859922, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652747, Length=237, Percent_Identity=31.2236286919831, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45551084, Length=237, Percent_Identity=31.2236286919831, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24762344, Length=258, Percent_Identity=31.7829457364341, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24762346, Length=259, Percent_Identity=30.1158301158301, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24762348, Length=259, Percent_Identity=30.1158301158301, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130305, Length=259, Percent_Identity=30.1158301158301, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28222; Mature: 28222

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00211 ABC_TRANSPORTER_1 ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRENLKGELLSEFLGTLLLIMLGCGVVANVLFAPRLNGFGIFKTGAGYDWNVIAFGWAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHH
AVTIAVYVAGGVTGAHLNPAVTLAAVVRRGFPLGKAIAYMIVQVAGAFCGAGLAYVIYRG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEC
NFIKDGYMNIFYTGAANDSYTLANSFLVEMIATAVLVLGIYAVTDVQYNIGMAANLWPVG
CEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCHHHHH
VGFIILMIGLCLGGPTGYAINPARDFGPRVFAALIGDKAAFSGAYWLLVPIIGPLVGGVL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHH
GAIIYDYCIAPNLPKKREELAEKESVN
HHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKRENLKGELLSEFLGTLLLIMLGCGVVANVLFAPRLNGFGIFKTGAGYDWNVIAFGWAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHH
AVTIAVYVAGGVTGAHLNPAVTLAAVVRRGFPLGKAIAYMIVQVAGAFCGAGLAYVIYRG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEC
NFIKDGYMNIFYTGAANDSYTLANSFLVEMIATAVLVLGIYAVTDVQYNIGMAANLWPVG
CEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCHHHHH
VGFIILMIGLCLGGPTGYAINPARDFGPRVFAALIGDKAAFSGAYWLLVPIIGPLVGGVL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHH
GAIIYDYCIAPNLPKKREELAEKESVN
HHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]