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The map label for this gene is dacF [H]

Identifier: 167040194

GI number: 167040194

Start: 1578985

End: 1580130

Strand: Direct

Name: dacF [H]

Synonym: Teth514_1556

Alternate gene names: 167040194

Gene position: 1578985-1580130 (Clockwise)

Preceding gene: 167040193

Following gene: 167040195

Centisome position: 64.26

GC content: 34.47

Gene sequence:

>1146_bases
ATGTTTAAAAGGCTTTTAACATTATTAGTGAGTGTGATACTAGCTATTTCTTTTGTCTTAAATGGCACAGTATATGCTGA
TACTTTAAATTTAAAGTCAAAATCTGCTATTTTGATAGAGACTAATACTGGACAAATTCTTTATGAAAAAGATAGCCATA
AGCCCTTGCCGCCTGCCAGTGTTACCAAAGTAATGACTCTTTTACTGGCGATTGAAGCAATAGATTCTGGTAAAATTAAA
ATAACAGATAAAGTTGTCACAAGTAAACATGCCTTTGATATGGGAGGTACACAAATATATTTAGAAGTAGGAGAGGAAAT
GACAGTAGATGATTTAATGAAGGCTATAGCGATGAATTCGGCAAATGATGCTTCTGTTGCTTTAGCTGAGTATATTGCGG
GTACAGAGGAAAATTTTGTTGAAATGATGAATAAAAGGGCTAAGGAATTGGGAGCAAAAAATACTACTTTTAAAAATGCA
ACTGGACTACCTGAGGAAGGGCACTTGACAAGCGTATATGATATTGCAATGATTTCAAGGGAATTAGTAAAGCACGAGAG
CATATTTAAATATCTAACCAATAAATTAGATTCTTTAAGAAATGGGAAATTTAGTCTCATAAATACTAATAAATTACTAT
GGCGCTATAAAGGAGTAGATGGAATTAAAACAGGATCTACTTCGGAAGCACTTTATTGTATGGCAGCAACAGCTAAAAGG
GGAGATACAAGGCTAATAGCGGTGGTGTTCGGAGCACCTGATTCTGAGACGAGGTTTAGTGAAGCGGCTAAGTTATTAGA
TTATGGATTTGCTAATTATGAAAGCGTAAAAGTAGCTTCAAAAGGGCAAAGTTTTGGCAATGTTAAAGTTTTAAAAGGGA
AAAGAGAGGCTGTTAGTGTTGTGTGCCGCGATGATGAGTATATACTGATTAAAAAAGGAGAAGGTAAAAATATAAAAACA
GAGGTGGAGCTAAAGAATGCTGTATTAGCTCCTCTAGCCAAAGAGGTCCCAATAGGCACTGTAAAAATAACACAGGATGG
CAAGGTTATAAAGAAATTTAACATATATCCTCAGGAAGTAGTAGAAAAAGCGAACTTTTTTGAAAATCTCAATAAAGTTT
TTATTGGTTGGCTAAAATATCAGTAA

Upstream 100 bases:

>100_bases
AAAGAAGTATATAATAAATTTCACCCTCGTGCTTAAAAATATAAAAAGTATAATATTCACCCTCCAGCGCAAAATAAATA
GTGATGAGGAGGGTGATTTT

Downstream 100 bases:

>100_bases
TTTACTTTAAGCACTAAACAGTCGTTTAGTGCTTTTTTATATTTTTTAGAAAAATATCACAAAAAAGGAGGAATTTTAAA
ATTTATATCGAATATAATAG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MFKRLLTLLVSVILAISFVLNGTVYADTLNLKSKSAILIETNTGQILYEKDSHKPLPPASVTKVMTLLLAIEAIDSGKIK
ITDKVVTSKHAFDMGGTQIYLEVGEEMTVDDLMKAIAMNSANDASVALAEYIAGTEENFVEMMNKRAKELGAKNTTFKNA
TGLPEEGHLTSVYDIAMISRELVKHESIFKYLTNKLDSLRNGKFSLINTNKLLWRYKGVDGIKTGSTSEALYCMAATAKR
GDTRLIAVVFGAPDSETRFSEAAKLLDYGFANYESVKVASKGQSFGNVKVLKGKREAVSVVCRDDEYILIKKGEGKNIKT
EVELKNAVLAPLAKEVPIGTVKITQDGKVIKKFNIYPQEVVEKANFFENLNKVFIGWLKYQ

Sequences:

>Translated_381_residues
MFKRLLTLLVSVILAISFVLNGTVYADTLNLKSKSAILIETNTGQILYEKDSHKPLPPASVTKVMTLLLAIEAIDSGKIK
ITDKVVTSKHAFDMGGTQIYLEVGEEMTVDDLMKAIAMNSANDASVALAEYIAGTEENFVEMMNKRAKELGAKNTTFKNA
TGLPEEGHLTSVYDIAMISRELVKHESIFKYLTNKLDSLRNGKFSLINTNKLLWRYKGVDGIKTGSTSEALYCMAATAKR
GDTRLIAVVFGAPDSETRFSEAAKLLDYGFANYESVKVASKGQSFGNVKVLKGKREAVSVVCRDDEYILIKKGEGKNIKT
EVELKNAVLAPLAKEVPIGTVKITQDGKVIKKFNIYPQEVVEKANFFENLNKVFIGWLKYQ
>Mature_381_residues
MFKRLLTLLVSVILAISFVLNGTVYADTLNLKSKSAILIETNTGQILYEKDSHKPLPPASVTKVMTLLLAIEAIDSGKIK
ITDKVVTSKHAFDMGGTQIYLEVGEEMTVDDLMKAIAMNSANDASVALAEYIAGTEENFVEMMNKRAKELGAKNTTFKNA
TGLPEEGHLTSVYDIAMISRELVKHESIFKYLTNKLDSLRNGKFSLINTNKLLWRYKGVDGIKTGSTSEALYCMAATAKR
GDTRLIAVVFGAPDSETRFSEAAKLLDYGFANYESVKVASKGQSFGNVKVLKGKREAVSVVCRDDEYILIKKGEGKNIKT
EVELKNAVLAPLAKEVPIGTVKITQDGKVIKKFNIYPQEVVEKANFFENLNKVFIGWLKYQ

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=359, Percent_Identity=34.8189415041783, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI87082022, Length=352, Percent_Identity=33.5227272727273, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786851, Length=382, Percent_Identity=32.9842931937173, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI87082054, Length=248, Percent_Identity=27.0161290322581, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42063; Mature: 42063

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRLLTLLVSVILAISFVLNGTVYADTLNLKSKSAILIETNTGQILYEKDSHKPLPPAS
CHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCHH
VTKVMTLLLAIEAIDSGKIKITDKVVTSKHAFDMGGTQIYLEVGEEMTVDDLMKAIAMNS
HHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCC
ANDASVALAEYIAGTEENFVEMMNKRAKELGAKNTTFKNATGLPEEGHLTSVYDIAMISR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ELVKHESIFKYLTNKLDSLRNGKFSLINTNKLLWRYKGVDGIKTGSTSEALYCMAATAKR
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEEECCCC
GDTRLIAVVFGAPDSETRFSEAAKLLDYGFANYESVKVASKGQSFGNVKVLKGKREAVSV
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEE
VCRDDEYILIKKGEGKNIKTEVELKNAVLAPLAKEVPIGTVKITQDGKVIKKFNIYPQEV
EEECCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCHHHH
VEKANFFENLNKVFIGWLKYQ
HHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure
MFKRLLTLLVSVILAISFVLNGTVYADTLNLKSKSAILIETNTGQILYEKDSHKPLPPAS
CHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCHH
VTKVMTLLLAIEAIDSGKIKITDKVVTSKHAFDMGGTQIYLEVGEEMTVDDLMKAIAMNS
HHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCC
ANDASVALAEYIAGTEENFVEMMNKRAKELGAKNTTFKNATGLPEEGHLTSVYDIAMISR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ELVKHESIFKYLTNKLDSLRNGKFSLINTNKLLWRYKGVDGIKTGSTSEALYCMAATAKR
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEEECCCC
GDTRLIAVVFGAPDSETRFSEAAKLLDYGFANYESVKVASKGQSFGNVKVLKGKREAVSV
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEE
VCRDDEYILIKKGEGKNIKTEVELKNAVLAPLAKEVPIGTVKITQDGKVIKKFNIYPQEV
EEECCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCHHHH
VEKANFFENLNKVFIGWLKYQ
HHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]