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The map label for this gene is dus1 [H]

Identifier: 167038941

GI number: 167038941

Start: 293364

End: 294407

Strand: Direct

Name: dus1 [H]

Synonym: Teth514_0273

Alternate gene names: 167038941

Gene position: 293364-294407 (Clockwise)

Preceding gene: 167038940

Following gene: 167038942

Centisome position: 11.94

GC content: 34.48

Gene sequence:

>1044_bases
TTGTTGGATAAAAGAAAATATGTTATAGAATATATAAAGGAATTTTACAGTGAAAGGGATGAAGGAATCTTGAAAATAGG
TGATGTAGTTTTAAAAAATAACGTATTTTTATCTCCTATGGCAGGGGTTACAGATAAGCCTTTTAGATTAATTTGTCAAG
AGATGGGTTGTGGTCTTGCATATACAGAAATGGTGAGTGCCAAAGGTCTTTATTATGGCAGTGAAAATACAAAGCTTTTG
ACAGACATCGATGAAGGAGAAAGTGTAGCTTTACAAATATTCGGCTCTGACCCAGACATTATGGGAGAAATAGCAAAAAG
GCTAAATGACAGTAAAGCTTTAATTCTCGATATAAACATGGGATGCCCTACACCTAAAATAGTTAAAAATGGTGATGGAG
CGGCACTCATGCTAAAACCAGAATTGGCAGAGAAAGTGATGGAATCTGTTGTTAAAGCATCAAATAAGCCTGTGACAGTT
AAAATAAGAAAAGGGTGGGATGACAACCATGTAAATGCTGTGGAAATAGCGCAAATTGCTGAAAAGGTTGGCGTTAAAGC
AGTAGCAGTACATGGAAGGACGAGAGAACAATTTTATTCTGGAAAAGCTGACTGGAGTATAATAAAAAAAGTCAAAGAAA
GTGTAAAAATACCTGTTATAGGCAATGGTGATATATTTACACCAGAAGATGCAAAAAGAATGTTGGAGGAAACGGGATGC
GATGCAATTTTAATAGGACGTGGTGCGCAGGGTAATCCCTGGATATTTAAAAGAACTGTACATTTTTTAAATACCGGAGA
ATTACTTCCTGAGCCGACAATACAAGAAAAAATAGAAGTGATTATCAGGCATTTGGAAATGATGATAGAATATAAAGGAG
AACGAACAGGTATTCTTGAAATGAGAAAACATATTGCTTGGTATTTAAAGGGATTATATGGCAGCGCCAAACTCAGGGAA
GCAGTATTTAAAATGGAAAAATATGAGCAAATAAAAGAGTTTTTATTAAATATAAAATGTAAAAGAGATGCTATTATAAA
TTGA

Upstream 100 bases:

>100_bases
AGTATAAGGGCGGATTTGGATATGTATACGCAAAATATACGAAATAAAATGTAAAGAAAGAAAAGCTGTGGGAAACCGCA
GCTTTTCTTTTTAAGAATAT

Downstream 100 bases:

>100_bases
GGAGTATTTTATTTACATTGAGGTGGTAACGTTGTCAATTGAATATATTATAGCACTTTGCGGTATAGGATTTGCAGCTG
CTTTTATTGATTCTATAGCG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLDKRKYVIEYIKEFYSERDEGILKIGDVVLKNNVFLSPMAGVTDKPFRLICQEMGCGLAYTEMVSAKGLYYGSENTKLL
TDIDEGESVALQIFGSDPDIMGEIAKRLNDSKALILDINMGCPTPKIVKNGDGAALMLKPELAEKVMESVVKASNKPVTV
KIRKGWDDNHVNAVEIAQIAEKVGVKAVAVHGRTREQFYSGKADWSIIKKVKESVKIPVIGNGDIFTPEDAKRMLEETGC
DAILIGRGAQGNPWIFKRTVHFLNTGELLPEPTIQEKIEVIIRHLEMMIEYKGERTGILEMRKHIAWYLKGLYGSAKLRE
AVFKMEKYEQIKEFLLNIKCKRDAIIN

Sequences:

>Translated_347_residues
MLDKRKYVIEYIKEFYSERDEGILKIGDVVLKNNVFLSPMAGVTDKPFRLICQEMGCGLAYTEMVSAKGLYYGSENTKLL
TDIDEGESVALQIFGSDPDIMGEIAKRLNDSKALILDINMGCPTPKIVKNGDGAALMLKPELAEKVMESVVKASNKPVTV
KIRKGWDDNHVNAVEIAQIAEKVGVKAVAVHGRTREQFYSGKADWSIIKKVKESVKIPVIGNGDIFTPEDAKRMLEETGC
DAILIGRGAQGNPWIFKRTVHFLNTGELLPEPTIQEKIEVIIRHLEMMIEYKGERTGILEMRKHIAWYLKGLYGSAKLRE
AVFKMEKYEQIKEFLLNIKCKRDAIIN
>Mature_347_residues
MLDKRKYVIEYIKEFYSERDEGILKIGDVVLKNNVFLSPMAGVTDKPFRLICQEMGCGLAYTEMVSAKGLYYGSENTKLL
TDIDEGESVALQIFGSDPDIMGEIAKRLNDSKALILDINMGCPTPKIVKNGDGAALMLKPELAEKVMESVVKASNKPVTV
KIRKGWDDNHVNAVEIAQIAEKVGVKAVAVHGRTREQFYSGKADWSIIKKVKESVKIPVIGNGDIFTPEDAKRMLEETGC
DAILIGRGAQGNPWIFKRTVHFLNTGELLPEPTIQEKIEVIIRHLEMMIEYKGERTGILEMRKHIAWYLKGLYGSAKLRE
AVFKMEKYEQIKEFLLNIKCKRDAIIN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=259, Percent_Identity=31.6602316602317, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI239788462, Length=229, Percent_Identity=33.1877729257642, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI8923374, Length=256, Percent_Identity=29.296875, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=31.0810810810811, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=32.7510917030568, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1789660, Length=313, Percent_Identity=41.2140575079872, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788462, Length=296, Percent_Identity=31.4189189189189, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI145693211, Length=246, Percent_Identity=27.2357723577236, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=246, Percent_Identity=30.4878048780488, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=250, Percent_Identity=31.2, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=293, Percent_Identity=26.962457337884, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=240, Percent_Identity=28.75, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=290, Percent_Identity=26.8965517241379, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323433, Length=306, Percent_Identity=25.1633986928105, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=26.1061946902655, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=292, Percent_Identity=31.5068493150685, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=32.4444444444444, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=32.4444444444444, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=29.6747967479675, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19921524, Length=255, Percent_Identity=26.2745098039216, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39058; Mature: 39058

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKRKYVIEYIKEFYSERDEGILKIGDVVLKNNVFLSPMAGVTDKPFRLICQEMGCGLA
CCCCHHHHHHHHHHHHCCCCCCEEEECCEEEECCEEECCCCCCCCCHHHHHHHHHCCCCH
YTEMVSAKGLYYGSENTKLLTDIDEGESVALQIFGSDPDIMGEIAKRLNDSKALILDINM
HHHHHHHCCEEECCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEC
GCPTPKIVKNGDGAALMLKPELAEKVMESVVKASNKPVTVKIRKGWDDNHVNAVEIAQIA
CCCCCCEEECCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHH
EKVGVKAVAVHGRTREQFYSGKADWSIIKKVKESVKIPVIGNGDIFTPEDAKRMLEETGC
HHHCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHCEEEEECCCCEECCHHHHHHHHHCCC
DAILIGRGAQGNPWIFKRTVHFLNTGELLPEPTIQEKIEVIIRHLEMMIEYKGERTGILE
CEEEEECCCCCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
MRKHIAWYLKGLYGSAKLREAVFKMEKYEQIKEFLLNIKCKRDAIIN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLDKRKYVIEYIKEFYSERDEGILKIGDVVLKNNVFLSPMAGVTDKPFRLICQEMGCGLA
CCCCHHHHHHHHHHHHCCCCCCEEEECCEEEECCEEECCCCCCCCCHHHHHHHHHCCCCH
YTEMVSAKGLYYGSENTKLLTDIDEGESVALQIFGSDPDIMGEIAKRLNDSKALILDINM
HHHHHHHCCEEECCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEC
GCPTPKIVKNGDGAALMLKPELAEKVMESVVKASNKPVTVKIRKGWDDNHVNAVEIAQIA
CCCCCCEEECCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHH
EKVGVKAVAVHGRTREQFYSGKADWSIIKKVKESVKIPVIGNGDIFTPEDAKRMLEETGC
HHHCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHCEEEEECCCCEECCHHHHHHHHHCCC
DAILIGRGAQGNPWIFKRTVHFLNTGELLPEPTIQEKIEVIIRHLEMMIEYKGERTGILE
CEEEEECCCCCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
MRKHIAWYLKGLYGSAKLREAVFKMEKYEQIKEFLLNIKCKRDAIIN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]