Click here to switch to the map view.

The map label for this gene is mtlF [H]

Identifier: 167038938

GI number: 167038938

Start: 287480

End: 287920

Strand: Direct

Name: mtlF [H]

Synonym: Teth514_0270

Alternate gene names: 167038938

Gene position: 287480-287920 (Clockwise)

Preceding gene: 167038937

Following gene: 167038939

Centisome position: 11.7

GC content: 29.93

Gene sequence:

>441_bases
ATGAATAAAGAAATACTAAACGAAAATAATATCGTATTAAATGTACCATCGGAGTCTAAAGTACAAGCTATTGAAAGAGT
AGGAAATTTGCTTTTTAAAAATGGCTATGTTGAAAAAGAATATATAGAAGGTATGAAAAAAAGAGAAGAAGATGTAACAA
CTTATATAGGTAATGGTATTGCTATTCCTCACGGTGTTTCTGGGTATGTAAAATATATAAAAAAATCTGGAATAGTTATA
GCGCAATATCCTAAAGGAGTAGATTTTGGAGATGGAAATATTGCCTATATTGTCATAGGAATTGCAGGCAAGGGAGATGA
ACATTTAGAGATATTATCAAAAATAGCTCTTACCTGCCAGTATGAAGAAAATGTCGAGAAATTGAAAAAGGCAAGGACAA
AACAAGAAATAATAGAAATACTTCAAAAAGGTGATGAATAA

Upstream 100 bases:

>100_bases
GAGGATAAAGCCTTTAATGAAATATTAAGGTTTGGTGATATCAAAGATATTAGAAATAGCTTAGTTAAAATACTAAATGA
TACACAGGAGGCTTTGGAGG

Downstream 100 bases:

>100_bases
TATGTTAAAAGCGGTTCATTTTGGAGCAGGGAATATAGGCAGAGGATTTATAGGGTATTTGCTTTATAAATCTGGATATG
AAATAACTTTTGTTGATATA

Product: phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MNKEILNENNIVLNVPSESKVQAIERVGNLLFKNGYVEKEYIEGMKKREEDVTTYIGNGIAIPHGVSGYVKYIKKSGIVI
AQYPKGVDFGDGNIAYIVIGIAGKGDEHLEILSKIALTCQYEENVEKLKKARTKQEIIEILQKGDE

Sequences:

>Translated_146_residues
MNKEILNENNIVLNVPSESKVQAIERVGNLLFKNGYVEKEYIEGMKKREEDVTTYIGNGIAIPHGVSGYVKYIKKSGIVI
AQYPKGVDFGDGNIAYIVIGIAGKGDEHLEILSKIALTCQYEENVEKLKKARTKQEIIEILQKGDE
>Mature_146_residues
MNKEILNENNIVLNVPSESKVQAIERVGNLLFKNGYVEKEYIEGMKKREEDVTTYIGNGIAIPHGVSGYVKYIKKSGIVI
AQYPKGVDFGDGNIAYIVIGIAGKGDEHLEILSKIALTCQYEENVEKLKKARTKQEIIEILQKGDE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=122, Percent_Identity=40.1639344262295, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1790027, Length=138, Percent_Identity=37.6811594202899, Blast_Score=102, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16300; Mature: 16300

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILNENNIVLNVPSESKVQAIERVGNLLFKNGYVEKEYIEGMKKREEDVTTYIGNGI
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCE
AIPHGVSGYVKYIKKSGIVIAQYPKGVDFGDGNIAYIVIGIAGKGDEHLEILSKIALTCQ
ECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCC
YEENVEKLKKARTKQEIIEILQKGDE
CHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKEILNENNIVLNVPSESKVQAIERVGNLLFKNGYVEKEYIEGMKKREEDVTTYIGNGI
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCE
AIPHGVSGYVKYIKKSGIVIAQYPKGVDFGDGNIAYIVIGIAGKGDEHLEILSKIALTCQ
ECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCC
YEENVEKLKKARTKQEIIEILQKGDE
CHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8824601 [H]