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The map label for this gene is dctA1

Identifier: 16519987

GI number: 16519987

Start: 146495

End: 148009

Strand: Reverse

Name: dctA1

Synonym: NGR_a01180

Alternate gene names: 16519987

Gene position: 148009-146495 (Counterclockwise)

Preceding gene: 16519986

Following gene: 16519988

Centisome position: 27.61

GC content: 61.19

Gene sequence:

>1515_bases
ATGCGCGGATTACGAGTGTGCATGCACCAAGTGGAGGAAATCATCTTGATCGTAGAAAACTTGGCGGAGGTCCGCGGCAA
GACACCCCATTATAGACATCTATACGTCCAGGTCCTCGCGGCGATCGCCGTGGGCATCCTGCTCGGGTATTTCTATCCGG
ATGTCGGCTCCAAGATGAAGCCGCTCGGCGACGCCTTCATCATGCTCGTCAAGATGATCATCGCGCCGGTGATCTTCCTG
ACGGTCGCGACCGGCATTGCCGGCATGACCGATCTCGCCAAGGTAGGCCGTGTCGCCGGCAAGGCGATGATCTACTTCCT
GACCTTTTCCACCCTCGCGCTCCTCGTCGGCCTCGTGGTCGCCAATGTCGTGCAGCCGGGTGCCGGCATGCACATCGACC
CGGCTTCGCTCGATGCAAAGGCGATCGCCACCTATGCGGAAAAGGCGCATGAGCAGTCGGTCACCGGCTTCCTCATGAAC
ATCATCCCGACGACGCTTGTCGGCGCCTTTGCCGAGGGCGACATCCTCCAGGTGCTGTTCATCTCGGTGCTGTTCGGCAT
CTCGCTCGCGATCGTCGGCAAGAAGGCCGAGGCCGTCGTCGATTTCCTGCACGCGCTGACGTTGCCGATCTTCCGGCTGG
TGGCAATCCTGATGAAGGCCGCCCCGATCGGCGCCTTCGGTGCTATGGCGTTCACCATCGGCAAGTACGGCGTGGCATCC
ATTGCCAATCTCGCGATGCTGATCGGCACCTTCTATCTCACCTCGTTCCTGTTCGTCTTCATGGTGCTCGGCGCGGTCGC
ACGCTACAACGGCTTCTCGATCGTCGCGCTCATCCGCTACATCAAGGAAGAACTGCTGCTCGTGCTCGGGACGTCCTCCT
CGGAAGCGGCGCTCCCGGGGCTGATGAACAAGATGGAGAAGGCAGGCTGCAAGCGCTCGGTCGTCGGCCTCGTCATTCCG
ACCGGCTACTCCTTCAACCTGGACGGCACCAACATCTACATGACGCTCGCGGCGCTGTTCATCGCCCAGGCGACCGATAC
GCCAATCTCCTACGGCGATCAGATCCTGCTGCTCCTCATCGCCATGCTGAGTTCGAAGGGGGCAGCTGGCATCACCGGCG
CTGGCTTCATCACGCTTGCCGCAACCCTCTCCGCGGTTCCCTCCGTGCCGGTCGCCGGCATGGCGCTGATCCTCGGCATC
GACCGCTTCATGTCCGAGTGCCGGGCAATTACCAACATAATCGGCAATGCGGTCGCAACGATTGTGGTGGCGAAGTGGGA
AGGCGAGCTTGCCCCGGCGCAGCTTGCAACCACCCTTGCAGGCAAGGCGCCGGTGGAGACCATGTCGGGGTTGTCAAGCC
AGCGGAGTGACACTGTTGAACTCGGACAAAAAGTGCTGTTTGGTGCAACCAATTCCGCAGATCGTACTCTTGCCGGTCGC
CCAGGGGGGCGCGATTCCCGTCGAATTGCTCCCGATCATTCCGCTCAGGTCTTCGGCGGTCCGCTAAGCTTATGA

Upstream 100 bases:

>100_bases
GAAAGTGCTTCACCAGTCGGGAAAGTTGGCGGAGAGGCTTGAGCTCAAGCGCCGCCTAGGTCGGCTATAGACGGCTGGGA
GGCCTTTTCGTCATCTCTGC

Downstream 100 bases:

>100_bases
GTGACTTAAGGAGAAAGCGAGTGAAGACCAACCCCATCCCGGATCATGTTCCGCCCGCACTCGTGCGGCACTTCAGTCTC
TTCACGTCGCCTGGCATGGC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MRGLRVCMHQVEEIILIVENLAEVRGKTPHYRHLYVQVLAAIAVGILLGYFYPDVGSKMKPLGDAFIMLVKMIIAPVIFL
TVATGIAGMTDLAKVGRVAGKAMIYFLTFSTLALLVGLVVANVVQPGAGMHIDPASLDAKAIATYAEKAHEQSVTGFLMN
IIPTTLVGAFAEGDILQVLFISVLFGISLAIVGKKAEAVVDFLHALTLPIFRLVAILMKAAPIGAFGAMAFTIGKYGVAS
IANLAMLIGTFYLTSFLFVFMVLGAVARYNGFSIVALIRYIKEELLLVLGTSSSEAALPGLMNKMEKAGCKRSVVGLVIP
TGYSFNLDGTNIYMTLAALFIAQATDTPISYGDQILLLLIAMLSSKGAAGITGAGFITLAATLSAVPSVPVAGMALILGI
DRFMSECRAITNIIGNAVATIVVAKWEGELAPAQLATTLAGKAPVETMSGLSSQRSDTVELGQKVLFGATNSADRTLAGR
PGGRDSRRIAPDHSAQVFGGPLSL

Sequences:

>Translated_504_residues
MRGLRVCMHQVEEIILIVENLAEVRGKTPHYRHLYVQVLAAIAVGILLGYFYPDVGSKMKPLGDAFIMLVKMIIAPVIFL
TVATGIAGMTDLAKVGRVAGKAMIYFLTFSTLALLVGLVVANVVQPGAGMHIDPASLDAKAIATYAEKAHEQSVTGFLMN
IIPTTLVGAFAEGDILQVLFISVLFGISLAIVGKKAEAVVDFLHALTLPIFRLVAILMKAAPIGAFGAMAFTIGKYGVAS
IANLAMLIGTFYLTSFLFVFMVLGAVARYNGFSIVALIRYIKEELLLVLGTSSSEAALPGLMNKMEKAGCKRSVVGLVIP
TGYSFNLDGTNIYMTLAALFIAQATDTPISYGDQILLLLIAMLSSKGAAGITGAGFITLAATLSAVPSVPVAGMALILGI
DRFMSECRAITNIIGNAVATIVVAKWEGELAPAQLATTLAGKAPVETMSGLSSQRSDTVELGQKVLFGATNSADRTLAGR
PGGRDSRRIAPDHSAQVFGGPLSL
>Mature_504_residues
MRGLRVCMHQVEEIILIVENLAEVRGKTPHYRHLYVQVLAAIAVGILLGYFYPDVGSKMKPLGDAFIMLVKMIIAPVIFL
TVATGIAGMTDLAKVGRVAGKAMIYFLTFSTLALLVGLVVANVVQPGAGMHIDPASLDAKAIATYAEKAHEQSVTGFLMN
IIPTTLVGAFAEGDILQVLFISVLFGISLAIVGKKAEAVVDFLHALTLPIFRLVAILMKAAPIGAFGAMAFTIGKYGVAS
IANLAMLIGTFYLTSFLFVFMVLGAVARYNGFSIVALIRYIKEELLLVLGTSSSEAALPGLMNKMEKAGCKRSVVGLVIP
TGYSFNLDGTNIYMTLAALFIAQATDTPISYGDQILLLLIAMLSSKGAAGITGAGFITLAATLSAVPSVPVAGMALILGI
DRFMSECRAITNIIGNAVATIVVAKWEGELAPAQLATTLAGKAPVETMSGLSSQRSDTVELGQKVLFGATNSADRTLAGR
PGGRDSRRIAPDHSAQVFGGPLSL

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the inner membrane. This transport system plays an essential role in the energy supply of tropical rhizobium-legume symbionts [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=459, Percent_Identity=25.4901960784314, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI40254478, Length=470, Percent_Identity=25.1063829787234, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI169790839, Length=463, Percent_Identity=26.9978401727862, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194239697, Length=480, Percent_Identity=25.4166666666667, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI66773030, Length=456, Percent_Identity=23.9035087719298, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI21314632, Length=449, Percent_Identity=24.4988864142539, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI5032093, Length=388, Percent_Identity=26.8041237113402, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI223468566, Length=289, Percent_Identity=28.719723183391, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI223468564, Length=259, Percent_Identity=28.957528957529, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI262359914, Length=423, Percent_Identity=25.7683215130024, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI301601644, Length=207, Percent_Identity=27.536231884058, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789947, Length=427, Percent_Identity=59.9531615925059, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1790514, Length=408, Percent_Identity=38.4803921568627, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1788024, Length=394, Percent_Identity=23.8578680203046, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=457, Percent_Identity=25.382932166302, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71983099, Length=448, Percent_Identity=25.2232142857143, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983106, Length=448, Percent_Identity=25.2232142857143, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=446, Percent_Identity=25.3363228699552, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193206505, Length=508, Percent_Identity=24.0157480314961, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=434, Percent_Identity=23.963133640553, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=403, Percent_Identity=25.0620347394541, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583025, Length=404, Percent_Identity=25, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI17137668, Length=404, Percent_Identity=25, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24583023, Length=404, Percent_Identity=25, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI281360483, Length=452, Percent_Identity=24.5575221238938, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137666, Length=452, Percent_Identity=24.5575221238938, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281360481, Length=452, Percent_Identity=24.5575221238938, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 52946; Mature: 52946

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1617199; 8796346; 9163424 [H]