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Identifier: 16519971

GI number: 16519971

Start: 163474

End: 164805

Strand: Reverse

Name: Not Available

Synonym: NGR_a01340

Alternate gene names: 16519971

Gene position: 164805-163474 (Counterclockwise)

Preceding gene: 16519969

Following gene: 16519972

Centisome position: 30.74

GC content: 60.14

Gene sequence:

>1332_bases
ATGTCGTTCCTTGATCTACCGGAGGGGCTGCCAGAACGGATCATCCAGTGCAACTCTCCCTACACGGTCCGCTTCGGTGT
ACGGCTGCGCGGCCGCATGTACAGCTTCATCGGCTGGCGATCGGTGCGCGAGCACTGCGAACCAGTGAAGGGCGACATTC
GATATGCGTCAAATGCTGACGCAGAGGAGGTCGAGGCGCTTGCCGCACTAATGACGCTCAAATGCTCGCTTGTTGACGTG
CCGTTCGGTGGCTCCAAAGGGGCACTCAAGATCGACCCGCGAGGATGGACCCCGCAGGAGCTCGAGCACATCACTCGACG
CTTTACGCAGGAGATGAACAAGCGCCCCGATCGGGCCAGGCGTCAATGTGTCGGCTCCGATATCGGCACCGGTGAGCGGG
AAATGGCCTGGATGATGGATGAGTTCCGCCGCGCCAATCCTACCGACGTGGTCACTTCGGGTGCTTGTGTCACCGGTAAG
CCGCTGTCGAAAGGCGGGATCGCCGGGCGAGCGGAGTCTACGGGCAGGGGCGTTCAATTCGCGATCCAGAGCTCCCTTCG
GGACACCCGCACGCCGGGTTTGGATGGCCGGCGCAATCTCAAAGGCGCCTCCACTGTTATACAGGGGTTTGGCAATGTCG
GCTATCACGCCGCCAGGTTTTTGTCCGAAGAGGATGATGCACGCGTGACCGTTCTTGCTGAACGTGACGGGTATGTCGCC
AATCCGGAAGGCCTCTCCATTGAGGCCCTCAAGCAACATCAGATCCGAACAGGCAGCATTCTCGGCTTTGATGGAGCCAA
GTCCATTGCCGGCGATATGTGCGGCGTCGAACAGCCCTGCGACGTTCTGATTCCAGCGGCGATGGAAAATGCTATTCATG
CTGAAAACGCGGAGCGCATGAAGGCACATCTCGTCGTCGAAGCCGCCAACGGACCTGTCACCTTCGAAGCGGACGAGATC
CTTCGCTCCCGCGGCGTGACCATTCTTCCCGATCTTTATGTTAATGCCGGAGGTGTCGTCGTCAGTTACTTCGAGCGGGT
GAAGAACCTCACGCACATCCCCTTCGGGCTCATGGAGCGGCGGCGGCGGGAACGGGGCAACCACACAATCGCTACGGCGC
TTGAACGGATGACAGGCAAGGAGTCCCCCGCAGACATGCGCGACGAATTCCTCGAAGGCGGCGCCGAAATCGACCTCGTT
CGTTCCGGCCTGGAAGACGTCATGCGCAGCACCTGGACGCGCATTGCCGACCTGATGGAACAGCAACCGGAACTCGGTGA
CTACAGAACTGCCGCCTATGTCGCGTCTATCCGGCAGGTCGCTGATTTATGA

Upstream 100 bases:

>100_bases
ACAATCTCGACGCAACGCTCCTCAAGCGGTTACAGGTGCGTGATGCAAACGAATTCTGCTGGCTTTCTTGAAAGCGTAGA
CCCAAAACTTCCGCCATGCC

Downstream 100 bases:

>100_bases
GGCGATTGGGATATGACCGCGTATGGCGAAAACAGCGTGCGGCGCTACCGACTACGCTTACTCTCACGCATGGCTCCACT
CCCGGCGATGCATGCCCAAG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSFLDLPEGLPERIIQCNSPYTVRFGVRLRGRMYSFIGWRSVREHCEPVKGDIRYASNADAEEVEALAALMTLKCSLVDV
PFGGSKGALKIDPRGWTPQELEHITRRFTQEMNKRPDRARRQCVGSDIGTGEREMAWMMDEFRRANPTDVVTSGACVTGK
PLSKGGIAGRAESTGRGVQFAIQSSLRDTRTPGLDGRRNLKGASTVIQGFGNVGYHAARFLSEEDDARVTVLAERDGYVA
NPEGLSIEALKQHQIRTGSILGFDGAKSIAGDMCGVEQPCDVLIPAAMENAIHAENAERMKAHLVVEAANGPVTFEADEI
LRSRGVTILPDLYVNAGGVVVSYFERVKNLTHIPFGLMERRRRERGNHTIATALERMTGKESPADMRDEFLEGGAEIDLV
RSGLEDVMRSTWTRIADLMEQQPELGDYRTAAYVASIRQVADL

Sequences:

>Translated_443_residues
MSFLDLPEGLPERIIQCNSPYTVRFGVRLRGRMYSFIGWRSVREHCEPVKGDIRYASNADAEEVEALAALMTLKCSLVDV
PFGGSKGALKIDPRGWTPQELEHITRRFTQEMNKRPDRARRQCVGSDIGTGEREMAWMMDEFRRANPTDVVTSGACVTGK
PLSKGGIAGRAESTGRGVQFAIQSSLRDTRTPGLDGRRNLKGASTVIQGFGNVGYHAARFLSEEDDARVTVLAERDGYVA
NPEGLSIEALKQHQIRTGSILGFDGAKSIAGDMCGVEQPCDVLIPAAMENAIHAENAERMKAHLVVEAANGPVTFEADEI
LRSRGVTILPDLYVNAGGVVVSYFERVKNLTHIPFGLMERRRRERGNHTIATALERMTGKESPADMRDEFLEGGAEIDLV
RSGLEDVMRSTWTRIADLMEQQPELGDYRTAAYVASIRQVADL
>Mature_442_residues
SFLDLPEGLPERIIQCNSPYTVRFGVRLRGRMYSFIGWRSVREHCEPVKGDIRYASNADAEEVEALAALMTLKCSLVDVP
FGGSKGALKIDPRGWTPQELEHITRRFTQEMNKRPDRARRQCVGSDIGTGEREMAWMMDEFRRANPTDVVTSGACVTGKP
LSKGGIAGRAESTGRGVQFAIQSSLRDTRTPGLDGRRNLKGASTVIQGFGNVGYHAARFLSEEDDARVTVLAERDGYVAN
PEGLSIEALKQHQIRTGSILGFDGAKSIAGDMCGVEQPCDVLIPAAMENAIHAENAERMKAHLVVEAANGPVTFEADEIL
RSRGVTILPDLYVNAGGVVVSYFERVKNLTHIPFGLMERRRRERGNHTIATALERMTGKESPADMRDEFLEGGAEIDLVR
SGLEDVMRSTWTRIADLMEQQPELGDYRTAAYVASIRQVADL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI4885281, Length=449, Percent_Identity=40.7572383073497, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI31377775, Length=449, Percent_Identity=39.6436525612472, Blast_Score=304, Evalue=1e-82,
Organism=Escherichia coli, GI1788059, Length=352, Percent_Identity=28.9772727272727, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17544676, Length=452, Percent_Identity=39.1592920353982, Blast_Score=286, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6324951, Length=371, Percent_Identity=25.3369272237197, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319256, Length=374, Percent_Identity=24.5989304812834, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45553475, Length=451, Percent_Identity=39.2461197339246, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI45549226, Length=464, Percent_Identity=38.3620689655172, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24649460, Length=408, Percent_Identity=38.9705882352941, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24649283, Length=433, Percent_Identity=37.1824480369515, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DHE3_RHISN (Q53199)

Other databases:

- EMBL:   Z68203
- EMBL:   U00090
- RefSeq:   NP_444091.1
- ProteinModelPortal:   Q53199
- SMR:   Q53199
- GeneID:   962463
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01340
- HOGENOM:   HBG590661
- ProtClustDB:   CLSK2461523
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11606:SF2
- PIRSF:   PIRSF000185
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.3

Molecular weight: Translated: 48846; Mature: 48715

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 86-86

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLDLPEGLPERIIQCNSPYTVRFGVRLRGRMYSFIGWRSVREHCEPVKGDIRYASNAD
CCCCCCCCCCHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCC
AEEVEALAALMTLKCSLVDVPFGGSKGALKIDPRGWTPQELEHITRRFTQEMNKRPDRAR
HHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
RQCVGSDIGTGEREMAWMMDEFRRANPTDVVTSGACVTGKPLSKGGIAGRAESTGRGVQF
HHHCCCCCCCCHHHHHHHHHHHHCCCCCHHEECCCEECCCCCCCCCCCCCCCCCCCCCHH
AIQSSLRDTRTPGLDGRRNLKGASTVIQGFGNVGYHAARFLSEEDDARVTVLAERDGYVA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEECCCEEE
NPEGLSIEALKQHQIRTGSILGFDGAKSIAGDMCGVEQPCDVLIPAAMENAIHAENAERM
CCCCCCHHHHHHCCCCCCCEECCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHHHCCHHHH
KAHLVVEAANGPVTFEADEILRSRGVTILPDLYVNAGGVVVSYFERVKNLTHIPFGLMER
EEEEEEEECCCCEEECHHHHHHHCCCEEECHHHCCCCHHHHHHHHHHHCCCCCCHHHHHH
RRRERGNHTIATALERMTGKESPADMRDEFLEGGAEIDLVRSGLEDVMRSTWTRIADLME
HHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQPELGDYRTAAYVASIRQVADL
CCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFLDLPEGLPERIIQCNSPYTVRFGVRLRGRMYSFIGWRSVREHCEPVKGDIRYASNAD
CCCCCCCCCHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCC
AEEVEALAALMTLKCSLVDVPFGGSKGALKIDPRGWTPQELEHITRRFTQEMNKRPDRAR
HHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
RQCVGSDIGTGEREMAWMMDEFRRANPTDVVTSGACVTGKPLSKGGIAGRAESTGRGVQF
HHHCCCCCCCCHHHHHHHHHHHHCCCCCHHEECCCEECCCCCCCCCCCCCCCCCCCCCHH
AIQSSLRDTRTPGLDGRRNLKGASTVIQGFGNVGYHAARFLSEEDDARVTVLAERDGYVA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEECCCEEE
NPEGLSIEALKQHQIRTGSILGFDGAKSIAGDMCGVEQPCDVLIPAAMENAIHAENAERM
CCCCCCHHHHHHCCCCCCCEECCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHHHCCHHHH
KAHLVVEAANGPVTFEADEILRSRGVTILPDLYVNAGGVVVSYFERVKNLTHIPFGLMER
EEEEEEEECCCCEEECHHHHHHHCCCEEECHHHCCCCHHHHHHHHHHHCCCCCCHHHHHH
RRRERGNHTIATALERMTGKESPADMRDEFLEGGAEIDLVRSGLEDVMRSTWTRIADLME
HHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQPELGDYRTAAYVASIRQVADL
CCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424