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Identifier: 16519679

GI number: 16519679

Start: 6654

End: 7571

Strand: Reverse

Name: Not Available

Synonym: NGR_a00070

Alternate gene names: 16519679

Gene position: 7571-6654 (Counterclockwise)

Preceding gene: 16519672

Following gene: NA

Centisome position: 1.41

GC content: 65.9

Gene sequence:

>918_bases
ATGGCAACGGCTGCATACCTCAATTCTCCTTCCCTGCAGCAAAGGCTGATCGGATACGCCCGTGTTTCGACCGAGGATCA
GCTCAACGACGCTCAGGTCGACGAATTGCGGGCGGCTGGTTGCCACCGCATCCACCAGGAGCACGGATCCGGCGCATCAC
GCGCGCGGCCGGTGCTTGCGAAGCTTCTTAAAGACCTTGCCATGGGCGATGTCCTCGTCGTCGTTCGCCTCGACCGTCTG
GCCCGATCGGTCAGCCACCTGCTCGACGTCATCGAAGACCTCGAGAAGCGCGGCGTCCATTTCCGCTCGCTGCGTGATCC
GATCGATACCTCGACGCCGCACGGAATGTTTTCCCTGCAGGTGCTCGGCGCCGTCGCCCAGCTCGAGCGCGCGCTGATCG
CGGAGCGGACCAAGTCCGGTATGCAGGCCGCCAAGGCGCGCGGCCGGCTTGCCGGCAATCCCGGGCTTCGAGAACGCCGG
CCAGAAGCCATCCGTGCGGTCTCAGCGGCGCGCGAGCGGGCCTACCTCGATGAACTGATTGTGTCAGCGCAGACCTGGCT
GCCGACAGTCCGGCGACTGCGCCCGCGACACAGTTGGGACAATGTCGTGCGGATCCTCAATCGCAGGGGGCACGACTGGA
CCGTCGAACGGTTGCGGCGGGCGGTCCACCGGCTAGTGCGCGAAAAGCTCGCGGAACCGGAACTGCTTGCCCGATCGCTG
CGCCGGCCGCCCGAGGATCATCTGATGCGGCTGGTTGCCGGGATCGCCATCGCCGATCCCAATCTGTCGCTGCGCGACAT
TGCCGCCCAGTTGGACCAGATGCAGGAGCGACCGCCACGTGGCGGCCGCAAATGGCAACCGTCTTCCGTCCGAGCACTAC
TGGACGAGGCGAGTCGCATCGGGCTGGTTCGCGCTTGA

Upstream 100 bases:

>100_bases
GTACGGCCTTCCATTTTGAACATAAAACACGCTTGCAAAGGAGCCTTTTCTCACACATGTTTCATTTGTACAAACCGCCC
CGAAACGACCGAAAAATTCG

Downstream 100 bases:

>100_bases
GAGGCTTGGTTCATGAATTCAAGAGTGTTCACGCGGCGTCGAAAGACGGGTGCGGCACTGTTTCCGGTCGCCGCATGCTT
CCAAATGGAAAAAGACCGAG

Product: DNA integration/recombination/inversion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MATAAYLNSPSLQQRLIGYARVSTEDQLNDAQVDELRAAGCHRIHQEHGSGASRARPVLAKLLKDLAMGDVLVVVRLDRL
ARSVSHLLDVIEDLEKRGVHFRSLRDPIDTSTPHGMFSLQVLGAVAQLERALIAERTKSGMQAAKARGRLAGNPGLRERR
PEAIRAVSAARERAYLDELIVSAQTWLPTVRRLRPRHSWDNVVRILNRRGHDWTVERLRRAVHRLVREKLAEPELLARSL
RRPPEDHLMRLVAGIAIADPNLSLRDIAAQLDQMQERPPRGGRKWQPSSVRALLDEASRIGLVRA

Sequences:

>Translated_305_residues
MATAAYLNSPSLQQRLIGYARVSTEDQLNDAQVDELRAAGCHRIHQEHGSGASRARPVLAKLLKDLAMGDVLVVVRLDRL
ARSVSHLLDVIEDLEKRGVHFRSLRDPIDTSTPHGMFSLQVLGAVAQLERALIAERTKSGMQAAKARGRLAGNPGLRERR
PEAIRAVSAARERAYLDELIVSAQTWLPTVRRLRPRHSWDNVVRILNRRGHDWTVERLRRAVHRLVREKLAEPELLARSL
RRPPEDHLMRLVAGIAIADPNLSLRDIAAQLDQMQERPPRGGRKWQPSSVRALLDEASRIGLVRA
>Mature_304_residues
ATAAYLNSPSLQQRLIGYARVSTEDQLNDAQVDELRAAGCHRIHQEHGSGASRARPVLAKLLKDLAMGDVLVVVRLDRLA
RSVSHLLDVIEDLEKRGVHFRSLRDPIDTSTPHGMFSLQVLGAVAQLERALIAERTKSGMQAAKARGRLAGNPGLRERRP
EAIRAVSAARERAYLDELIVSAQTWLPTVRRLRPRHSWDNVVRILNRRGHDWTVERLRRAVHRLVREKLAEPELLARSLR
RPPEDHLMRLVAGIAIADPNLSLRDIAAQLDQMQERPPRGGRKWQPSSVRALLDEASRIGLVRA

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family

Homologues:

Organism=Escherichia coli, GI1787404, Length=147, Percent_Identity=52.3809523809524, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=35.5191256830601, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=35.5191256830601, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4CG_RHISN (P55389)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443799.1
- ProteinModelPortal:   P55389
- SMR:   P55389
- GeneID:   962300
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a00070
- HOGENOM:   HBG294912
- ProtClustDB:   CLSK861953
- InterPro:   IPR006118
- InterPro:   IPR006119
- Gene3D:   G3DSA:3.40.50.1390
- SMART:   SM00857

Pfam domain/function: PF00239 Resolvase; SSF53041 Resolv_N

EC number: NA

Molecular weight: Translated: 34278; Mature: 34147

Theoretical pI: Translated: 11.60; Mature: 11.60

Prosite motif: PS00397 RECOMBINASES_1; PS00398 RECOMBINASES_2

Important sites: ACT_SITE 23-23

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAAYLNSPSLQQRLIGYARVSTEDQLNDAQVDELRAAGCHRIHQEHGSGASRARPVLA
CCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KLLKDLAMGDVLVVVRLDRLARSVSHLLDVIEDLEKRGVHFRSLRDPIDTSTPHGMFSLQ
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
VLGAVAQLERALIAERTKSGMQAAKARGRLAGNPGLRERRPEAIRAVSAARERAYLDELI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VSAQTWLPTVRRLRPRHSWDNVVRILNRRGHDWTVERLRRAVHRLVREKLAEPELLARSL
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RRPPEDHLMRLVAGIAIADPNLSLRDIAAQLDQMQERPPRGGRKWQPSSVRALLDEASRI
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
GLVRA
CCCCC
>Mature Secondary Structure 
ATAAYLNSPSLQQRLIGYARVSTEDQLNDAQVDELRAAGCHRIHQEHGSGASRARPVLA
CCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KLLKDLAMGDVLVVVRLDRLARSVSHLLDVIEDLEKRGVHFRSLRDPIDTSTPHGMFSLQ
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
VLGAVAQLERALIAERTKSGMQAAKARGRLAGNPGLRERRPEAIRAVSAARERAYLDELI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VSAQTWLPTVRRLRPRHSWDNVVRILNRRGHDWTVERLRRAVHRLVREKLAEPELLARSL
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RRPPEDHLMRLVAGIAIADPNLSLRDIAAQLDQMQERPPRGGRKWQPSSVRALLDEASRI
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
GLVRA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424