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The map label for this gene is noeJ

Identifier: 16519649

GI number: 16519649

Start: 45284

End: 46822

Strand: Reverse

Name: noeJ

Synonym: NGR_a00380

Alternate gene names: 16519649

Gene position: 46822-45284 (Counterclockwise)

Preceding gene: 16520056

Following gene: 16519650

Centisome position: 8.73

GC content: 55.04

Gene sequence:

>1539_bases
ATGAATTCGCATCCACTAATGAGATTGCTTCGCTCTCGCCACACTGTTATGAAGCTTCCATCAAACGCAATCAAAAATGA
CACCATGCTTCCAAAGATTATCCCAGCTATCATGGCAGGGGGTAGGGGCACAAGGCTCTGGCCTCTGTCACGCGCAACTG
CCGCAAAACAGTTTCTAAAGCTAATCGGCGAGGAAACGCTCTTTCAAGACACGCTTAAGCGCGTTTCCGATGCCAAAGTT
TATGGAGCACCACTCGTCATCACAAATGAGGAATTTCGCTTCCTGGTTGCCGAACAGGCGCGCGAGCTCGGGGTCACGCT
TTCCAGCATAGTACTTGAGCCGGTGCCGCGCAACACGGCAGCCGCAGTAGCCGTCGCCGCCCGGATCGTGGCTGATCGGT
TTGGTGAGGACGCGCTATTGCTTGTGCTGCCGTCGGACCACGCGATTACGGTAGACGATACTTACAAGAAATGCGTGCGC
TCCGCCTGCATCGCCGCAGCGGAGGGCAAGCTCGTGACGTTCGGTATTCAACCCACTTGGCCAGCAACAGGATACGGATA
CATCGAACGTGGCACTTACCTTGGCAAGGACGTCCATGCGGTTCAATGTTTTGTCGAGAAACCAAGTCTCGAAAAGGCGG
CGGCTCTTTTGGAGACCGGCAATTACTATTGGAACTCTGGGATGTTCCTTTTCCAGGCGGCCAGCATTATCGCCGAACTT
GAGGAACATGCACCCGACGTGTTGTCGGCGGTGCACGCCGCGGTCAGGGGCTCAACGGTCGACGCCGATTTTATACGACT
TGCCCCGGAGAGTTTCAGCCAAGCGCCATCCATCTCGATCGACTATGCACTGATGGAGAAAACCGCAAACGCCGCCGTGG
TCTGCTCGGATTTTGCTTGGTCGGATCTCGGTAGTTGGGATGCTGTATGGAAGAATGAGGAGCAGAATGCTGATGGTAAC
GTGCTCAAGGGCAATGTTACGGCCTGCAACACGAAGAATTCGCTTGTGTTGTCGCATACCGCGCATCTCGCTGTACAGGG
GATGGATGGAGTTGCAGTCATCGCTAGCGAGGACGCAGTCTTCGTCGGGCGACTGGAGGAGGCCCATGAGATCGGAAACT
TGGTGAAGCGCCTCGCCGCGGACGAAAATACGGCACGTCTTACGGAATTGCACCCAACTTTGATACGGCCATGGGGCGGC
TACACCACTATGCTTAACGGCGATCGCTTCCAAGTAAGGCGGTTGTTCGTACGCCCTGGGAAGATGCTTTCTCTCCACAA
GCATTTTCATCGATCAGAGCACTGGATCTGCGTGAAGGGCACCGCAGAAGTGACAATCGAGGATCGAGTAACGATCCTGC
ACGAGAACCAGTCAATCTACATCCCCGAGGGGGCGATACATCGCTTGGGCAACCCTGGCAAGATCATGCTGGAGTTGGTC
GAGATTCAAACTGGTGCCTACCTGGGCGAGGACGATATCATCCGCGTCGCAGACGAATCGAGAAATGAAATGCCAGATTC
GAGGCGTACGGGCCCATAG

Upstream 100 bases:

>100_bases
AACCCGCGCAGACCACTTGTGCCGAATTTGGGACCCATTAACTAGCCTTTTTTTGCCGGGCAGCGTTCAGCAGGTAGTCG
ACGCCACCGGGGCAAGCCGA

Downstream 100 bases:

>100_bases
GGATAGCGTGCCCTTTGCGCAGTCCGGCACCTTCGCACGCATTACGGTATCTGGAGCGAGGACGTGGTGGCACCATCGGC
AAGCGTTGCACCGGCGGCCG

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MNSHPLMRLLRSRHTVMKLPSNAIKNDTMLPKIIPAIMAGGRGTRLWPLSRATAAKQFLKLIGEETLFQDTLKRVSDAKV
YGAPLVITNEEFRFLVAEQARELGVTLSSIVLEPVPRNTAAAVAVAARIVADRFGEDALLLVLPSDHAITVDDTYKKCVR
SACIAAAEGKLVTFGIQPTWPATGYGYIERGTYLGKDVHAVQCFVEKPSLEKAAALLETGNYYWNSGMFLFQAASIIAEL
EEHAPDVLSAVHAAVRGSTVDADFIRLAPESFSQAPSISIDYALMEKTANAAVVCSDFAWSDLGSWDAVWKNEEQNADGN
VLKGNVTACNTKNSLVLSHTAHLAVQGMDGVAVIASEDAVFVGRLEEAHEIGNLVKRLAADENTARLTELHPTLIRPWGG
YTTMLNGDRFQVRRLFVRPGKMLSLHKHFHRSEHWICVKGTAEVTIEDRVTILHENQSIYIPEGAIHRLGNPGKIMLELV
EIQTGAYLGEDDIIRVADESRNEMPDSRRTGP

Sequences:

>Translated_512_residues
MNSHPLMRLLRSRHTVMKLPSNAIKNDTMLPKIIPAIMAGGRGTRLWPLSRATAAKQFLKLIGEETLFQDTLKRVSDAKV
YGAPLVITNEEFRFLVAEQARELGVTLSSIVLEPVPRNTAAAVAVAARIVADRFGEDALLLVLPSDHAITVDDTYKKCVR
SACIAAAEGKLVTFGIQPTWPATGYGYIERGTYLGKDVHAVQCFVEKPSLEKAAALLETGNYYWNSGMFLFQAASIIAEL
EEHAPDVLSAVHAAVRGSTVDADFIRLAPESFSQAPSISIDYALMEKTANAAVVCSDFAWSDLGSWDAVWKNEEQNADGN
VLKGNVTACNTKNSLVLSHTAHLAVQGMDGVAVIASEDAVFVGRLEEAHEIGNLVKRLAADENTARLTELHPTLIRPWGG
YTTMLNGDRFQVRRLFVRPGKMLSLHKHFHRSEHWICVKGTAEVTIEDRVTILHENQSIYIPEGAIHRLGNPGKIMLELV
EIQTGAYLGEDDIIRVADESRNEMPDSRRTGP
>Mature_512_residues
MNSHPLMRLLRSRHTVMKLPSNAIKNDTMLPKIIPAIMAGGRGTRLWPLSRATAAKQFLKLIGEETLFQDTLKRVSDAKV
YGAPLVITNEEFRFLVAEQARELGVTLSSIVLEPVPRNTAAAVAVAARIVADRFGEDALLLVLPSDHAITVDDTYKKCVR
SACIAAAEGKLVTFGIQPTWPATGYGYIERGTYLGKDVHAVQCFVEKPSLEKAAALLETGNYYWNSGMFLFQAASIIAEL
EEHAPDVLSAVHAAVRGSTVDADFIRLAPESFSQAPSISIDYALMEKTANAAVVCSDFAWSDLGSWDAVWKNEEQNADGN
VLKGNVTACNTKNSLVLSHTAHLAVQGMDGVAVIASEDAVFVGRLEEAHEIGNLVKRLAADENTARLTELHPTLIRPWGG
YTTMLNGDRFQVRRLFVRPGKMLSLHKHFHRSEHWICVKGTAEVTIEDRVTILHENQSIYIPEGAIHRLGNPGKIMLELV
EIQTGAYLGEDDIIRVADESRNEMPDSRRTGP

Specific function: Involved In The Biosynthesis Of The Capsular Polysaccharide Colanic Acid. [C]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family

Homologues:

Organism=Escherichia coli, GI1788362, Length=472, Percent_Identity=45.3389830508475, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NOEJ_RHISN (P55357)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443769.1
- ProteinModelPortal:   P55357
- SMR:   P55357
- GeneID:   962255
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a00380
- HOGENOM:   HBG422488
- ProtClustDB:   CLSK893845
- GO:   GO:0009877
- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- TIGRFAMs:   TIGR01479

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase; SSF51182 RmlC_like_cupin

EC number: =2.7.7.13

Molecular weight: Translated: 56214; Mature: 56214

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHPLMRLLRSRHTVMKLPSNAIKNDTMLPKIIPAIMAGGRGTRLWPLSRATAAKQFLK
CCCCHHHHHHHCCCCEEECCCHHHCCCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
LIGEETLFQDTLKRVSDAKVYGAPLVITNEEFRFLVAEQARELGVTLSSIVLEPVPRNTA
HHCHHHHHHHHHHHHHCCEEECCCEEEECCCCEEHHHHHHHHHCCCHHHHHCCCCCCCHH
AAVAVAARIVADRFGEDALLLVLPSDHAITVDDTYKKCVRSACIAAAEGKLVTFGIQPTW
HHHHHHHHHHHHHCCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCC
PATGYGYIERGTYLGKDVHAVQCFVEKPSLEKAAALLETGNYYWNSGMFLFQAASIIAEL
CCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHH
EEHAPDVLSAVHAAVRGSTVDADFIRLAPESFSQAPSISIDYALMEKTANAAVVCSDFAW
HHHCHHHHHHHHHHHCCCCCCCHHHEECCHHHCCCCCCEEEHHHHHCCCCCEEEEECCCC
SDLGSWDAVWKNEEQNADGNVLKGNVTACNTKNSLVLSHTAHLAVQGMDGVAVIASEDAV
CCCCCCCHHCCCCCCCCCCCEEECCEEEECCCCCEEEEEEEEEEEECCCCEEEEECCCEE
FVGRLEEAHEIGNLVKRLAADENTARLTELHPTLIRPWGGYTTMLNGDRFQVRRLFVRPG
EEHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHCCCCCCEEEEECCCCEEEHHHHHCCC
KMLSLHKHFHRSEHWICVKGTAEVTIEDRVTILHENQSIYIPEGAIHRLGNPGKIMLELV
HHHHHHHHHCCCCCEEEEECCEEEEEECCEEEEECCCEEEECCHHHHHCCCCCCEEEEEE
EIQTGAYLGEDDIIRVADESRNEMPDSRRTGP
EECCCCEECCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSHPLMRLLRSRHTVMKLPSNAIKNDTMLPKIIPAIMAGGRGTRLWPLSRATAAKQFLK
CCCCHHHHHHHCCCCEEECCCHHHCCCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
LIGEETLFQDTLKRVSDAKVYGAPLVITNEEFRFLVAEQARELGVTLSSIVLEPVPRNTA
HHCHHHHHHHHHHHHHCCEEECCCEEEECCCCEEHHHHHHHHHCCCHHHHHCCCCCCCHH
AAVAVAARIVADRFGEDALLLVLPSDHAITVDDTYKKCVRSACIAAAEGKLVTFGIQPTW
HHHHHHHHHHHHHCCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCC
PATGYGYIERGTYLGKDVHAVQCFVEKPSLEKAAALLETGNYYWNSGMFLFQAASIIAEL
CCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHH
EEHAPDVLSAVHAAVRGSTVDADFIRLAPESFSQAPSISIDYALMEKTANAAVVCSDFAW
HHHCHHHHHHHHHHHCCCCCCCHHHEECCHHHCCCCCCEEEHHHHHCCCCCEEEEECCCC
SDLGSWDAVWKNEEQNADGNVLKGNVTACNTKNSLVLSHTAHLAVQGMDGVAVIASEDAV
CCCCCCCHHCCCCCCCCCCCEEECCEEEECCCCCEEEEEEEEEEEECCCCEEEEECCCEE
FVGRLEEAHEIGNLVKRLAADENTARLTELHPTLIRPWGGYTTMLNGDRFQVRRLFVRPG
EEHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHCCCCCCEEEEECCCCEEEHHHHHCCC
KMLSLHKHFHRSEHWICVKGTAEVTIEDRVTILHENQSIYIPEGAIHRLGNPGKIMLELV
HHHHHHHHHCCCCCEEEEECCEEEEEECCEEEEECCCEEEECCHHHHHCCCCCCEEEEEE
EIQTGAYLGEDDIIRVADESRNEMPDSRRTGP
EECCCCEECCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424