Click here to switch to the map view.

The map label for this gene is putP

Identifier: 16273262

GI number: 16273262

Start: 1428884

End: 1430398

Strand: Reverse

Name: putP

Synonym: HI1352

Alternate gene names: 16273262

Gene position: 1430398-1428884 (Counterclockwise)

Preceding gene: 16273274

Following gene: 16273261

Centisome position: 78.16

GC content: 39.67

Gene sequence:

>1515_bases
ATGTTTGGATTCGACCCAAGTCTTATTACTTTTACTATTTATATTTTCGGGATGTTGCTGATTGGCGTACTCGCCTATTA
TTACACGAATAATTTATCAGATTACATTCTCGGTGGACGTCGTTTAGGCAGTTTTGTTACTGCAATGTCTGCAGGTGCGT
CAGATATGTCTGGTTGGCTTTTAATGGGCTTACCTGGTGCGGTATATTTATCAGGCTTAGTTGAAGGCTGGATTGCTATT
GGTTTAACTATCGGGGCTTATTTTAACTGGCTTTTAGTGGCTGGTCGTTTGCGTGTTTATACAGAATTAAATAATAATGC
GCTCACTCTCCCAGAATATTTTCACAATCGTTTTGGTTCATCACACAAATTATTAAAACTTGTTTCTGCCACTATTATTT
TAGTGTTTTTAACTATTTATTGTGCTTCTGGTGTCGTGGCTGGCGCAAAATTATTCCAAAATATATTTTCTGTGGAATAT
TCCACCGCACTTTGGTACGGCGCAGCGGCAACCATTGCTTACACGTTCATCGGAGGTTTCCTTGCGGTAAGCTGGACAGA
TACCATTCAAGCCACATTAATGATTTTTGCATTAATTTTAACCCCTGTTTTTGTGTTATTGAGTTTCGCCGATACCGCTC
AATTTTCCGCAGTACTAGAACAAGCTGAGGCTGCCGTAAATAAAGATTTCACGGATTTATTTACTTCTACCACACCACTT
GGTTTATTAAGTCTTGCGGCTTGGGGATTAGGCTATTTCGGGCAACCGCATATTTTAGCACGCTTTATGGCTGCGGATTC
TGTCAAATCACTTATCAAAGCACGCCGTATTAGTATGGGTTGGATGGTGCTTTGCTTAGCAGGCGCAATTGGCATTGGCT
TATTCGCTATTCCGTATTTCTTTGCAAATCCAGCTATTGCAGGCACAGTTAATCGCGAACCAGAACAGGTTTTTATTGAA
TTAGCTAAACTTTTATTTAATCCTTGGATCGCAGGCATATTACTTTCCGCTATTTTAGCAGCAGTAATGAGTACATTAAG
TGCGCAATTGTTAATTTCCTCTAGCTCAATCACAGAAGATTTCTATAAAGGTTTTATTCGCCCTAACGCATCTGAAAAAG
AGCTCGTATGGCTTGGCAGAATTATGGTGTTAGTTATTGCCGCACTTGCTATCTGGATCGCACAAGATGAAAACAGCAAA
GTATTAAAACTTGTAGAATTTGCTTGGGCGGGGTTTGGTAGTGCATTTGGCCCTGTTGTACTTTTCTCTCTTTTCTGGAA
ACGAATGACATCATCGGGTGCAATGGCGGGTATGCTTGTAGGTGCAGTGACAGTATTTGCTTGGAAAGAAGTTGTTCCAG
CTGATACTGATTGGTTTAAAGTATATGAAATGATCCCAGGCTTTGCTTTCGCCAGCCTTGCAATTATTGTTATTTCATTA
CTTTCCAATAAACCAGAACAAGATATTCTTAATACCTTTGATAAAGCAGAAAAGGCTTATAAGGAAGCAAAATGA

Upstream 100 bases:

>100_bases
TCTTAAATTTCATCAAATATTTAGTCTAAATAGAATAATAGCCCGAAATAAGGTAAAATGCACCCTTTCTGAAATTTTTA
ACCCATTTTGGACTTATATA

Downstream 100 bases:

>100_bases
TCGACTTTCGCCCTTTTTATCAACAAATTGCTACTACAAATTTATCAGACTGGTTAGAGACCTTACCGTGCCAATTGAAA
GAATGGGAAACTCAAACTCA

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MFGFDPSLITFTIYIFGMLLIGVLAYYYTNNLSDYILGGRRLGSFVTAMSAGASDMSGWLLMGLPGAVYLSGLVEGWIAI
GLTIGAYFNWLLVAGRLRVYTELNNNALTLPEYFHNRFGSSHKLLKLVSATIILVFLTIYCASGVVAGAKLFQNIFSVEY
STALWYGAAATIAYTFIGGFLAVSWTDTIQATLMIFALILTPVFVLLSFADTAQFSAVLEQAEAAVNKDFTDLFTSTTPL
GLLSLAAWGLGYFGQPHILARFMAADSVKSLIKARRISMGWMVLCLAGAIGIGLFAIPYFFANPAIAGTVNREPEQVFIE
LAKLLFNPWIAGILLSAILAAVMSTLSAQLLISSSSITEDFYKGFIRPNASEKELVWLGRIMVLVIAALAIWIAQDENSK
VLKLVEFAWAGFGSAFGPVVLFSLFWKRMTSSGAMAGMLVGAVTVFAWKEVVPADTDWFKVYEMIPGFAFASLAIIVISL
LSNKPEQDILNTFDKAEKAYKEAK

Sequences:

>Translated_504_residues
MFGFDPSLITFTIYIFGMLLIGVLAYYYTNNLSDYILGGRRLGSFVTAMSAGASDMSGWLLMGLPGAVYLSGLVEGWIAI
GLTIGAYFNWLLVAGRLRVYTELNNNALTLPEYFHNRFGSSHKLLKLVSATIILVFLTIYCASGVVAGAKLFQNIFSVEY
STALWYGAAATIAYTFIGGFLAVSWTDTIQATLMIFALILTPVFVLLSFADTAQFSAVLEQAEAAVNKDFTDLFTSTTPL
GLLSLAAWGLGYFGQPHILARFMAADSVKSLIKARRISMGWMVLCLAGAIGIGLFAIPYFFANPAIAGTVNREPEQVFIE
LAKLLFNPWIAGILLSAILAAVMSTLSAQLLISSSSITEDFYKGFIRPNASEKELVWLGRIMVLVIAALAIWIAQDENSK
VLKLVEFAWAGFGSAFGPVVLFSLFWKRMTSSGAMAGMLVGAVTVFAWKEVVPADTDWFKVYEMIPGFAFASLAIIVISL
LSNKPEQDILNTFDKAEKAYKEAK
>Mature_504_residues
MFGFDPSLITFTIYIFGMLLIGVLAYYYTNNLSDYILGGRRLGSFVTAMSAGASDMSGWLLMGLPGAVYLSGLVEGWIAI
GLTIGAYFNWLLVAGRLRVYTELNNNALTLPEYFHNRFGSSHKLLKLVSATIILVFLTIYCASGVVAGAKLFQNIFSVEY
STALWYGAAATIAYTFIGGFLAVSWTDTIQATLMIFALILTPVFVLLSFADTAQFSAVLEQAEAAVNKDFTDLFTSTTPL
GLLSLAAWGLGYFGQPHILARFMAADSVKSLIKARRISMGWMVLCLAGAIGIGLFAIPYFFANPAIAGTVNREPEQVFIE
LAKLLFNPWIAGILLSAILAAVMSTLSAQLLISSSSITEDFYKGFIRPNASEKELVWLGRIMVLVIAALAIWIAQDENSK
VLKLVEFAWAGFGSAFGPVVLFSLFWKRMTSSGAMAGMLVGAVTVFAWKEVVPADTDWFKVYEMIPGFAFASLAIIVISL
LSNKPEQDILNTFDKAEKAYKEAK

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family

Homologues:

Organism=Homo sapiens, GI310128183, Length=501, Percent_Identity=45.1097804391218, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI14140236, Length=483, Percent_Identity=23.6024844720497, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI4507031, Length=550, Percent_Identity=24.7272727272727, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI110835708, Length=542, Percent_Identity=23.6162361623616, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI17941285, Length=408, Percent_Identity=25, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1787251, Length=498, Percent_Identity=62.0481927710843, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI1790503, Length=453, Percent_Identity=25.60706401766, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87082237, Length=500, Percent_Identity=25.8, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI115533094, Length=380, Percent_Identity=26.8421052631579, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221459584, Length=361, Percent_Identity=25.207756232687, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645928, Length=381, Percent_Identity=26.509186351706, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI221459588, Length=434, Percent_Identity=25.5760368663594, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651739, Length=435, Percent_Identity=23.448275862069, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI221459586, Length=387, Percent_Identity=21.7054263565891, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24651741, Length=432, Percent_Identity=23.6111111111111, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221459582, Length=370, Percent_Identity=24.8648648648649, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24650192, Length=401, Percent_Identity=23.6907730673317, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281362918, Length=437, Percent_Identity=25.1716247139588, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUTP_HAEIN (P45174)

Other databases:

- EMBL:   L42023
- PIR:   E64118
- RefSeq:   NP_439503.1
- GeneID:   950133
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1352
- NMPDR:   fig|71421.1.peg.1288
- TIGR:   HI_1352
- HOGENOM:   HBG499520
- OMA:   HILRIVC
- ProtClustDB:   CLSK870059
- BioCyc:   HINF71421:HI_1352-MONOMER
- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900
- PANTHER:   PTHR11819
- TIGRFAMs:   TIGR02121
- TIGRFAMs:   TIGR00813

Pfam domain/function: PF00474 SSF

EC number: NA

Molecular weight: Translated: 54899; Mature: 54899

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00456 NA_SOLUT_SYMP_1; PS00457 NA_SOLUT_SYMP_2; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe211850)-; HASH(0xe47d824)-; HASH(0xe7a1d78)-; HASH(0xe15e538)-; HASH(0xc99f52c)-; HASH(0xdc9e590)-; HASH(0xc847c5c)-; HASH(0xe2b1d34)-; HASH(0xe542798)-; HASH(0xe72d600)-; HASH(0xb667df8)-; HASH(0x8b00714)-; HASH(0xdbfb430)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGFDPSLITFTIYIFGMLLIGVLAYYYTNNLSDYILGGRRLGSFVTAMSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCCEE
LMGLPGAVYLSGLVEGWIAIGLTIGAYFNWLLVAGRLRVYTELNNNALTLPEYFHNRFGS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEECHHHHHHHCCC
SHKLLKLVSATIILVFLTIYCASGVVAGAKLFQNIFSVEYSTALWYGAAATIAYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTIQATLMIFALILTPVFVLLSFADTAQFSAVLEQAEAAVNKDFTDLFTSTTPL
HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHH
GLLSLAAWGLGYFGQPHILARFMAADSVKSLIKARRISMGWMVLCLAGAIGIGLFAIPYF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FANPAIAGTVNREPEQVFIELAKLLFNPWIAGILLSAILAAVMSTLSAQLLISSSSITED
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FYKGFIRPNASEKELVWLGRIMVLVIAALAIWIAQDENSKVLKLVEFAWAGFGSAFGPVV
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHH
LFSLFWKRMTSSGAMAGMLVGAVTVFAWKEVVPADTDWFKVYEMIPGFAFASLAIIVISL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
LSNKPEQDILNTFDKAEKAYKEAK
HCCCCHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MFGFDPSLITFTIYIFGMLLIGVLAYYYTNNLSDYILGGRRLGSFVTAMSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCCEE
LMGLPGAVYLSGLVEGWIAIGLTIGAYFNWLLVAGRLRVYTELNNNALTLPEYFHNRFGS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEECHHHHHHHCCC
SHKLLKLVSATIILVFLTIYCASGVVAGAKLFQNIFSVEYSTALWYGAAATIAYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTIQATLMIFALILTPVFVLLSFADTAQFSAVLEQAEAAVNKDFTDLFTSTTPL
HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHH
GLLSLAAWGLGYFGQPHILARFMAADSVKSLIKARRISMGWMVLCLAGAIGIGLFAIPYF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FANPAIAGTVNREPEQVFIELAKLLFNPWIAGILLSAILAAVMSTLSAQLLISSSSITED
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FYKGFIRPNASEKELVWLGRIMVLVIAALAIWIAQDENSKVLKLVEFAWAGFGSAFGPVV
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHH
LFSLFWKRMTSSGAMAGMLVGAVTVFAWKEVVPADTDWFKVYEMIPGFAFASLAIIVISL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
LSNKPEQDILNTFDKAEKAYKEAK
HCCCCHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800