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The map label for this gene is bcr

Identifier: 16273161

GI number: 16273161

Start: 1315231

End: 1316427

Strand: Reverse

Name: bcr

Synonym: HI1242

Alternate gene names: 16273161

Gene position: 1316427-1315231 (Counterclockwise)

Preceding gene: 16273162

Following gene: 16273159

Centisome position: 71.93

GC content: 41.44

Gene sequence:

>1197_bases
ATGAATCAACAAAAATCGACTTTCATCTTTATTCTTACCCTCGGTATTCTCTCTATGCTACCGCCCTTTGGCGTGGATAT
GTATTTGCCGTCTTTTTTAGAAATTGCAAAGGATCTCGACGTAAGTCCAGAACAAGTACAACACACGCTCACCTCTTTTG
CTTATGGCATGGCGTTTGGTCAGCTTTTTTGGGGGCCTTTTGGTGATAGTTTCGGACGAAAACCGATCATTTTACTCGGT
GTTATTGTGGGCGCATTAACAGCTTTAGTGCTTACCGAAATAAATTCAGTTGGAAATTTTACCGCACTTCGTTTTGTGCA
AGGTTTCTTTGGTGCCGCACCTGTTGTACTTTCTGGCGCATTATTGCGTGATTTATTTAGTAAAGATCAGCTATCCAAAG
TGATGTCCACCATCACGTTAGTATTTATGCTTGCACCTTTAGTTGCACCAATCATTGGTGGTTACATCGTTAAATTTTTC
CATTGGCATGCGATTTTTTACGTTATTTCGCTCGTGGGATTATTAGCCGCTGCATTGGTCTTTTTCATCATTCCAGAAAC
CCATAAAAAAGAAAATCGCATTCCGCTGCGCTTAAATATTATCGCTCGCAATTTCCTTCTGCTTTGGAAGCAAAAAGAAG
TGCTTGGCTATATGTTTGCCGCCTCTTTTAGCTTTGGTGGATTGTTTGCTTTTGTGACTGCAGGATCAATTGTCTATATC
GGCATTTATGGCGTTCCCGTCGATCAGTTTGGTTATTTCTTTATGATGAATATCGTTACGATGATCTTTGCCTCTTTTTT
AAATAGCCGATTTGTTACTAAAGTGGGTGCAGAAACAATGTTACGGATAGCCCTCGCAATTCAATTCCTTTCTGGAATGT
GGCTAATTTTGACCGCACTTTTAGATCTCGGTTTTTGGCCAATGGCAATTGGCGTCGCCTTTTTCGTCGGTCCAAACCCA
GTGATTTCATCAAATGCAATGGCATCAGCTTTAGAAAGATGTCCTCAAATGGCAGGAACAGCAAATTCTTTGATTGGTAG
CGTACGTTTTGCAGTGGGTGCAATTATGGGAAGTTTAGTCGCGTCAATGAAAATGGATACTGCCGCACCAATGCTCTTTA
CTATGGGGGCATGTGTCGTGATTTCCGTGTTGGCGTATTACTTTCTAACCAGCCGAAATCTTAAAAGCCGTGGATAA

Upstream 100 bases:

>100_bases
CATCGCTGGAAAATTGGTGATGTAGTGTTGGATGAATCCTTAGAAGAAGGCGAATACCGCCCATTAACTCAAAGTGAAAT
TGAAAAGTTAGTAAAATAAT

Downstream 100 bases:

>100_bases
ATTCCCCACGGCGCAATGATTGTTTTCTTTATTAAACAAAAACCATCCATAACGGCAAGCAAACAGCAATAAATAATAGG
ATAAATAAACTATTGGTAGA

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNQQKSTFIFILTLGILSMLPPFGVDMYLPSFLEIAKDLDVSPEQVQHTLTSFAYGMAFGQLFWGPFGDSFGRKPIILLG
VIVGALTALVLTEINSVGNFTALRFVQGFFGAAPVVLSGALLRDLFSKDQLSKVMSTITLVFMLAPLVAPIIGGYIVKFF
HWHAIFYVISLVGLLAAALVFFIIPETHKKENRIPLRLNIIARNFLLLWKQKEVLGYMFAASFSFGGLFAFVTAGSIVYI
GIYGVPVDQFGYFFMMNIVTMIFASFLNSRFVTKVGAETMLRIALAIQFLSGMWLILTALLDLGFWPMAIGVAFFVGPNP
VISSNAMASALERCPQMAGTANSLIGSVRFAVGAIMGSLVASMKMDTAAPMLFTMGACVVISVLAYYFLTSRNLKSRG

Sequences:

>Translated_398_residues
MNQQKSTFIFILTLGILSMLPPFGVDMYLPSFLEIAKDLDVSPEQVQHTLTSFAYGMAFGQLFWGPFGDSFGRKPIILLG
VIVGALTALVLTEINSVGNFTALRFVQGFFGAAPVVLSGALLRDLFSKDQLSKVMSTITLVFMLAPLVAPIIGGYIVKFF
HWHAIFYVISLVGLLAAALVFFIIPETHKKENRIPLRLNIIARNFLLLWKQKEVLGYMFAASFSFGGLFAFVTAGSIVYI
GIYGVPVDQFGYFFMMNIVTMIFASFLNSRFVTKVGAETMLRIALAIQFLSGMWLILTALLDLGFWPMAIGVAFFVGPNP
VISSNAMASALERCPQMAGTANSLIGSVRFAVGAIMGSLVASMKMDTAAPMLFTMGACVVISVLAYYFLTSRNLKSRG
>Mature_398_residues
MNQQKSTFIFILTLGILSMLPPFGVDMYLPSFLEIAKDLDVSPEQVQHTLTSFAYGMAFGQLFWGPFGDSFGRKPIILLG
VIVGALTALVLTEINSVGNFTALRFVQGFFGAAPVVLSGALLRDLFSKDQLSKVMSTITLVFMLAPLVAPIIGGYIVKFF
HWHAIFYVISLVGLLAAALVFFIIPETHKKENRIPLRLNIIARNFLLLWKQKEVLGYMFAASFSFGGLFAFVTAGSIVYI
GIYGVPVDQFGYFFMMNIVTMIFASFLNSRFVTKVGAETMLRIALAIQFLSGMWLILTALLDLGFWPMAIGVAFFVGPNP
VISSNAMASALERCPQMAGTANSLIGSVRFAVGAIMGSLVASMKMDTAAPMLFTMGACVVISVLAYYFLTSRNLKSRG

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family

Homologues:

Organism=Escherichia coli, GI1788509, Length=383, Percent_Identity=42.8198433420365, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI48994889, Length=183, Percent_Identity=32.2404371584699, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1790146, Length=172, Percent_Identity=31.3953488372093, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082312, Length=349, Percent_Identity=25.214899713467, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790794, Length=309, Percent_Identity=23.3009708737864, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787065, Length=386, Percent_Identity=24.6113989637306, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321838, Length=162, Percent_Identity=29.0123456790123, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BCR_HAEIN (P45123)

Other databases:

- EMBL:   L42023
- PIR:   E64112
- RefSeq:   NP_439398.1
- ProteinModelPortal:   P45123
- SMR:   P45123
- GeneID:   949578
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1242
- NMPDR:   fig|71421.1.peg.1188
- TIGR:   HI_1242
- HOGENOM:   HBG300735
- OMA:   YFLTYRS
- ProtClustDB:   PRK11102
- BioCyc:   HINF71421:HI_1242-MONOMER
- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00710

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 43460; Mature: 43460

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd0478a8)-; HASH(0xaf8d834)-; HASH(0xe0f9490)-; HASH(0xdf53040)-; HASH(0xc7bd050)-; HASH(0xdf87000)-; HASH(0xd621d30)-; HASH(0xd5efeac)-; HASH(0xc5651a8)-; HASH(0xe0d733c)-; HASH(0xb1d79fc)-; HASH(0xde3474c)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800