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The map label for this gene is ptsN

Identifier: 16273073

GI number: 16273073

Start: 1216446

End: 1216940

Strand: Reverse

Name: ptsN

Synonym: HI1147

Alternate gene names: 16273073

Gene position: 1216940-1216446 (Counterclockwise)

Preceding gene: 16273074

Following gene: 30995425

Centisome position: 66.49

GC content: 35.35

Gene sequence:

>495_bases
ATGAAAATAACTGAACTATTAAGCCCTGAAAATATTCGTCAGGGCGTTAGTTTTTCTAGCAAAAAACGATTATTTGAGTC
TATTGCGCATTTTGTAGAAGAACAAATCCTTGCAGAAAAAGGCGAACAAGCCTGTTTTGAATGCCTTTTTGAACGTGAAA
AACTGGGGAATTCTGGTTTAGGAAATGGGATTGCAATGCCAAAAGCGAAAATCCCCGTAACAGTATCAGATAAAGCAATC
GCAGTGTTTATGCAACTCGATAATCCGATTGATTACGATGCTTTTGATGGTAAACCAGTGGATTTAATCTTTGCCTTGCT
AATTCCAGAAAATCAATGTGAAACCTACATTCCAGTTTTAGCATCATTAATAGAAAAACTGACTGATAAAAATGTGTTGA
AACAATTACGATCAGCCAAAAGTGCGGATGAAATTTGGCAAGTTTTTGAAATTACCGATCAATCAGAAACGACATTTGAA
GAAGTGAAAGAATAA

Upstream 100 bases:

>100_bases
TTGGCGCAGGTAAAATTATCGCAACAGGCACACCAGAACAAGTGATGAACGATGAACAGGTTAAACGCGTTTATCTTGGC
GAACAATTCAAATTATAAAA

Downstream 100 bases:

>100_bases
TTACAGTGGGGAAACTTATGGAAATTATCATTATTAGTGGACGTTCTGGCGCAGGAAAATCTGTAGCCTTACGAGCATTA
GAAGATGCCGGATATTATTG

Product: nitrogen regulatory IIA protein

Products: protein histidine; sugar phosphate

Alternate protein names: Putative phosphotransferase enzyme IIA component; Putative PTS system EIIA component

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEKGEQACFECLFEREKLGNSGLGNGIAMPKAKIPVTVSDKAI
AVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVLASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFE
EVKE

Sequences:

>Translated_164_residues
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEKGEQACFECLFEREKLGNSGLGNGIAMPKAKIPVTVSDKAI
AVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVLASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFE
EVKE
>Mature_164_residues
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEKGEQACFECLFEREKLGNSGLGNGIAMPKAKIPVTVSDKAI
AVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVLASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFE
EVKE

Specific function: Not known; lacks the phosphorylation site found in other ptsN proteins

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain

Homologues:

Organism=Escherichia coli, GI1789597, Length=157, Percent_Identity=38.8535031847134, Blast_Score=121, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTSN_HAEIN (P45072)

Other databases:

- EMBL:   L42023
- PIR:   H64167
- RefSeq:   NP_439305.1
- ProteinModelPortal:   P45072
- SMR:   P45072
- GeneID:   950111
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1147
- NMPDR:   fig|71421.1.peg.1100
- TIGR:   HI_1147
- HOGENOM:   HBG697201
- OMA:   CTSKKRA
- ProtClustDB:   CLSK808489
- BioCyc:   HINF71421:HI_1147-MONOMER
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320
- Gene3D:   G3DSA:3.40.930.10
- TIGRFAMs:   TIGR01419

Pfam domain/function: PF00359 PTS_EIIA_2; SSF55804 PTrfase/Anion_transptr

EC number: 2.7.1.69

Molecular weight: Translated: 18444; Mature: 18444

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEKGEQACFECLFEREKLGNSGL
CCCHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
GNGIAMPKAKIPVTVSDKAIAVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVL
CCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
ASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFEEVKE
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure
MKITELLSPENIRQGVSFSSKKRLFESIAHFVEEQILAEKGEQACFECLFEREKLGNSGL
CCCHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
GNGIAMPKAKIPVTVSDKAIAVFMQLDNPIDYDAFDGKPVDLIFALLIPENQCETYIPVL
CCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
ASLIEKLTDKNVLKQLRSAKSADEIWQVFEITDQSETTFEEVKE
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800