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The map label for this gene is amiB

Identifier: 16272040

GI number: 16272040

Start: 68915

End: 70213

Strand: Direct

Name: amiB

Synonym: HI0066

Alternate gene names: 16272040

Gene position: 68915-70213 (Clockwise)

Preceding gene: 16272039

Following gene: 16272041

Centisome position: 3.77

GC content: 32.56

Gene sequence:

>1299_bases
ATGAAAACTAAAATTTTATTTTTCCTTTTTTTCTCAACGTTTAGTTTCTCTATTTTTGCAGCACCTATTACGATTGCGAT
TGACCCAGGTCATGGGGGAAAAGATCCTGGTGCAATCAGCCGAAACTTAGGCATTTATGAAAAAAATGTTACCCTTTCAA
TTGCAAAAGAATTAAAAGCCTTATTAGATAAAGATCCACATTTTCGCGGTGTATTAACGCGTAAAAGTGATTATTATATT
TCCGTGCCAGAACGTTCAGAAATCGCACGTAAATTTAAAGCGAATTATCTTATTTCCATTCATGCCGATTCATCTAAATC
ACCTGATCGTCGCGGGGCTTCTGTTTGGGTATTGTCAAACCGTCGAGCCAATGATGAAATGGGTCAATGGTTAGAAGACG
ATGAAAAACGCTCGGAATTACTTGGTGGAGCGGGAAAGGTGCTTTCACACAATAATGACAAATATCTTGACCAGACTGTA
CTTGATCTGCAATTCGGACATAGCCAACGTACAGGCTATGTGTTAGGCGAACATATTTTGCACCATTTTGCTAAAGTAAC
AACATTAAGTCGCAGTACGCCACAACATGCAAGTCTGGGTGTTCTACGTTCGCCAGATATTCCTTCTGTGTTGGTAGAAA
CTGGATTTCTTTCCAATTCAGAAGAAGAAAAAAAACTTAATTCCCAAACTTATCGTCGCCGCATTGCTTATATGATTTAT
GAAGGCTTAGTTGCATTCCATAGCGGTAAAACCAACACTTTAGTTAAAGACAATTTAGTGCAAAACATCAAACAAAACGA
TATAAAAAAATCAGGGAAAAATAACCGCACTTCAGAACAAAATATAAATGAAGATAATATTAAAGACAGTGGCATTCGCC
ATATTGTAAAAAAAGGAGAAAGTTTAGGTAGCTTATCAAATAAATATCACGTAAAAGTCTCTGATATTATTAAATTAAAT
CAATTAAAGAGAAAAACACTTTGGCTAAATGAAAGTATTAAAATCCCTGATAATGTCGAGATAAAAAATAAATCATTAAC
AATTAAAGAAAATGATTTTCATAAAAAACAAAATAGCTTAGTAAATAATACCAATAAAGATTTAAAAAAAGAGAAAAATA
CACAAACTAATAATCAAAAAAATATAATTCCGCTCTATCATAAAGTAACAAAAAACCAAACACTTTATGCTATTTCGAGG
GAATATAACATTCCAGTAAATATACTATTGAGCCTAAATCCACATTTAAAAAATGGCAAAGTTATTACAGGACAAAAAAT
AAAATTAAGAGAAAAATAA

Upstream 100 bases:

>100_bases
GTGAATATTGATTATTACGATGATGCACGAAATATTGAATTAATCGCACAAACAAATTTGGGTAAAAATATTATAAGCGC
ATTTTCTAATTAACTTATTT

Downstream 100 bases:

>100_bases
ATGCCTATTAAAATTCTTTCCCCACAACTTGCTAACCAAATTGCTGCTGGAGAAGTGGTAGAACGCCCTGCATCAGTGGT
AAAAGAATTAGTGGAAAATA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAISRNLGIYEKNVTLSIAKELKALLDKDPHFRGVLTRKSDYYI
SVPERSEIARKFKANYLISIHADSSKSPDRRGASVWVLSNRRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTV
LDLQFGHSQRTGYVLGEHILHHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLNSQTYRRRIAYMIY
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGESLGSLSNKYHVKVSDIIKLN
QLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSLVNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISR
EYNIPVNILLSLNPHLKNGKVITGQKIKLREK

Sequences:

>Translated_432_residues
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAISRNLGIYEKNVTLSIAKELKALLDKDPHFRGVLTRKSDYYI
SVPERSEIARKFKANYLISIHADSSKSPDRRGASVWVLSNRRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTV
LDLQFGHSQRTGYVLGEHILHHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLNSQTYRRRIAYMIY
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGESLGSLSNKYHVKVSDIIKLN
QLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSLVNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISR
EYNIPVNILLSLNPHLKNGKVITGQKIKLREK
>Mature_432_residues
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAISRNLGIYEKNVTLSIAKELKALLDKDPHFRGVLTRKSDYYI
SVPERSEIARKFKANYLISIHADSSKSPDRRGASVWVLSNRRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTV
LDLQFGHSQRTGYVLGEHILHHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLNSQTYRRRIAYMIY
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGESLGSLSNKYHVKVSDIIKLN
QLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSLVNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISR
EYNIPVNILLSLNPHLKNGKVITGQKIKLREK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats

Homologues:

Organism=Escherichia coli, GI1790611, Length=222, Percent_Identity=59.4594594594595, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1788776, Length=222, Percent_Identity=39.6396396396396, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI87082163, Length=231, Percent_Identity=36.3636363636364, Blast_Score=158, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMIB_HAEIN (P44493)

Other databases:

- EMBL:   L42023
- PIR:   D64046
- RefSeq:   NP_438239.1
- ProteinModelPortal:   P44493
- SMR:   P44493
- GeneID:   950965
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0066
- NMPDR:   fig|71421.1.peg.65
- TIGR:   HI_0066
- HOGENOM:   HBG656726
- OMA:   QKIKLRE
- ProtClustDB:   CLSK2518355
- BioCyc:   HINF71421:HI_0066-MONOMER
- BRENDA:   3.5.1.28
- InterPro:   IPR002508
- InterPro:   IPR018392
- InterPro:   IPR002482
- Gene3D:   G3DSA:3.40.630.40
- SMART:   SM00646
- SMART:   SM00257

Pfam domain/function: PF01520 Amidase_3; PF01476 LysM

EC number: =3.5.1.28

Molecular weight: Translated: 49110; Mature: 49110

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAISRNLGIYEKNVTLSIAKELKA
CCCEEEEEEEHHHHCCEEEEEEEEEEEECCCCCCCCCCHHCCCCEEECCCHHHHHHHHHH
LLDKDPHFRGVLTRKSDYYISVPERSEIARKFKANYLISIHADSSKSPDRRGASVWVLSN
HHCCCCCCEEEEEECCCEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEC
RRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTVLDLQFGHSQRTGYVLGEHIL
CCCCHHHHHHHCCHHHHHHHHCCCCHHHCCCCCHHHHHHHHEEECCCCCCCCHHHHHHHH
HHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLNSQTYRRRIAYMIY
HHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGE
HHHHHEECCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
SLGSLSNKYHVKVSDIIKLNQLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSL
HHHCCCCEEEEEHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCEEEEECCHHHHHHHHH
VNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISREYNIPVNILLSLNPHLKNGK
HCCCCHHHHHHCCCCCCCCCCEEEHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCE
VITGQKIKLREK
EEECCEEEECCC
>Mature Secondary Structure
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAISRNLGIYEKNVTLSIAKELKA
CCCEEEEEEEHHHHCCEEEEEEEEEEEECCCCCCCCCCHHCCCCEEECCCHHHHHHHHHH
LLDKDPHFRGVLTRKSDYYISVPERSEIARKFKANYLISIHADSSKSPDRRGASVWVLSN
HHCCCCCCEEEEEECCCEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEC
RRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTVLDLQFGHSQRTGYVLGEHIL
CCCCHHHHHHHCCHHHHHHHHCCCCHHHCCCCCHHHHHHHHEEECCCCCCCCHHHHHHHH
HHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLNSQTYRRRIAYMIY
HHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGE
HHHHHEECCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
SLGSLSNKYHVKVSDIIKLNQLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSL
HHHCCCCEEEEEHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCEEEEECCHHHHHHHHH
VNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISREYNIPVNILLSLNPHLKNGK
HCCCCHHHHHHCCCCCCCCCCEEEHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCE
VITGQKIKLREK
EEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800