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The map label for this gene is rffA [H]

Identifier: 162421595

GI number: 162421595

Start: 543114

End: 544280

Strand: Direct

Name: rffA [H]

Synonym: YpAngola_A0519

Alternate gene names: 162421595

Gene position: 543114-544280 (Clockwise)

Preceding gene: 162421400

Following gene: 162420354

Centisome position: 12.06

GC content: 48.93

Gene sequence:

>1167_bases
TTGAGAGTACCGCGTATTGGTTATGCAGGGGATGAGATGATTCCATTTAACACTCCACCGATAGTGGGCACTGAACTGGG
TTATATGCAGGCTGCGATGAGCAGTGGCAAGTTATGCGGTGATGGTGGTTTTACCCGTCGCTGCCAGCAGTGGATGGAGA
AACGTTTTAACTGCCCAAAAGTGTTACTGACGCCATCTTGTACCGCCTCGTTGGAAATGGCCGCGTTATTGCTGGATATC
AAGCCCGGTGATGAAGTGATCATGCCAAGTTTTACCTTTGTTTCTACCGCCAATGCCTTTGTGCTGCGCGGGGCCAAAAT
GGTGTTTGTCGATATCCGTCCAGACACCATGAATATCGATGAAACCAAGATTGAAGCGGCGATCACCGATAAAACCCGGG
TTATTGTGCCAGTCCACTACGCAGGGGTTGCCTGTGAGATGGATACCATCATGGCACTGGCGAAAAAGCATAATTTGTTT
GTGGTAGAAGATGCGGCTCAAGGCGTGATGTCAACCTACAAAGGTAAAGCTCTGGGTACCATTGGCCATATTGGCTGCTT
CAGCTTCCATGAAACCAAAAATTACACTGCGGGCGGTGAAGGTGGGGCGACGTTGATCAATGATCCTTCATTGATTGATC
GTGCAGAAATTATCCGTGAAAAGGGCACCAACCGTAGCCAATTCTTCCGTGGTCAGGTGGATAAATACACCTGGCGTGAC
ATCGGCTCCAGCTACTTGATGTCTGATTTACAGGCCGCTTACCTGTGGGGCCAGTTAGAGGCAGCAGAGCAGATCAACGA
ACGCCGTCTGGCGCTATGGCATGGTTATTATAATGCTTTCAAACCGTTGGCCGATGCTGGGCGCATTGATTTACCGGTGA
TCCCAGGCAACGTGGTGCAAAATGCCCACATGTTTTATATTAAACTGCGTGATATTGAGGAGCGCTCTGCATTTATCAGC
TATTTAAAAGAAGCGGATATTATGGCGGTATTCCACTACATCCCATTACATGCGTGTCCAGCAGGTGAGGCGTTTGGCCG
TATGGCTGGCGAAGACCGCTTTACCTCCAAGGAGAGTGAGCGTCTGGTGCGCTTACCTATTTTCTACAACCTGACTGATG
TTAATCAGAGCACGGTTATTAATACTGTGCTGAGTTTCTTCGTCTGA

Upstream 100 bases:

>100_bases
AAGCAGTGGTGCCAGCATCATGGGTTACACCGGTTACGGGTAGCCACACAAATGAGCAATATTGCTGCTTTACGTCTCTA
TATTCGTAGTGGTGCCTCCA

Downstream 100 bases:

>100_bases
TATGTCTCTGGCAAAAGCATCGATTTGGACTGCCTGCTCTACGCTAATCAAGATTGGCGTAGGGCTATTGGTGGTTAAAC
TGCTGGCGGTCACCTTTGGC

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MRVPRIGYAGDEMIPFNTPPIVGTELGYMQAAMSSGKLCGDGGFTRRCQQWMEKRFNCPKVLLTPSCTASLEMAALLLDI
KPGDEVIMPSFTFVSTANAFVLRGAKMVFVDIRPDTMNIDETKIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLF
VVEDAAQGVMSTYKGKALGTIGHIGCFSFHETKNYTAGGEGGATLINDPSLIDRAEIIREKGTNRSQFFRGQVDKYTWRD
IGSSYLMSDLQAAYLWGQLEAAEQINERRLALWHGYYNAFKPLADAGRIDLPVIPGNVVQNAHMFYIKLRDIEERSAFIS
YLKEADIMAVFHYIPLHACPAGEAFGRMAGEDRFTSKESERLVRLPIFYNLTDVNQSTVINTVLSFFV

Sequences:

>Translated_388_residues
MRVPRIGYAGDEMIPFNTPPIVGTELGYMQAAMSSGKLCGDGGFTRRCQQWMEKRFNCPKVLLTPSCTASLEMAALLLDI
KPGDEVIMPSFTFVSTANAFVLRGAKMVFVDIRPDTMNIDETKIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLF
VVEDAAQGVMSTYKGKALGTIGHIGCFSFHETKNYTAGGEGGATLINDPSLIDRAEIIREKGTNRSQFFRGQVDKYTWRD
IGSSYLMSDLQAAYLWGQLEAAEQINERRLALWHGYYNAFKPLADAGRIDLPVIPGNVVQNAHMFYIKLRDIEERSAFIS
YLKEADIMAVFHYIPLHACPAGEAFGRMAGEDRFTSKESERLVRLPIFYNLTDVNQSTVINTVLSFFV
>Mature_388_residues
MRVPRIGYAGDEMIPFNTPPIVGTELGYMQAAMSSGKLCGDGGFTRRCQQWMEKRFNCPKVLLTPSCTASLEMAALLLDI
KPGDEVIMPSFTFVSTANAFVLRGAKMVFVDIRPDTMNIDETKIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLF
VVEDAAQGVMSTYKGKALGTIGHIGCFSFHETKNYTAGGEGGATLINDPSLIDRAEIIREKGTNRSQFFRGQVDKYTWRD
IGSSYLMSDLQAAYLWGQLEAAEQINERRLALWHGYYNAFKPLADAGRIDLPVIPGNVVQNAHMFYIKLRDIEERSAFIS
YLKEADIMAVFHYIPLHACPAGEAFGRMAGEDRFTSKESERLVRLPIFYNLTDVNQSTVINTVLSFFV

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=375, Percent_Identity=84.8, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI145693159, Length=385, Percent_Identity=30.9090909090909, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 43208; Mature: 43208

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPRIGYAGDEMIPFNTPPIVGTELGYMQAAMSSGKLCGDGGFTRRCQQWMEKRFNCPK
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCE
VLLTPSCTASLEMAALLLDIKPGDEVIMPSFTFVSTANAFVLRGAKMVFVDIRPDTMNID
EEECCCCCHHHEEEEEEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEECCCCCCCC
ETKIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMSTYKGKALGT
HHHEEEEECCCEEEEEEEEECCEEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCC
IGHIGCFSFHETKNYTAGGEGGATLINDPSLIDRAEIIREKGTNRSQFFRGQVDKYTWRD
HHHHEEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCCCHHHH
IGSSYLMSDLQAAYLWGQLEAAEQINERRLALWHGYYNAFKPLADAGRIDLPVIPGNVVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCEEEEECCCHHHC
NAHMFYIKLRDIEERSAFISYLKEADIMAVFHYIPLHACPAGEAFGRMAGEDRFTSKESE
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCC
RLVRLPIFYNLTDVNQSTVINTVLSFFV
CEEEEEEEEEEECCCHHHHHHHHHHHHC
>Mature Secondary Structure
MRVPRIGYAGDEMIPFNTPPIVGTELGYMQAAMSSGKLCGDGGFTRRCQQWMEKRFNCPK
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCE
VLLTPSCTASLEMAALLLDIKPGDEVIMPSFTFVSTANAFVLRGAKMVFVDIRPDTMNID
EEECCCCCHHHEEEEEEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEECCCCCCCC
ETKIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMSTYKGKALGT
HHHEEEEECCCEEEEEEEEECCEEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCC
IGHIGCFSFHETKNYTAGGEGGATLINDPSLIDRAEIIREKGTNRSQFFRGQVDKYTWRD
HHHHEEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCCCHHHH
IGSSYLMSDLQAAYLWGQLEAAEQINERRLALWHGYYNAFKPLADAGRIDLPVIPGNVVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCEEEEECCCHHHC
NAHMFYIKLRDIEERSAFISYLKEADIMAVFHYIPLHACPAGEAFGRMAGEDRFTSKESE
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCC
RLVRLPIFYNLTDVNQSTVINTVLSFFV
CEEEEEEEEEEECCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]