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The map label for this gene is phoB [H]

Identifier: 162149578

GI number: 162149578

Start: 3900973

End: 3901692

Strand: Direct

Name: phoB [H]

Synonym: GDI_3817

Alternate gene names: 162149578

Gene position: 3900973-3901692 (Clockwise)

Preceding gene: 162149577

Following gene: 162149579

Centisome position: 98.9

GC content: 68.19

Gene sequence:

>720_bases
ATGGCGAACGGGAACGAAGGTCAGACGAACGGCCGCAAATCGTACATCCTGGTGGTCGAGGATGACGAGGCGCTGCTGAT
GATGCTGCGCTACAACCTGGAAAAACTGGGATACGAGGTCGGCGTGGCCGAGGACGGGGAGCAGGCCCTGGCGCGTGTCG
CCGTCCGGCGTCCCGACCTGATGGTGCTGGACTGGATGCTGCCGGGCCTGTCGGGGCTGGATGTCTGCCGCCGGCTGCGT
GACCAGGAAGCGACCCGCACCCTGCCGATCATCATGCTGACCGCCCGCGCGGGCGAGGCCGACCATATTCGCGGCCTGGA
TATCGGCGCCGACGATTACGTCACCAAGCCCTGCAGCATCGAGGCGCTGCAGGCCCGCATCCGCGCACTGATGCGCCGGG
CGCCGCCGGCCGAGCAGATGCTGAGCTTCGCCGACGTCACGCTGGACACCGTCGCTCACCGGGCCGAGCGCAACGGAAGG
CAACTGACCCTGGGGCCGACCGAATACCGCATCCTGGAATTCTTCCTGCGCCATCCGCGCCAGGTCTTCTCGCGCGAGGA
CCTGTTGCAGCGGGTGTGGGAACGCGGGGTGCATGTGGAACCGCGGACGGTGGACGTGCATATCCGCCGGCTGCGGCTGA
TCCTGAACGGGCCGGGCGAGGCCGATATCGTCCGCACCGTGCGGGCGGCGGGCTACGCCCTGGACGATCCGCGTCCTTGA

Upstream 100 bases:

>100_bases
CGCCGAGCGCGTCTATTACGCCGCGACCGGCGAGCAACTGCCTGTCGCGCGGCCGCGCGGCGGCCCGGTGGTGTGACGGA
GTAGCGGAATGTCCGGAACG

Downstream 100 bases:

>100_bases
CGGCGCGGATCGGTCCGGGCAGCGGCGGGACCTAGGCATGGCCGGATTGTGGCCCGAGGATGTCGTGGCGGGCATGGCCC
TGCTGGCGGCGGGCGGCATG

Product: phosphate regulon transcriptional regulatory protein phoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MANGNEGQTNGRKSYILVVEDDEALLMMLRYNLEKLGYEVGVAEDGEQALARVAVRRPDLMVLDWMLPGLSGLDVCRRLR
DQEATRTLPIIMLTARAGEADHIRGLDIGADDYVTKPCSIEALQARIRALMRRAPPAEQMLSFADVTLDTVAHRAERNGR
QLTLGPTEYRILEFFLRHPRQVFSREDLLQRVWERGVHVEPRTVDVHIRRLRLILNGPGEADIVRTVRAAGYALDDPRP

Sequences:

>Translated_239_residues
MANGNEGQTNGRKSYILVVEDDEALLMMLRYNLEKLGYEVGVAEDGEQALARVAVRRPDLMVLDWMLPGLSGLDVCRRLR
DQEATRTLPIIMLTARAGEADHIRGLDIGADDYVTKPCSIEALQARIRALMRRAPPAEQMLSFADVTLDTVAHRAERNGR
QLTLGPTEYRILEFFLRHPRQVFSREDLLQRVWERGVHVEPRTVDVHIRRLRLILNGPGEADIVRTVRAAGYALDDPRP
>Mature_238_residues
ANGNEGQTNGRKSYILVVEDDEALLMMLRYNLEKLGYEVGVAEDGEQALARVAVRRPDLMVLDWMLPGLSGLDVCRRLRD
QEATRTLPIIMLTARAGEADHIRGLDIGADDYVTKPCSIEALQARIRALMRRAPPAEQMLSFADVTLDTVAHRAERNGRQ
LTLGPTEYRILEFFLRHPRQVFSREDLLQRVWERGVHVEPRTVDVHIRRLRLILNGPGEADIVRTVRAAGYALDDPRP

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=47.0319634703196, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=34.8214285714286, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1788394, Length=221, Percent_Identity=37.10407239819, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.9912663755458, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=34.8017621145374, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786911, Length=183, Percent_Identity=38.7978142076503, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=31.9634703196347, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=34.3891402714932, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=34.2342342342342, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=37.2294372294372, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=36.8181818181818, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=36.2445414847162, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=32.7510917030568, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=30.9012875536481, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI48994928, Length=116, Percent_Identity=38.7931034482759, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=30.2521008403361, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=127, Percent_Identity=33.8582677165354, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=33.9449541284404, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27073; Mature: 26941

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGNEGQTNGRKSYILVVEDDEALLMMLRYNLEKLGYEVGVAEDGEQALARVAVRRPDL
CCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCCCCE
MVLDWMLPGLSGLDVCRRLRDQEATRTLPIIMLTARAGEADHIRGLDIGADDYVTKPCSI
EEEEHHCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
EALQARIRALMRRAPPAEQMLSFADVTLDTVAHRAERNGRQLTLGPTEYRILEFFLRHPR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCHH
QVFSREDLLQRVWERGVHVEPRTVDVHIRRLRLILNGPGEADIVRTVRAAGYALDDPRP
HHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANGNEGQTNGRKSYILVVEDDEALLMMLRYNLEKLGYEVGVAEDGEQALARVAVRRPDL
CCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCCCCE
MVLDWMLPGLSGLDVCRRLRDQEATRTLPIIMLTARAGEADHIRGLDIGADDYVTKPCSI
EEEEHHCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
EALQARIRALMRRAPPAEQMLSFADVTLDTVAHRAERNGRQLTLGPTEYRILEFFLRHPR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCHH
QVFSREDLLQRVWERGVHVEPRTVDVHIRRLRLILNGPGEADIVRTVRAAGYALDDPRP
HHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]