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The map label for this gene is prpE [C]

Identifier: 162149512

GI number: 162149512

Start: 3831835

End: 3833595

Strand: Direct

Name: prpE [C]

Synonym: GDI_3750

Alternate gene names: 162149512

Gene position: 3831835-3833595 (Clockwise)

Preceding gene: 162149511

Following gene: 162149513

Centisome position: 97.15

GC content: 71.72

Gene sequence:

>1761_bases
ATGCTGCCGTGGACGACATACCAGGACATGCGCGATGCCGTGTCCGCCGATCCGGACGGGTTCTGGCTGGACGCGGCGCG
GCGCATCGCGTGGACGCGGCCGCCCGCCGCCGGGTGCGCGCCGCGCGCGGACGGGTGGCACGACTGGTTTCCCGGCGGGT
GGCTGAATACCTGCCACAACGCCGTGGACCGGCATGTCGCGGCCGGGCGGGGCGGGCAGGCGGCGCTGGTCTGGCAGTCC
TGCGCGACGAAGGAGCAGCAGATCGTCACCTATGCGGACCTTCAGCGCCGGGTCGCGGGGTTTGCCGGGGGGCTGCGCGC
GCTGGGCGTGGAAAAGGGCGACCGCGTGCTGATCGCGATGCCGACCCTGGTCGAGACCGCGATCGCCATGCTGGCCTGTG
CCCGGCTGGGGGCGGTGCATGTCGTGGTGTTCGCGGGATATGCGGCGCCGGAACTGGCGCGGCGGATCGACGACGTGGCG
CCGAAGATCGTCATCGTCGCCAGTTGCAGCTTCCAGGGCAGCGTGCCCGTGTCCTCGTACGACGTGCTGGCCGAGGCGCT
GGCCCTGGCCGCGCATGCGCCCGATGCCTGCGTCGTGCTGCAGCGTCCGGCCTGCCCGGCGCGGCTGGCGCCCGGCGATC
ATGACTTCCATGGGCTGGAGCGGCATGCGCCGGCGGCGCCCGTTCCGGTGAAATCGGAAGATCCGCTTTATATCCTGCAT
ACATCGGGAACGACGGGCGCGTCGAAGGGCATCGTCCGCGACAATGGCGGCCATGCGGTGGCGCTGGCGCTGTCCATGGA
GCTGATCTATGGCTGCCGGGCAGGGGATGTCTTCTTCACGACGTCGGACCTGGGCTGGGTGGTCGGCCATTCCTATGGCG
TCTATGCGCCGCTGCTGGGCGGCTGTACCAGCGTGATCATGGAAGGCGGGGCCTCGGCATCGTCGATCGCGGCGCTGTGT
CGTGCGCGGGGCGTGACCTGCCTGTTCACCACGCCCACGCAGTTGCGTCTGCTCCGCCAGGCGGAGCGGGGGGCGGCGGG
GGGCGCGCCGCTGGCATTGGCCCGCGTCTTCGTGGCCGGGGAATATGCCGATCCGACCCTGCTGGACTGGGCCCGGTCCT
ATTTCCGCTGCCCCGTCGCCAACCATTGGTGGCAGACGGAAACCGGGTGGAGCATCACGGCGCATTTCCTGGGCCTGCCG
GAAGAGGAAGCCCCGTCGGCCATGAACGATATCGGCCGGCCCGCGCCGGGCTTTAACGCCGCCGTCGGGGCGGGCGACGC
GTCCGGCGAGATCCTGCTGGCGCTGCCGCTTCCGCCGGGCTGCCTGGCGGGCGTGTGGATGGACGGGGGGATTCAGGCGC
CGCGTGCCTGCCTGGACGCCACCGGCCGCCATTACCGCACGTTCGACGAGGGGACGATCGGCGCGGACGGCGCGGTGCAC
ATGCTGGGCCGGTCCGACGACGTCATCAAGGTCGCGGGGCGGCGGATTTCCGGCGTGCAGATCGAAGCCATCATCGCGCG
CCATCCCGCCGTCCATGCCTGCGCCGTCGTCGCGGTAGCCGACGGGCTGAGGGGCCAGAGGCCGGTCGCCTATGTCGTGC
CCGACGCGTGGCCCGTGCCCCCCGCCCGGGCCGGCGAGATCATCGAGCAGGTCGGGCGCGGCCTGGGACGGTGGGTAGGC
ATCCGCGATGTCCGGTTCGTCGCGGCGCTTCCGACGACGCGATCGGGGAAGATCCTGCGCAGGCGGCTGGCCGTCGCCTG
A

Upstream 100 bases:

>100_bases
GGGGTCCGGGCGGTTCTCGGGCATCTCGCGCGATGCGGCGTGGCTGGCCGGGCAGATCACCGGCAAAGACATTCCCGTCG
CCTGAGCGGAGGCCGATGCC

Downstream 100 bases:

>100_bases
CGGGCGCCGGACGGGCCATGACGTTCCGGCAGGGCTGCCCCGGCCTGCGCGTCCGGGCCGGGGGACGGACGAAACGCCAT
GCCGCGCGTAGGAATACGAG

Product: propionate--CoA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MLPWTTYQDMRDAVSADPDGFWLDAARRIAWTRPPAAGCAPRADGWHDWFPGGWLNTCHNAVDRHVAAGRGGQAALVWQS
CATKEQQIVTYADLQRRVAGFAGGLRALGVEKGDRVLIAMPTLVETAIAMLACARLGAVHVVVFAGYAAPELARRIDDVA
PKIVIVASCSFQGSVPVSSYDVLAEALALAAHAPDACVVLQRPACPARLAPGDHDFHGLERHAPAAPVPVKSEDPLYILH
TSGTTGASKGIVRDNGGHAVALALSMELIYGCRAGDVFFTTSDLGWVVGHSYGVYAPLLGGCTSVIMEGGASASSIAALC
RARGVTCLFTTPTQLRLLRQAERGAAGGAPLALARVFVAGEYADPTLLDWARSYFRCPVANHWWQTETGWSITAHFLGLP
EEEAPSAMNDIGRPAPGFNAAVGAGDASGEILLALPLPPGCLAGVWMDGGIQAPRACLDATGRHYRTFDEGTIGADGAVH
MLGRSDDVIKVAGRRISGVQIEAIIARHPAVHACAVVAVADGLRGQRPVAYVVPDAWPVPPARAGEIIEQVGRGLGRWVG
IRDVRFVAALPTTRSGKILRRRLAVA

Sequences:

>Translated_586_residues
MLPWTTYQDMRDAVSADPDGFWLDAARRIAWTRPPAAGCAPRADGWHDWFPGGWLNTCHNAVDRHVAAGRGGQAALVWQS
CATKEQQIVTYADLQRRVAGFAGGLRALGVEKGDRVLIAMPTLVETAIAMLACARLGAVHVVVFAGYAAPELARRIDDVA
PKIVIVASCSFQGSVPVSSYDVLAEALALAAHAPDACVVLQRPACPARLAPGDHDFHGLERHAPAAPVPVKSEDPLYILH
TSGTTGASKGIVRDNGGHAVALALSMELIYGCRAGDVFFTTSDLGWVVGHSYGVYAPLLGGCTSVIMEGGASASSIAALC
RARGVTCLFTTPTQLRLLRQAERGAAGGAPLALARVFVAGEYADPTLLDWARSYFRCPVANHWWQTETGWSITAHFLGLP
EEEAPSAMNDIGRPAPGFNAAVGAGDASGEILLALPLPPGCLAGVWMDGGIQAPRACLDATGRHYRTFDEGTIGADGAVH
MLGRSDDVIKVAGRRISGVQIEAIIARHPAVHACAVVAVADGLRGQRPVAYVVPDAWPVPPARAGEIIEQVGRGLGRWVG
IRDVRFVAALPTTRSGKILRRRLAVA
>Mature_586_residues
MLPWTTYQDMRDAVSADPDGFWLDAARRIAWTRPPAAGCAPRADGWHDWFPGGWLNTCHNAVDRHVAAGRGGQAALVWQS
CATKEQQIVTYADLQRRVAGFAGGLRALGVEKGDRVLIAMPTLVETAIAMLACARLGAVHVVVFAGYAAPELARRIDDVA
PKIVIVASCSFQGSVPVSSYDVLAEALALAAHAPDACVVLQRPACPARLAPGDHDFHGLERHAPAAPVPVKSEDPLYILH
TSGTTGASKGIVRDNGGHAVALALSMELIYGCRAGDVFFTTSDLGWVVGHSYGVYAPLLGGCTSVIMEGGASASSIAALC
RARGVTCLFTTPTQLRLLRQAERGAAGGAPLALARVFVAGEYADPTLLDWARSYFRCPVANHWWQTETGWSITAHFLGLP
EEEAPSAMNDIGRPAPGFNAAVGAGDASGEILLALPLPPGCLAGVWMDGGIQAPRACLDATGRHYRTFDEGTIGADGAVH
MLGRSDDVIKVAGRRISGVQIEAIIARHPAVHACAVVAVADGLRGQRPVAYVVPDAWPVPPARAGEIIEQVGRGLGRWVG
IRDVRFVAALPTTRSGKILRRRLAVA

Specific function: Catalyzes The Synthesis Of Propionyl-CoA From Propionate And CoA. Also Converts Acetate To Acetyl-CoA But With A Lower Specific Activity (By Similarity). [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=600, Percent_Identity=40, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI8923896, Length=623, Percent_Identity=29.2134831460674, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI253970489, Length=636, Percent_Identity=29.0880503144654, Blast_Score=242, Evalue=8e-64,
Organism=Homo sapiens, GI28416953, Length=602, Percent_Identity=30.8970099667774, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI38505220, Length=552, Percent_Identity=26.6304347826087, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI42544132, Length=548, Percent_Identity=24.6350364963504, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI122937307, Length=495, Percent_Identity=25.6565656565657, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI115511026, Length=578, Percent_Identity=25.0865051903114, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI58082049, Length=543, Percent_Identity=23.572744014733, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI157311624, Length=543, Percent_Identity=23.3885819521179, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI157311622, Length=543, Percent_Identity=23.3885819521179, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786529, Length=590, Percent_Identity=37.7966101694915, Blast_Score=347, Evalue=2e-96,
Organism=Escherichia coli, GI1790505, Length=598, Percent_Identity=30.7692307692308, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI145693145, Length=514, Percent_Identity=25.875486381323, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1786801, Length=474, Percent_Identity=24.4725738396624, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786810, Length=525, Percent_Identity=23.2380952380952, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982997, Length=612, Percent_Identity=27.9411764705882, Blast_Score=214, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI71983001, Length=612, Percent_Identity=27.9411764705882, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17538037, Length=512, Percent_Identity=24.8046875, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=546, Percent_Identity=24.3589743589744, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=530, Percent_Identity=24.9056603773585, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323182, Length=613, Percent_Identity=31.6476345840131, Blast_Score=260, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6319264, Length=610, Percent_Identity=30.4918032786885, Blast_Score=246, Evalue=8e-66,
Organism=Drosophila melanogaster, GI21355225, Length=593, Percent_Identity=36.256323777403, Blast_Score=358, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24667955, Length=616, Percent_Identity=29.2207792207792, Blast_Score=252, Evalue=5e-67,
Organism=Drosophila melanogaster, GI62472339, Length=523, Percent_Identity=30.019120458891, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24667959, Length=492, Percent_Identity=29.0650406504065, Blast_Score=202, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.17 [C]

Molecular weight: Translated: 61774; Mature: 61774

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPWTTYQDMRDAVSADPDGFWLDAARRIAWTRPPAAGCAPRADGWHDWFPGGWLNTCHN
CCCCCCHHHHHHHHCCCCCCEEHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AVDRHVAAGRGGQAALVWQSCATKEQQIVTYADLQRRVAGFAGGLRALGVEKGDRVLIAM
HHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHCCHHEECCCCCCEEEEEC
PTLVETAIAMLACARLGAVHVVVFAGYAAPELARRIDDVAPKIVIVASCSFQGSVPVSSY
HHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHH
DVLAEALALAAHAPDACVVLQRPACPARLAPGDHDFHGLERHAPAAPVPVKSEDPLYILH
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEE
TSGTTGASKGIVRDNGGHAVALALSMELIYGCRAGDVFFTTSDLGWVVGHSYGVYAPLLG
CCCCCCCCCCCEECCCCCEEEEEEEHHHHHCCCCCCEEEEECCCCEECCCCCCHHHHHHH
GCTSVIMEGGASASSIAALCRARGVTCLFTTPTQLRLLRQAERGAAGGAPLALARVFVAG
HHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHCC
EYADPTLLDWARSYFRCPVANHWWQTETGWSITAHFLGLPEEEAPSAMNDIGRPAPGFNA
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHCCHHHHHCCCCCCCCCC
AVGAGDASGEILLALPLPPGCLAGVWMDGGIQAPRACLDATGRHYRTFDEGTIGADGAVH
EECCCCCCCCEEEEECCCCCHHEEEEECCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEE
MLGRSDDVIKVAGRRISGVQIEAIIARHPAVHACAVVAVADGLRGQRPVAYVVPDAWPVP
ECCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
PARAGEIIEQVGRGLGRWVGIRDVRFVAALPTTRSGKILRRRLAVA
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MLPWTTYQDMRDAVSADPDGFWLDAARRIAWTRPPAAGCAPRADGWHDWFPGGWLNTCHN
CCCCCCHHHHHHHHCCCCCCEEHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AVDRHVAAGRGGQAALVWQSCATKEQQIVTYADLQRRVAGFAGGLRALGVEKGDRVLIAM
HHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHCCHHEECCCCCCEEEEEC
PTLVETAIAMLACARLGAVHVVVFAGYAAPELARRIDDVAPKIVIVASCSFQGSVPVSSY
HHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHH
DVLAEALALAAHAPDACVVLQRPACPARLAPGDHDFHGLERHAPAAPVPVKSEDPLYILH
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEE
TSGTTGASKGIVRDNGGHAVALALSMELIYGCRAGDVFFTTSDLGWVVGHSYGVYAPLLG
CCCCCCCCCCCEECCCCCEEEEEEEHHHHHCCCCCCEEEEECCCCEECCCCCCHHHHHHH
GCTSVIMEGGASASSIAALCRARGVTCLFTTPTQLRLLRQAERGAAGGAPLALARVFVAG
HHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHCC
EYADPTLLDWARSYFRCPVANHWWQTETGWSITAHFLGLPEEEAPSAMNDIGRPAPGFNA
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHCCHHHHHCCCCCCCCCC
AVGAGDASGEILLALPLPPGCLAGVWMDGGIQAPRACLDATGRHYRTFDEGTIGADGAVH
EECCCCCCCCEEEEECCCCCHHEEEEECCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEE
MLGRSDDVIKVAGRRISGVQIEAIIARHPAVHACAVVAVADGLRGQRPVAYVVPDAWPVP
ECCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
PARAGEIIEQVGRGLGRWVGIRDVRFVAALPTTRSGKILRRRLAVA
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 1339433 [H]