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The map label for this gene is intA [H]

Identifier: 162149347

GI number: 162149347

Start: 3672861

End: 3674090

Strand: Direct

Name: intA [H]

Synonym: GDI_3581

Alternate gene names: 162149347

Gene position: 3672861-3674090 (Clockwise)

Preceding gene: 162149345

Following gene: 162149348

Centisome position: 93.12

GC content: 66.99

Gene sequence:

>1230_bases
ATGAGTCGGGTCGCGCCGCATCGTCTAACCGTCCGTCAGGTCGGCGCCCTCAAGGATGGGGCGCTCGCAGACGGCGGCAA
TCTCTGGGTGGTCGCGAAGGGTGGATCGAAGGTCTGGACGTTCCGCTACACCAGCCCGACGACCGGCAAGCGCCGCGAAA
TGGGCCTAGGACCGGTCCAGGACGTCAGCCTCGCGCAGGCCCGGCGTCTCGCGGCTGACGGCCGCCATCTGTTGATGGAC
GGCATCGACCCGATTGTGCAGCGGGACCGTGACCTGGCCGAACGCAAGAAAGAAAGCGGCGTGACTCTACAGACGGCTGC
CACTCGCTACATCGAGGAACAGACCCCGGGCTGGCGTGACCCGCGGGCTGCGAATATCTGGATCAGTTCGTTCGAGCGGC
TGGTATTCCCGCTCTGTGGAGACAAGGCCGTGGTCGGGATCGACACCGACGACGTGCTGGCCGTGCTGCGGCCAATCTGG
ACCACGAAGACCGAAACCGCCGACCGGCTGCGCGGGCGCCTTGAGCGCGTGCTGGACTATGCCCGGACACATGGCTGGCG
TGACGGCGACAATCCGGCTCGGTGGAAGGGGCACCTGGCGGCGATCCTGCCGAAGCCCTCGGCCGTCGCGAAGGTCGAGC
ACCATGCGGCTGTTGATCGGAAGGACATCGGCAAGGTGACGGCCGCCCTGGGCGCATCCCAGGGCGTTGCTGCCAAGGCC
GTCCGCTTCGCCTGCCTGACGGCTTCCCGATCTGGAGAGGTCCGATCGGCCGTCTGGTCGGAAATCGACATGAAGGCCGG
CATCTGGATCGTGCCGGCGCACAAGATGAAGGCCGGGCAGGAGCATCGCGTGCCGATGTCTGGATCCGCGCTGGCGATCT
TGAGAGAGGTCGAACCGCTGCGAGATCCGAAGGGCGCCGACTTCGTGTTCCCGGGCCAGAAGCGCGGCAAGACTCTGTCC
GATGTCGCGCTGTCCAAGGCCCTTCACCTGGCGGCCGGGACAAAGGCCGTCACCGTCCATGGCCTGCGATCCACCTTCCG
CGACTGGGCAGCCGAGGAAACCGACTATCCCCGCGAGGTGGCCGAGATGGCCCTGGCGCACGCGATCGGCGACAAGGTCG
AGGCAGCCTATCGCCGCGGCGACCTGTTCGAGAAGCGCCGGCAGATGATGGAGGATTGGGCGCGCTGGGTGCTGGCAGCG
GGCAACGGCGAAGCTGACCGGCAGTCGTAA

Upstream 100 bases:

>100_bases
GTTTCACATAGATATAGGGAAGCGCCTGTTCGCGGGCGTTCGCATCTGTTCCCGGTAAGCCCGGTTTCTAAGAGGGATAG
CAAGAGGGATAGCGAAGGTT

Downstream 100 bases:

>100_bases
GGGCCGGGAATCGACGGGGACATACTTTTTTCCGGTGTAACCGGTGTAACCGGTGTAACGGGACGCTCCGAATGGCGGGA
TGCTGCCATTTTTCTGAGGG

Product: phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSRVAPHRLTVRQVGALKDGALADGGNLWVVAKGGSKVWTFRYTSPTTGKRREMGLGPVQDVSLAQARRLAADGRHLLMD
GIDPIVQRDRDLAERKKESGVTLQTAATRYIEEQTPGWRDPRAANIWISSFERLVFPLCGDKAVVGIDTDDVLAVLRPIW
TTKTETADRLRGRLERVLDYARTHGWRDGDNPARWKGHLAAILPKPSAVAKVEHHAAVDRKDIGKVTAALGASQGVAAKA
VRFACLTASRSGEVRSAVWSEIDMKAGIWIVPAHKMKAGQEHRVPMSGSALAILREVEPLRDPKGADFVFPGQKRGKTLS
DVALSKALHLAAGTKAVTVHGLRSTFRDWAAEETDYPREVAEMALAHAIGDKVEAAYRRGDLFEKRRQMMEDWARWVLAA
GNGEADRQS

Sequences:

>Translated_409_residues
MSRVAPHRLTVRQVGALKDGALADGGNLWVVAKGGSKVWTFRYTSPTTGKRREMGLGPVQDVSLAQARRLAADGRHLLMD
GIDPIVQRDRDLAERKKESGVTLQTAATRYIEEQTPGWRDPRAANIWISSFERLVFPLCGDKAVVGIDTDDVLAVLRPIW
TTKTETADRLRGRLERVLDYARTHGWRDGDNPARWKGHLAAILPKPSAVAKVEHHAAVDRKDIGKVTAALGASQGVAAKA
VRFACLTASRSGEVRSAVWSEIDMKAGIWIVPAHKMKAGQEHRVPMSGSALAILREVEPLRDPKGADFVFPGQKRGKTLS
DVALSKALHLAAGTKAVTVHGLRSTFRDWAAEETDYPREVAEMALAHAIGDKVEAAYRRGDLFEKRRQMMEDWARWVLAA
GNGEADRQS
>Mature_408_residues
SRVAPHRLTVRQVGALKDGALADGGNLWVVAKGGSKVWTFRYTSPTTGKRREMGLGPVQDVSLAQARRLAADGRHLLMDG
IDPIVQRDRDLAERKKESGVTLQTAATRYIEEQTPGWRDPRAANIWISSFERLVFPLCGDKAVVGIDTDDVLAVLRPIWT
TKTETADRLRGRLERVLDYARTHGWRDGDNPARWKGHLAAILPKPSAVAKVEHHAAVDRKDIGKVTAALGASQGVAAKAV
RFACLTASRSGEVRSAVWSEIDMKAGIWIVPAHKMKAGQEHRVPMSGSALAILREVEPLRDPKGADFVFPGQKRGKTLSD
VALSKALHLAAGTKAVTVHGLRSTFRDWAAEETDYPREVAEMALAHAIGDKVEAAYRRGDLFEKRRQMMEDWARWVLAAG
NGEADRQS

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=398, Percent_Identity=31.6582914572864, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI145693166, Length=382, Percent_Identity=29.8429319371728, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788690, Length=406, Percent_Identity=27.0935960591133, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 44933; Mature: 44802

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVAPHRLTVRQVGALKDGALADGGNLWVVAKGGSKVWTFRYTSPTTGKRREMGLGPVQ
CCCCCCCHHHHHHHCCCCCCCEECCCCEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCC
DVSLAQARRLAADGRHLLMDGIDPIVQRDRDLAERKKESGVTLQTAATRYIEEQTPGWRD
HHHHHHHHHHHHCCCEEEHHCCCHHHHCCHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCC
PRAANIWISSFERLVFPLCGDKAVVGIDTDDVLAVLRPIWTTKTETADRLRGRLERVLDY
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ARTHGWRDGDNPARWKGHLAAILPKPSAVAKVEHHAAVDRKDIGKVTAALGASQGVAAKA
HHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
VRFACLTASRSGEVRSAVWSEIDMKAGIWIVPAHKMKAGQEHRVPMSGSALAILREVEPL
HHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCCCCHHHHHHHHHHC
RDPKGADFVFPGQKRGKTLSDVALSKALHLAAGTKAVTVHGLRSTFRDWAAEETDYPREV
CCCCCCCEECCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCHHHH
AEMALAHAIGDKVEAAYRRGDLFEKRRQMMEDWARWVLAAGNGEADRQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
>Mature Secondary Structure 
SRVAPHRLTVRQVGALKDGALADGGNLWVVAKGGSKVWTFRYTSPTTGKRREMGLGPVQ
CCCCCCHHHHHHHCCCCCCCEECCCCEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCC
DVSLAQARRLAADGRHLLMDGIDPIVQRDRDLAERKKESGVTLQTAATRYIEEQTPGWRD
HHHHHHHHHHHHCCCEEEHHCCCHHHHCCHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCC
PRAANIWISSFERLVFPLCGDKAVVGIDTDDVLAVLRPIWTTKTETADRLRGRLERVLDY
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ARTHGWRDGDNPARWKGHLAAILPKPSAVAKVEHHAAVDRKDIGKVTAALGASQGVAAKA
HHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
VRFACLTASRSGEVRSAVWSEIDMKAGIWIVPAHKMKAGQEHRVPMSGSALAILREVEPL
HHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCCCCHHHHHHHHHHC
RDPKGADFVFPGQKRGKTLSDVALSKALHLAAGTKAVTVHGLRSTFRDWAAEETDYPREV
CCCCCCCEECCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCHHHH
AEMALAHAIGDKVEAAYRRGDLFEKRRQMMEDWARWVLAAGNGEADRQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]