Click here to switch to the map view.

The map label for this gene is tkrA [H]

Identifier: 162149202

GI number: 162149202

Start: 3533785

End: 3534759

Strand: Direct

Name: tkrA [H]

Synonym: GDI_3432

Alternate gene names: 162149202

Gene position: 3533785-3534759 (Clockwise)

Preceding gene: 162149196

Following gene: 162149203

Centisome position: 89.6

GC content: 72.1

Gene sequence:

>975_bases
ATGTCGCGGACGCGCCCCCGCCTGATCCATTCCCAGCGCCTGCCGGACGCCGTCGCCGCGCGGATCGCGCGCGACTACGA
CACCCCGCCCGCGCCCGACCACAAGCTGACGGCGGACGAACTGGTCGCGCTGGCGCGCGATTTCCGCCCCGACGCGGTGC
TGGTCACCAGCAGCACCCCGGTTTCGGCCGCCACGGTCGCCGCCCTGCCGGACAGCGTGCGCGTCATCGCCACCATCAGC
GTGGGCACCGATCACCTGGACATTCCCGCCATCGTCGCGCGCGGGTGGGCGCTGACCTACACGCCCGACGTGCTGACGGA
CTGCAATGCCGACCTGACGATGATGCTGATCCTGGCCGCCGCCCGGCGCGGCGCGGAGTATCTGTCGGTCATGCGCGGGG
GGTGGGGCCGGTCGCTGGGCATGGAGGAAATGCTGGGCACCCGCGTCACGGGCAAGACCCTGGGCATCATCGGCATGGGG
CGGATCGGCCGCGCCGTCGCCCGGCGGGCGCGGGGCTTCGACATGAAGGTCCTGTATTCCAACCGGCGCCGCCTGGCGCC
GGACCTGGAACAGGGCGCCACCTATTTCTCCGACATGCGCGACATGCTGCCGCACTGCGATATCCTGACCCTGCACATGC
CCGGCAGCAAGGGCGCCCCCCCGGTCATTACCGCCGACCTGCTGGCGCGACTGCCCCGGGGCGCGATCTTCGTCAACGCG
GCGCGCGGCAGCCTGGTGGACGAGGACGCGCTGATCGCCGCCCTGTCCGACGGGCACCTGGCGGCGGCCGGGCTGGACGT
CTACCGCAACGAACCGCACCCCGACCCGCGCTTCCTGGCGCTGTCGAACGTGTTCCTGACCCCGCACATGGGCAGCGCCA
CGCTGGAAACCCGCACCGGCATGGGCATGCTGGCGCTGGACAATATCGACGCGGTGCTGGCCGGCGGCCCGGCCGTCACC
CCCGTTCCCGTCTGA

Upstream 100 bases:

>100_bases
CGCCGCATCCGGTGTCTGGACACGGCCCGGTCATGCCCGGCAATGATTGCATCCTGATACAGCAGCGGGATCCACCGCAC
CTGTCAAAAAAGGTCCATCC

Downstream 100 bases:

>100_bases
CACGGAGGCCGCCATGCAGCCCCGCCTGACCTTCCTGTTCGCCTGCCTGACCGGCTGCCTGGCCGCCATCGCCCTGATGG
TGGCGGCCCCCCATTACGCG

Product: 2-ketogluconate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSRTRPRLIHSQRLPDAVAARIARDYDTPPAPDHKLTADELVALARDFRPDAVLVTSSTPVSAATVAALPDSVRVIATIS
VGTDHLDIPAIVARGWALTYTPDVLTDCNADLTMMLILAAARRGAEYLSVMRGGWGRSLGMEEMLGTRVTGKTLGIIGMG
RIGRAVARRARGFDMKVLYSNRRRLAPDLEQGATYFSDMRDMLPHCDILTLHMPGSKGAPPVITADLLARLPRGAIFVNA
ARGSLVDEDALIAALSDGHLAAAGLDVYRNEPHPDPRFLALSNVFLTPHMGSATLETRTGMGMLALDNIDAVLAGGPAVT
PVPV

Sequences:

>Translated_324_residues
MSRTRPRLIHSQRLPDAVAARIARDYDTPPAPDHKLTADELVALARDFRPDAVLVTSSTPVSAATVAALPDSVRVIATIS
VGTDHLDIPAIVARGWALTYTPDVLTDCNADLTMMLILAAARRGAEYLSVMRGGWGRSLGMEEMLGTRVTGKTLGIIGMG
RIGRAVARRARGFDMKVLYSNRRRLAPDLEQGATYFSDMRDMLPHCDILTLHMPGSKGAPPVITADLLARLPRGAIFVNA
ARGSLVDEDALIAALSDGHLAAAGLDVYRNEPHPDPRFLALSNVFLTPHMGSATLETRTGMGMLALDNIDAVLAGGPAVT
PVPV
>Mature_323_residues
SRTRPRLIHSQRLPDAVAARIARDYDTPPAPDHKLTADELVALARDFRPDAVLVTSSTPVSAATVAALPDSVRVIATISV
GTDHLDIPAIVARGWALTYTPDVLTDCNADLTMMLILAAARRGAEYLSVMRGGWGRSLGMEEMLGTRVTGKTLGIIGMGR
IGRAVARRARGFDMKVLYSNRRRLAPDLEQGATYFSDMRDMLPHCDILTLHMPGSKGAPPVITADLLARLPRGAIFVNAA
RGSLVDEDALIAALSDGHLAAAGLDVYRNEPHPDPRFLALSNVFLTPHMGSATLETRTGMGMLALDNIDAVLAGGPAVTP
VPV

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=252, Percent_Identity=38.4920634920635, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI23308577, Length=302, Percent_Identity=31.1258278145695, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI61743967, Length=240, Percent_Identity=33.75, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4557497, Length=240, Percent_Identity=33.75, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI145580578, Length=240, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI4557499, Length=240, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI145580575, Length=241, Percent_Identity=33.195020746888, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI87082289, Length=314, Percent_Identity=39.171974522293, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789279, Length=235, Percent_Identity=31.063829787234, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787645, Length=257, Percent_Identity=26.8482490272374, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081824, Length=144, Percent_Identity=32.6388888888889, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788660, Length=252, Percent_Identity=28.1746031746032, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=279, Percent_Identity=31.8996415770609, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25147481, Length=251, Percent_Identity=29.8804780876494, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=261, Percent_Identity=32.9501915708812, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322116, Length=324, Percent_Identity=27.4691358024691, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320925, Length=302, Percent_Identity=27.4834437086093, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325144, Length=172, Percent_Identity=29.0697674418605, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321253, Length=233, Percent_Identity=27.0386266094421, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=272, Percent_Identity=24.2647058823529, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45551003, Length=274, Percent_Identity=35.036496350365, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24585514, Length=274, Percent_Identity=35.036496350365, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28574282, Length=274, Percent_Identity=35.036496350365, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45552429, Length=274, Percent_Identity=35.036496350365, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28574284, Length=274, Percent_Identity=35.036496350365, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28574286, Length=254, Percent_Identity=36.6141732283465, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24585516, Length=274, Percent_Identity=32.4817518248175, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI28571528, Length=275, Percent_Identity=35.2727272727273, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19921140, Length=290, Percent_Identity=30.6896551724138, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI62472511, Length=226, Percent_Identity=34.070796460177, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646446, Length=228, Percent_Identity=33.7719298245614, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646448, Length=228, Percent_Identity=33.7719298245614, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646452, Length=228, Percent_Identity=33.7719298245614, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646450, Length=228, Percent_Identity=33.7719298245614, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34568; Mature: 34437

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTRPRLIHSQRLPDAVAARIARDYDTPPAPDHKLTADELVALARDFRPDAVLVTSSTP
CCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCC
VSAATVAALPDSVRVIATISVGTDHLDIPAIVARGWALTYTPDVLTDCNADLTMMLILAA
CCHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHCCEEEEECCHHHHCCCCCHHHHHHHHH
ARRGAEYLSVMRGGWGRSLGMEEMLGTRVTGKTLGIIGMGRIGRAVARRARGFDMKVLYS
HHCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEC
NRRRLAPDLEQGATYFSDMRDMLPHCDILTLHMPGSKGAPPVITADLLARLPRGAIFVNA
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHHCCCCEEEEEE
ARGSLVDEDALIAALSDGHLAAAGLDVYRNEPHPDPRFLALSNVFLTPHMGSATLETRTG
CCCCCCCCCCEEEEECCCCEEEECHHHHCCCCCCCCCEEEEEEEEECCCCCCCEEECCCC
MGMLALDNIDAVLAGGPAVTPVPV
CCEEEECCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
SRTRPRLIHSQRLPDAVAARIARDYDTPPAPDHKLTADELVALARDFRPDAVLVTSSTP
CCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCC
VSAATVAALPDSVRVIATISVGTDHLDIPAIVARGWALTYTPDVLTDCNADLTMMLILAA
CCHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHCCEEEEECCHHHHCCCCCHHHHHHHHH
ARRGAEYLSVMRGGWGRSLGMEEMLGTRVTGKTLGIIGMGRIGRAVARRARGFDMKVLYS
HHCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEC
NRRRLAPDLEQGATYFSDMRDMLPHCDILTLHMPGSKGAPPVITADLLARLPRGAIFVNA
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHHCCCCEEEEEE
ARGSLVDEDALIAALSDGHLAAAGLDVYRNEPHPDPRFLALSNVFLTPHMGSATLETRTG
CCCCCCCCCCEEEEECCCCEEEECHHHHCCCCCCCCCEEEEEEEEECCCCCCCEEECCCC
MGMLALDNIDAVLAGGPAVTPVPV
CCEEEECCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA