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The map label for this gene is istB [H]

Identifier: 162148915

GI number: 162148915

Start: 3234867

End: 3235646

Strand: Direct

Name: istB [H]

Synonym: GDI_3145

Alternate gene names: 162148915

Gene position: 3234867-3235646 (Clockwise)

Preceding gene: 162148914

Following gene: 162148918

Centisome position: 82.02

GC content: 60.77

Gene sequence:

>780_bases
ATGCGTCATGATCCCGCGGCCGGTGCCCTCGTCGTCATGCTGCGCGGCCTGCGGATGTATGGCATGGCCCAGGCCACGGC
CGAACTGACCGAACAGGGTGCGCCGGCATTCGAGGCCGCCATCCCCGTCCTCTCCCAGCTTTTGAAGGCGGAACTCGCCG
AGCGAAAGGTGCGCTCCATCGCCTATCAAACCAAGACTGCCAGGTTCCCGGCCTACAAAGATTTGGCAGGGTTCGATTTC
TCGGCCGCCGAGGTCAACGAGGCCATGGTCCGTCAACTCCATGCCGGGGATTTCATCGACCGTGCCGACAACGTCGTCCT
CATTGGTGGACCAGGAACCGGCAAGACCCATCTGGCCACCGCACTTGCCGTGCAGGCGATCGAACATCACCGCAAGAAGA
TACGGTTCTGGTCCACGGTCGACCTCGTCAACGCCCTCGAACAGGAAAAAACCGCCAATCGCGCAGGACAGATCGCGGAA
CGTCTCCTGCGCCTCGATCTCGTGATCCTGGACGAACTTGGCTATTTGCCGTTCAGCGCATCAGGCGGTGCCCTGCTGTT
CCATCTCCTCAGCCGTCTCTACGAGCGCACCAGCGTCATCATCACCACCAATCTGAGCTTCAGCGAATGGGGCGAAGTCT
TCGGTGATCCCAAAATGACGACAGCCCTGCTCGATCGCCTTACCCACCACTGTCATATCCTCGAAACCGGAAATGACAGC
TACCGGTTCCGCGCAAGCTCCGCCGCCCCCAGGAACCGGAAGGAAAAGGCAACCGCTTGA

Upstream 100 bases:

>100_bases
ACAGGAGCACCCAGGCTCGACGCGCCGCAGGCATTGGTGCTCGAACGCGAGCCTCAGGCCGATACCGGACGGTATGACGC
CCTGCGCGGGGAGGCCCGTC

Downstream 100 bases:

>100_bases
CCAGCCCATAAAACATAGCAGAGATAACCAAAGGCCGGGTCAGTTCTCGATGAAAACCCAGGGTCAAATCTCAGCAGAAA
TTAACAATAGACGGATCGGC

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRHDPAAGALVVMLRGLRMYGMAQATAELTEQGAPAFEAAIPVLSQLLKAELAERKVRSIAYQTKTARFPAYKDLAGFDF
SAAEVNEAMVRQLHAGDFIDRADNVVLIGGPGTGKTHLATALAVQAIEHHRKKIRFWSTVDLVNALEQEKTANRAGQIAE
RLLRLDLVILDELGYLPFSASGGALLFHLLSRLYERTSVIITTNLSFSEWGEVFGDPKMTTALLDRLTHHCHILETGNDS
YRFRASSAAPRNRKEKATA

Sequences:

>Translated_259_residues
MRHDPAAGALVVMLRGLRMYGMAQATAELTEQGAPAFEAAIPVLSQLLKAELAERKVRSIAYQTKTARFPAYKDLAGFDF
SAAEVNEAMVRQLHAGDFIDRADNVVLIGGPGTGKTHLATALAVQAIEHHRKKIRFWSTVDLVNALEQEKTANRAGQIAE
RLLRLDLVILDELGYLPFSASGGALLFHLLSRLYERTSVIITTNLSFSEWGEVFGDPKMTTALLDRLTHHCHILETGNDS
YRFRASSAAPRNRKEKATA
>Mature_259_residues
MRHDPAAGALVVMLRGLRMYGMAQATAELTEQGAPAFEAAIPVLSQLLKAELAERKVRSIAYQTKTARFPAYKDLAGFDF
SAAEVNEAMVRQLHAGDFIDRADNVVLIGGPGTGKTHLATALAVQAIEHHRKKIRFWSTVDLVNALEQEKTANRAGQIAE
RLLRLDLVILDELGYLPFSASGGALLFHLLSRLYERTSVIITTNLSFSEWGEVFGDPKMTTALLDRLTHHCHILETGNDS
YRFRASSAAPRNRKEKATA

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 28528; Mature: 28528

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHDPAAGALVVMLRGLRMYGMAQATAELTEQGAPAFEAAIPVLSQLLKAELAERKVRSI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYQTKTARFPAYKDLAGFDFSAAEVNEAMVRQLHAGDFIDRADNVVLIGGPGTGKTHLAT
HHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCHHHHHH
ALAVQAIEHHRKKIRFWSTVDLVNALEQEKTANRAGQIAERLLRLDLVILDELGYLPFSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGGALLFHLLSRLYERTSVIITTNLSFSEWGEVFGDPKMTTALLDRLTHHCHILETGNDS
CCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCCC
YRFRASSAAPRNRKEKATA
EEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRHDPAAGALVVMLRGLRMYGMAQATAELTEQGAPAFEAAIPVLSQLLKAELAERKVRSI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYQTKTARFPAYKDLAGFDFSAAEVNEAMVRQLHAGDFIDRADNVVLIGGPGTGKTHLAT
HHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCHHHHHH
ALAVQAIEHHRKKIRFWSTVDLVNALEQEKTANRAGQIAERLLRLDLVILDELGYLPFSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGGALLFHLLSRLYERTSVIITTNLSFSEWGEVFGDPKMTTALLDRLTHHCHILETGNDS
CCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCCC
YRFRASSAAPRNRKEKATA
EEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]