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The map label for this gene is pbuE [H]

Identifier: 162146804

GI number: 162146804

Start: 1001646

End: 1002785

Strand: Reverse

Name: pbuE [H]

Synonym: GDI_0986

Alternate gene names: 162146804

Gene position: 1002785-1001646 (Counterclockwise)

Preceding gene: 162146805

Following gene: 162146800

Centisome position: 25.42

GC content: 61.23

Gene sequence:

>1140_bases
ATGATCGATCCTCGTATTCTGCTCCTGGCCTTGGGCACCTTCGCGATTGGCACGGAGGGTTATGCGATCGCCGGCCTGTT
GCCGATGATCGCGAGCGACCTACGCATCAGCGTCGCTCAATGCGGCCAGATGGTGACGGTCTTCGCGCTGGCATACGCGC
TGGGTGGTCCTGTTCTTGTTGCCAGATTCGGAGCGATGGCCCGTAAGCGATTGATCATTGGCTCGCTTCTTGTTTTTGGG
CTCACCAACCTGCTGGCGGCTTTCGCACGTTCGTGGGAAGTGTTGGCGGTGGCGCGGGTGTTAGCGGGTTTTGCCGCCGC
CTTATTTATGGCGCCCGCTAGCGCCGCCGCCGTGGCCTTGTCTCCTCCGAATCGGAGAGGCCATGCGCTCTCCATCACGG
CTACTGGAAACGCCTTGGCTCTGGCGGCAGGAGCTCCGCTGGGCACCATGGCTGGTGCGCTATGGGGATGGCCGGGAGCT
TTCATCCTCGTGGCGGTGCTGAGTGGAACTGCGGCTGTCGGACTAGGGCTGATGTTGCCTGCTTTGCCTTCGTCACCTGC
GGTGCCATTTCGCACGCGCTTCGCGCTTTTGCGCCGCTCGTCCGTGCGGAAGGGCTTGGCTACCTCCTTCGGAATGTTCG
TATGTGCCTTGACAGCCTACACCTATCTGACGCCGCTGGCTGCACAGGCTGCGGGCATGCAGCCACCGCAAGTCGCCGCC
CTCATGGTTGTGTTTGGCATCTTGGCCACGACTGCAGGCCTAGCGGCTGGACATACGATCAGTCGGTTCGGGGCAAACCG
CGTGGTTAGGGGTTCCCTTGTAATTATGGCAGCCCTCTTCTGCCTGCTCGGTGTTCTGGCGATTGAATGCCAGGAGGGGC
CGGTTTCCGCTGGGCTGATGTACCTGCTCGTCGCCTGCTGGGGAGGCGCATGGTGGAGTGGCGGTATTGCTCAGCAACAC
CGGATGACGGATTTGGCACCGGATCAGCATGCGGCCGTTCTCTCGTTGCATTATGCTATGCAGTTCCTCGGCATTGCTGC
CGGCGGTGCGCTTGGCGGCCTACTTCTGAGCGTCGTTAGCACATCCGCTATCGCCTTTGGCGCTGCCGCTGTCGGTATGA
TCACGACGATGTTTTTATAG

Upstream 100 bases:

>100_bases
GTGGGAAAAAGAACCATCACGGTTCATACATAATCCGCACCACCAAATCCCGGGACCAAACATCTAGCTAACGGTACAAC
GATCGTTATACAATAGGACC

Downstream 100 bases:

>100_bases
AACCAAATCGCGGACCAGCTTATTGCGATATCGAAATTCGACTGAGAAATGGCAGCTATCAACCAGCTCATGCCGTTATG
TTCTGTAAAGCCAGCAATAC

Product: major facilitator transporter superfamily protein

Products: arabinose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MIDPRILLLALGTFAIGTEGYAIAGLLPMIASDLRISVAQCGQMVTVFALAYALGGPVLVARFGAMARKRLIIGSLLVFG
LTNLLAAFARSWEVLAVARVLAGFAAALFMAPASAAAVALSPPNRRGHALSITATGNALALAAGAPLGTMAGALWGWPGA
FILVAVLSGTAAVGLGLMLPALPSSPAVPFRTRFALLRRSSVRKGLATSFGMFVCALTAYTYLTPLAAQAAGMQPPQVAA
LMVVFGILATTAGLAAGHTISRFGANRVVRGSLVIMAALFCLLGVLAIECQEGPVSAGLMYLLVACWGGAWWSGGIAQQH
RMTDLAPDQHAAVLSLHYAMQFLGIAAGGALGGLLLSVVSTSAIAFGAAAVGMITTMFL

Sequences:

>Translated_379_residues
MIDPRILLLALGTFAIGTEGYAIAGLLPMIASDLRISVAQCGQMVTVFALAYALGGPVLVARFGAMARKRLIIGSLLVFG
LTNLLAAFARSWEVLAVARVLAGFAAALFMAPASAAAVALSPPNRRGHALSITATGNALALAAGAPLGTMAGALWGWPGA
FILVAVLSGTAAVGLGLMLPALPSSPAVPFRTRFALLRRSSVRKGLATSFGMFVCALTAYTYLTPLAAQAAGMQPPQVAA
LMVVFGILATTAGLAAGHTISRFGANRVVRGSLVIMAALFCLLGVLAIECQEGPVSAGLMYLLVACWGGAWWSGGIAQQH
RMTDLAPDQHAAVLSLHYAMQFLGIAAGGALGGLLLSVVSTSAIAFGAAAVGMITTMFL
>Mature_379_residues
MIDPRILLLALGTFAIGTEGYAIAGLLPMIASDLRISVAQCGQMVTVFALAYALGGPVLVARFGAMARKRLIIGSLLVFG
LTNLLAAFARSWEVLAVARVLAGFAAALFMAPASAAAVALSPPNRRGHALSITATGNALALAAGAPLGTMAGALWGWPGA
FILVAVLSGTAAVGLGLMLPALPSSPAVPFRTRFALLRRSSVRKGLATSFGMFVCALTAYTYLTPLAAQAAGMQPPQVAA
LMVVFGILATTAGLAAGHTISRFGANRVVRGSLVIMAALFCLLGVLAIECQEGPVSAGLMYLLVACWGGAWWSGGIAQQH
RMTDLAPDQHAAVLSLHYAMQFLGIAAGGALGGLLLSVVSTSAIAFGAAAVGMITTMFL

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787808, Length=228, Percent_Identity=29.8245614035088, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787947, Length=272, Percent_Identity=27.9411764705882, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI145693198, Length=255, Percent_Identity=29.8039215686275, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786595, Length=282, Percent_Identity=23.4042553191489, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 38566; Mature: 38566

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS00435 PEROXIDASE_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPRILLLALGTFAIGTEGYAIAGLLPMIASDLRISVAQCGQMVTVFALAYALGGPVLV
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARFGAMARKRLIIGSLLVFGLTNLLAAFARSWEVLAVARVLAGFAAALFMAPASAAAVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
SPPNRRGHALSITATGNALALAAGAPLGTMAGALWGWPGAFILVAVLSGTAAVGLGLMLP
CCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHC
ALPSSPAVPFRTRFALLRRSSVRKGLATSFGMFVCALTAYTYLTPLAAQAAGMQPPQVAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LMVVFGILATTAGLAAGHTISRFGANRVVRGSLVIMAALFCLLGVLAIECQEGPVSAGLM
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
YLLVACWGGAWWSGGIAQQHRMTDLAPDQHAAVLSLHYAMQFLGIAAGGALGGLLLSVVS
HHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TSAIAFGAAAVGMITTMFL
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDPRILLLALGTFAIGTEGYAIAGLLPMIASDLRISVAQCGQMVTVFALAYALGGPVLV
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARFGAMARKRLIIGSLLVFGLTNLLAAFARSWEVLAVARVLAGFAAALFMAPASAAAVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
SPPNRRGHALSITATGNALALAAGAPLGTMAGALWGWPGAFILVAVLSGTAAVGLGLMLP
CCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHC
ALPSSPAVPFRTRFALLRRSSVRKGLATSFGMFVCALTAYTYLTPLAAQAAGMQPPQVAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LMVVFGILATTAGLAAGHTISRFGANRVVRGSLVIMAALFCLLGVLAIECQEGPVSAGLM
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
YLLVACWGGAWWSGGIAQQHRMTDLAPDQHAAVLSLHYAMQFLGIAAGGALGGLLLSVVS
HHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TSAIAFGAAAVGMITTMFL
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: arabinose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: arabinose [Cytoplasm] + Proton [Periplasm] = arabinose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]