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The map label for this gene is hyuA

Identifier: 161984858

GI number: 161984858

Start: 3112720

End: 3114105

Strand: Reverse

Name: hyuA

Synonym: SBO_3111

Alternate gene names: 161984858

Gene position: 3114105-3112720 (Counterclockwise)

Preceding gene: 82545497

Following gene: 82545495

Centisome position: 68.9

GC content: 52.96

Gene sequence:

>1386_bases
ATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCTGATTGAAAGCGGGAT
TGTTCGCCAGTTGGGCAACAATATTTCGCCGCAGCTCCCGTATGAAGAAATTGATGCCACTGGCTGTTACGTTTTCCCAG
GCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTACCGGTACCCGC
GCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCATATGGGATTTGGCCCAAACGGCTGTCGGTTACGCCATCAACT
GGAGGTTTATCGTGGTTATGCCGCCCATAAAGCGGTCATCGATTACAGCTTTCACGGTGTGATCCAGCACATTAATCACG
CAATCCTCGACGAAATCCCGATGATGGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTC
AACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGTGCACCCGGAAAATGATGC
GGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGG
AATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTGGCACAAATTGCCGGTAACGCCCCGCTCTATATCGTGCACCTG
TCTAACGGCTTAGGTCTGGATTATCTGCGTCTTGCCCGTGCGAATCACCAGCCAGTCTGGGTTGAAACCTGCCCACAATA
TCTCCTGTTGGACGAACGCAGTTACGATACAGAAGATGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCG
AGCAGGACAAACTGTGGTGTGGCATCAGCGATGGTGCGATTGACGTGGTGGCGACCGATCACTGCACCTTCTCGATGGCT
CAACGCCTGCAAATTTCTAAAGGCGATTTCAGTCGCTGCCCAAATGGCTTACCCGGTGTGGAAAACCGCATGCAGTTACT
GTTTTCCAGTGGCGTGATGACGGGACGTATAACACCGGAACGCTTTGTTGAATTAACCAGCGCAATGCCCGCCAGGCTGT
TTGGCCTGTGGCCGCAAAAAGGATTATTAGCGCCCGGTTCCGATGGCGACGTGGTGATTATCGACCCACGTCAGAGCCAA
CAAATTCAGCATCGCCATCTCCACGACAACGCCGACTACTCGCCATGGGAGGGTTTTACCTGTCAGGGCGCGATTGTCAG
AACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCA
AACCGTTTGTCCCTCCCGTGCTCTAA

Upstream 100 bases:

>100_bases
GCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCG
GAGGGTTTTTGGAGTTTGCT

Downstream 100 bases:

>100_bases
CCACTGTCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGG
AATGCGCTGGGCGATGACCT

Product: phenylhydantoinase

Products: NA

Alternate protein names: Hydantoin-utilizing enzyme hyuA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTR
AAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMA
QRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQ
QIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL

Sequences:

>Translated_461_residues
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTR
AAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMA
QRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQ
QIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
>Mature_461_residues
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTR
AAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMA
QRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQ
QIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives with an aromatic side chains at the 5'-position. Has no activity on dihydropyrimidines. The physiological function is unknown

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily

Homologues:

Organism=Homo sapiens, GI4503375, Length=466, Percent_Identity=36.6952789699571, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI4503379, Length=462, Percent_Identity=35.0649350649351, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI4503377, Length=462, Percent_Identity=35.4978354978355, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI4503051, Length=462, Percent_Identity=35.4978354978355, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI62422571, Length=462, Percent_Identity=35.4978354978355, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI19923821, Length=463, Percent_Identity=34.341252699784, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI190194363, Length=462, Percent_Identity=34.1991341991342, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI18105007, Length=405, Percent_Identity=23.7037037037037, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI87082175, Length=461, Percent_Identity=99.7830802603037, Blast_Score=957, Evalue=0.0,
Organism=Escherichia coli, GI1786722, Length=444, Percent_Identity=29.7297297297297, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71989490, Length=467, Percent_Identity=36.4025695931477, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17539558, Length=463, Percent_Identity=35.85313174946, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI86575075, Length=473, Percent_Identity=28.5412262156448, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI193204318, Length=409, Percent_Identity=25.1833740831296, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322218, Length=423, Percent_Identity=27.6595744680851, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI221377917, Length=481, Percent_Identity=35.7588357588358, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI17137462, Length=468, Percent_Identity=35.6837606837607, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24644287, Length=310, Percent_Identity=36.4516129032258, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24644289, Length=235, Percent_Identity=38.7234042553192, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24642586, Length=408, Percent_Identity=25.7352941176471, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18859883, Length=457, Percent_Identity=24.72647702407, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHYDA_ECO24 (A7ZQY1)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001464210.1
- ProteinModelPortal:   A7ZQY1
- SMR:   A7ZQY1
- STRING:   A7ZQY1
- EnsemblBacteria:   EBESCT00000021147
- GeneID:   5587404
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_3198
- eggNOG:   COG0044
- GeneTree:   EBGT00050000008279
- HOGENOM:   HBG724623
- OMA:   MTGRISP
- ProtClustDB:   PRK08323
- BioCyc:   ECOL331111:ECE24377A_3198-MONOMER
- HAMAP:   MF_01644
- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059
- TIGRFAMs:   TIGR02033

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: 3.-.-.- [C]

Molecular weight: Translated: 51044; Mature: 51044

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: BINDING 156-156 BINDING 286-286 BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHT
CEEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCEEEEE
HFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVI
EEEEEEECCCCCHHHHCCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHEEE
DYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESGALTT
EHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCEEE
VHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
ECCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWC
CCCCCHHHHHHHHCCCCEEEHCCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHCCEEE
GISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPE
EECCCEEEEEEECCHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCEEECCCHH
RFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPWEGFT
HHHHHHHCCHHHHHCCCCCCCEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCEE
CQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
ECHHHHHHHCCCCEEEECCEECCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHT
CEEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCEEEEE
HFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVI
EEEEEEECCCCCHHHHCCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHEEE
DYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESGALTT
EHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCEEE
VHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
ECCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWC
CCCCCHHHHHHHHCCCCEEEHCCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHCCEEE
GISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPE
EECCCEEEEEEECCHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCEEECCCHH
RFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPWEGFT
HHHHHHHCCHHHHHCCCCCCCEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCEE
CQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
ECHHHHHHHCCCCEEEECCEECCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA