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The map label for this gene is ntrX [H]

Identifier: 161870939

GI number: 161870939

Start: 2076517

End: 2077647

Strand: Reverse

Name: ntrX [H]

Synonym: NMCC_2033

Alternate gene names: 161870939

Gene position: 2077647-2076517 (Counterclockwise)

Preceding gene: 161870940

Following gene: 161870938

Centisome position: 96.48

GC content: 56.85

Gene sequence:

>1131_bases
ATGGTGCTGCTGGATATTTGGATGCCTGATTGCGACGGCATCACCCTTTTGAAGGAGTGGGCGAAAAACGGGCAGCTCAA
TATGCCGGTGGTGATGATGAGCGGGCATGCCAGCATCGATACCGCCGTGGAAGCCACCAAAATCGGTGCGCTCGACTTTT
TGGAAAAACCGATTTCCCTGCAAAAGCTGCTGTCCACGGTTGAAAACGCGTTGAAGTACGGTGCGGCGCAAACCGAAACG
GGGCCTGTATTCGACAAGCTGGGCAACAGTGCGGCGATTCAGGAAATGAACCGTGAGGTAGGGGCTGCGGTGAAACGTGC
CTCTCCCGTACTTTTGACGGGCGAGGCAGGTTCGCCGTTTGAAACGGTGGCACGCTATTTCCATAAAAACGGTACGCCGT
GGGTCAGCCCGGCAAGGGTCGAATATCTGATCGATATGCCGATGGAACTGTTGCAGAAGGCGGAGGGCGGCGTTTTGTAT
GTCGGCGACATAGCCCAATACAGCCGCAACATCCAAGCCGGCATTGCCTTTATTGTCGGCAAGGCAGAACGCTGCCGCGT
CAGGGTGGTCGCATCGGGCAGCAGGGCGGCAGGTTCAGACGGCATTGCCTGCGAGGAAAAGCTGGCGGAACTGCTGTCGG
AATCGGTCGTCCGTATTCCGCCGCTGCGTATGCAGCATGAAGATATTCCCTTCCTGATACAGGGGATTGCCTGCAATGTG
GCGGAAAGCCAAAAGATTGCGCCCGCCGCATTCAGTGAAGATGCGCTTGCCGTGCTGACCCGTTACGACTGGCCGGGAAA
TTTCGACCAACTGCAAAGCGTCGTTGCAACGCTGTTGTTGGAGGCGGACGGACAGGAAATCGGCGCGGCGGCGGTTTCTT
CCCTTTTGGGGCAGAATGTGCCTGCCGAGGGAGCAGAAGATATGGTGGGCGGGTTTAATTTCAACCTGCCCCTGCGCGAA
TTGAGGGAGGAGGTGGAGCGGCGTTATTTCGAGTACCACATCGCCCAAGAAGGTCAGAATATGAGCAAAGTGGCGCAGAA
AGTCGGTTTGGAACGCACGCACCTTTACCGCAAACTCAAACAGCTCGGCATCGGCGTTTCGCGCCGGGCGGGGGAAAAAA
CCGAAGAATAG

Upstream 100 bases:

>100_bases
CGACCTGCTGTCGGAAATCCTGCAGGACGAAGGTTATTCGGTCGCATTGGCGGAAAACGCCGAAGAGGCGCGCAAGCTGC
GCCATCAGGCGCGCCCCGCG

Downstream 100 bases:

>100_bases
GCCCGGACGGCCGGTTTTCCGGCTGCGGGCTTTTGTTTTCAGACGGCATTTGGTGCAAATGCCGTCTGAAATCGTAAGGG
GACGGATTTTATGACAGAGG

Product: nitrogen assimilation regulatory protein NtrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDFLEKPISLQKLLSTVENALKYGAAQTET
GPVFDKLGNSAAIQEMNREVGAAVKRASPVLLTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLY
VGDIAQYSRNIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHEDIPFLIQGIACNV
AESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQEIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRE
LREEVERRYFEYHIAQEGQNMSKVAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE

Sequences:

>Translated_376_residues
MVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDFLEKPISLQKLLSTVENALKYGAAQTET
GPVFDKLGNSAAIQEMNREVGAAVKRASPVLLTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLY
VGDIAQYSRNIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHEDIPFLIQGIACNV
AESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQEIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRE
LREEVERRYFEYHIAQEGQNMSKVAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE
>Mature_376_residues
MVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDFLEKPISLQKLLSTVENALKYGAAQTET
GPVFDKLGNSAAIQEMNREVGAAVKRASPVLLTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLY
VGDIAQYSRNIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHEDIPFLIQGIACNV
AESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQEIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRE
LREEVERRYFEYHIAQEGQNMSKVAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=422, Percent_Identity=26.0663507109005, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1790437, Length=402, Percent_Identity=27.8606965174129, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788550, Length=412, Percent_Identity=25.7281553398058, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788905, Length=398, Percent_Identity=25.6281407035176, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082117, Length=330, Percent_Identity=24.5454545454545, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40944; Mature: 40944

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDFLEKPISL
CEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHH
QKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVLLTGEAGSPF
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
ETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSRNIQAGIAFIVG
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHCHHHHHHHHEEC
KAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHEDIPFLIQGIACNV
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
AESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQEIGAAAVSSLLGQNV
CCCCCCCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSKVAQKVGLERTHLYRKLK
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHH
QLGIGVSRRAGEKTEE
HHCCCCHHCCCCCCCC
>Mature Secondary Structure
MVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDFLEKPISL
CEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHH
QKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVLLTGEAGSPF
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
ETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSRNIQAGIAFIVG
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHCHHHHHHHHEEC
KAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHEDIPFLIQGIACNV
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
AESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQEIGAAAVSSLLGQNV
CCCCCCCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSKVAQKVGLERTHLYRKLK
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHH
QLGIGVSRRAGEKTEE
HHCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]