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The map label for this gene is tufA1 [H]

Identifier: 161870918

GI number: 161870918

Start: 2051190

End: 2052374

Strand: Reverse

Name: tufA1 [H]

Synonym: NMCC_2008

Alternate gene names: 161870918

Gene position: 2052374-2051190 (Counterclockwise)

Preceding gene: 161870919

Following gene: 161870917

Centisome position: 95.31

GC content: 54.09

Gene sequence:

>1185_bases
ATGGCTAAGGAAAAATTCGAACGTAGCAAACCGCACGTAAACGTTGGCACCATCGGTCACGTTGACCATGGTAAAACCAC
CCTGACTGCCGCTTTGACTACTATTTTGGCTAAAAAATTCGGCGGTGCTGCAAAAGCTTACGACCAAATCGACAACGCAC
CCGAAGAAAAAGCACGCGGTATTACCATTAACACCTCGCACGTGGAATACGAAACCGAAACCCGCCACTACGCACACGTA
GACTGCCCGGGGCACGCCGACTACGTTAAAAACATGATTACCGGCGCCGCACAAATGGACGGTGCAATCCTGGTATGTTC
CGCAGCCGACGGCCCTATGCCGCAAACCCGCGAACACATCCTGCTGGCTCGCCAAGTAGGCGTACCTTACATCATCGTGT
TCATGAACAAATGCGACATGGTCGACGATGCCGAGCTGTTGGAACTGGTTGAAATGGAAATCCGCGACCTGCTGTCCAGC
TACGACTTCCCCGGCGACGACTGCCCGATTGTACAAGGTTCCGCACTGAAAGCCTTGGAAGGCGATGCCGCTTACGAAGA
AAAAATCTTCGAACTGGCTGCCGCATTGGACAGCTACATCCCGACTCCCGAGCGTGCCGTGGACAAACCGTTCCTGCTGC
CTATCGAAGACGTGTTCTCCATTTCCGGCCGCGGTACAGTAGTAACCGGCCGTGTAGAGCGCGGTATCATCCACGTTGGT
GACGAGATCGAAATCGTCGGTCTGAAAGAAACCCAAAAAACCACCTGTACCGGCGTTGAAATGTTCCGCAAACTGCTGGA
CGAAGGTCAGGCGGGCGACAACGTAGGCGTATTGCTGCGCGGTACCAAACGTGAAGACGTAGAGCGTGGTCAGGTATTGG
CCAAACCGGGTACTATCACTCCTCACACCAAGTTCAAAGCAGAAGTGTACGTACTGAGCAAAGAAGAGGGCGGCCGCCAT
ACCCCGTTCTTCGCCAACTACCGTCCCCAATTCTACTTCCGTACCACCGACGTAACCGGCGCGGTTACTTTGGAAGAAGG
TGTGGAAATGGTAATGCCGGGTGAGAACGTAACCATTACCGTAGAACTGATTGCGCCTATCGCTATGGAAGAAGGCCTGC
GCTTTGCGATTCGCGAAGGCGGCCGTACCGTGGGTGCCGGCGTGGTTTCTTCTGTTATCGCTTAA

Upstream 100 bases:

>100_bases
CCGTAAAGGCTAATCAGGCAAATAGGTCGTCTGAAAGGCTGAAATGATTTTTCAGACGGCATTGTTCTTTAATCGATCTT
TAATGTAAAGGAATTAGCTC

Downstream 100 bases:

>100_bases
TTGAAGGATATTGATAAATGGCAAACCAAAAAATCCGTATCCGCCTGAAAGCTTATGATTACGCCCTGATTGACCGTTCT
GCACAAGAAATCGTTGAAAC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGITINTSHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVG
DEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRH
TPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA

Sequences:

>Translated_394_residues
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGITINTSHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVG
DEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRH
TPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA
>Mature_393_residues
AKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSY
DFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGD
EIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHT
PFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.7088607594937, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=31.0344827586207, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=31.0344827586207, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=29.372197309417, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=29.9334811529933, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI194018522, Length=340, Percent_Identity=30.8823529411765, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI46094014, Length=326, Percent_Identity=30.9815950920245, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018520, Length=340, Percent_Identity=30.8823529411765, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194097354, Length=340, Percent_Identity=30.8823529411765, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=29.1021671826625, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4503507, Length=358, Percent_Identity=25.1396648044693, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157426893, Length=298, Percent_Identity=25.8389261744966, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI82546879, Length=436, Percent_Identity=22.9357798165138, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=32.5925925925926, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=84.010152284264, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=84.010152284264, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=335, Percent_Identity=29.5522388059701, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI48994988, Length=240, Percent_Identity=32.5, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789108, Length=317, Percent_Identity=29.3375394321767, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=55.1378446115288, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=45.0777202072539, Blast_Score=337, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=447, Percent_Identity=29.9776286353468, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=447, Percent_Identity=29.9776286353468, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=28.735632183908, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=28.735632183908, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=31.25, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=358, Percent_Identity=27.6536312849162, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=173, Percent_Identity=32.3699421965318, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=266, Percent_Identity=27.0676691729323, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=64.3037974683544, Blast_Score=516, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.1801801801802, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.1801801801802, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=26.9886363636364, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=338, Percent_Identity=29.2899408284024, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=263, Percent_Identity=27.3764258555133, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=254, Percent_Identity=27.9527559055118, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=57.7608142493639, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=57.7608142493639, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=50.6493506493506, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=30.7692307692308, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=30.7692307692308, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=30.7692307692308, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=30.7692307692308, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=29.9559471365639, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=29.9559471365639, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45550900, Length=348, Percent_Identity=29.5977011494253, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=30.3206997084548, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647048, Length=266, Percent_Identity=25.5639097744361, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24647046, Length=266, Percent_Identity=25.5639097744361, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42909; Mature: 42778

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCC
ITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALE
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHC
GDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEEEEECCCEEEEC
DEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTIT
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCCCCEEECCCCCC
PHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTIT
CCCCEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCCEEEEHHCCCEEEECCCCEEEE
VELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA
EEEEECHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCC
ITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALE
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHC
GDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEEEEECCCEEEEC
DEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTIT
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCCCCEEECCCCCC
PHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTIT
CCCCEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCCEEEEHHCCCEEEECCCCEEEE
VELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA
EEEEECHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA