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The map label for this gene is gidB [H]

Identifier: 161870873

GI number: 161870873

Start: 2013391

End: 2014014

Strand: Reverse

Name: gidB [H]

Synonym: NMCC_1961

Alternate gene names: 161870873

Gene position: 2014014-2013391 (Counterclockwise)

Preceding gene: 161870874

Following gene: 161870872

Centisome position: 93.53

GC content: 56.89

Gene sequence:

>624_bases
ATGGAACGTAAAGAACGCCTGCGTGCAGGCATTGCCGCGATGGGGCTGGATATTTTGGAAACGGCGCAGGACAGGCTTTT
GGCCTATGTGGATTTGTTGAAAAAGTGGAACAAAACCTACAATCTGACCGCACTGCGCGACGAGGAAAAAATGATTGTCC
ATCATCTTTTGGACAGCCTGACGCTGCTGCCCTATATCGAAGGCGCACAGACGATGCTGGATGTCGGTTCGGGCGGCGGC
CAGCCCGGCATTCCGGCGGCGGTGTGCCGTCCGGATGTGCAGATAACCCTTTTGGATGCGAATACGAAGAAAACGGCTTT
TTTACAGCAGGCGGTTATCGAGTTGGGATTGGACAATGTGCGCGTGGTATCCGGACGCGTGGAGGCGGTTTCGGACGTGC
GTGCCGACGTGATTACCAGCCGTGCGTTTGCAGAACTGGCGGATTTTGTGTCGTGGACGGGGCATCTGTTGAAAGACGGC
GGCTACTGGGCGGCGATGAAGGGCGTGTATCCGCAGGGGGAAATCGACCGCCTGCCGCAGGATGTGTGCGTCGAAAAAGT
CCAAAGGCTTGACGTGCCGGGCTTGGATGCGGAACGCCATATCGCCATACTCCGCAAGCGTTGA

Upstream 100 bases:

>100_bases
AATAGGTTGGAACGGGAATGCCGTCTGAAACGCCGACACGCGGTTTCAGACGGCATGTTTTTCCGTTAACATTACGCCTG
AATATGGATAGGAAGCAGAT

Downstream 100 bases:

>100_bases
GCGCGCTTCAGACGGCATGAATACCTTTTTTGTGCGGATAAAGGTAAAATTCCGCACTGTTTTTCTTTTTTCAACATCAG
ACGGGACACGGGCGGGACAT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MERKERLRAGIAAMGLDILETAQDRLLAYVDLLKKWNKTYNLTALRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGG
QPGIPAAVCRPDVQITLLDANTKKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVITSRAFAELADFVSWTGHLLKDG
GYWAAMKGVYPQGEIDRLPQDVCVEKVQRLDVPGLDAERHIAILRKR

Sequences:

>Translated_207_residues
MERKERLRAGIAAMGLDILETAQDRLLAYVDLLKKWNKTYNLTALRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGG
QPGIPAAVCRPDVQITLLDANTKKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVITSRAFAELADFVSWTGHLLKDG
GYWAAMKGVYPQGEIDRLPQDVCVEKVQRLDVPGLDAERHIAILRKR
>Mature_207_residues
MERKERLRAGIAAMGLDILETAQDRLLAYVDLLKKWNKTYNLTALRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGG
QPGIPAAVCRPDVQITLLDANTKKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVITSRAFAELADFVSWTGHLLKDG
GYWAAMKGVYPQGEIDRLPQDVCVEKVQRLDVPGLDAERHIAILRKR

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=192, Percent_Identity=44.7916666666667, Blast_Score=178, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 22916; Mature: 22916

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKERLRAGIAAMGLDILETAQDRLLAYVDLLKKWNKTYNLTALRDEEKMIVHHLLDSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHH
TLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANTKKTAFLQQAVIELGLDNV
HHHHHHCCHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCE
RVVSGRVEAVSDVRADVITSRAFAELADFVSWTGHLLKDGGYWAAMKGVYPQGEIDRLPQ
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCH
DVCVEKVQRLDVPGLDAERHIAILRKR
HHHHHHHHHCCCCCCCCHHHHHHEECC
>Mature Secondary Structure
MERKERLRAGIAAMGLDILETAQDRLLAYVDLLKKWNKTYNLTALRDEEKMIVHHLLDSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHH
TLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANTKKTAFLQQAVIELGLDNV
HHHHHHCCHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCE
RVVSGRVEAVSDVRADVITSRAFAELADFVSWTGHLLKDGGYWAAMKGVYPQGEIDRLPQ
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCH
DVCVEKVQRLDVPGLDAERHIAILRKR
HHHHHHHHHCCCCCCCCHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA