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The map label for this gene is folA [H]

Identifier: 161870762

GI number: 161870762

Start: 1880728

End: 1881318

Strand: Reverse

Name: folA [H]

Synonym: NMCC_1836

Alternate gene names: 161870762

Gene position: 1881318-1880728 (Counterclockwise)

Preceding gene: 161870763

Following gene: 161870760

Centisome position: 87.36

GC content: 53.98

Gene sequence:

>591_bases
GTGCAAACAAAAAACGTATGGCGCGCGCCGGTTGAGATTTTTGACACGGAATGTCATAAAATGCCGTCTGAAGCGTTCAG
ACGGCATTTTTGTGGAGGAAATATGCTCAAAATAACCCTAATTGCGGCGTGTGCGGAAAACCTGTGCATCGGGGCGGGCA
ATGCTATGCCTTGGCACATCCCCGAAGATTTCGCATTTTTCAAAGCCTATACCTTGGGCAAACCCGTCATTATGGGGCGG
AAAACGTGGGAATCCCTGCCCGTCAAACCCCTGCCCGGACGGAGGAACATCGTCATCAGCCGGCAGGCGGATTATTGCGC
GGCAGGCGCGGAAACGGCGGCAAGTTTGGAGGCGGCATTGGCATTGTGCGCAGGCGCGGAAGAAGCTGTCATTATGGGCG
GCGCGCAGATATACGGACAAGCGATGCCATTGGCGACCGATTTGCGGATAACCGAAGTGGATTTGTCTGTGGAAGGAGAT
GCATTTTTCCCCGAAATAGACCGGACGCATTGGAAAGAGGCAGGGCGGACGGAACGCCGCGTCAGCAGCAAAGGCACGCG
CTATGCTTTTGTGCATTATTTGAGATATTGA

Upstream 100 bases:

>100_bases
GTCGAAGGCAGGCAGGACAAGCCGGAAGTGTACGGCAAGAGCATTACCGATGCGTGTATCGGTTGGGGCGCGACTGAAGA
ACTGTTGGCATTGTTGGCAG

Downstream 100 bases:

>100_bases
AATATAAACTCTCTATAAAATCCCCCGCAAATGATGGGCTGAAATAGAAAATATTGTTATTCCCCCGAAGATGGGAATCC
GGGATTTTAAAGTTAGGGTA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MQTKNVWRAPVEIFDTECHKMPSEAFRRHFCGGNMLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIMGR
KTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQIYGQAMPLATDLRITEVDLSVEGD
AFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYLRY

Sequences:

>Translated_196_residues
MQTKNVWRAPVEIFDTECHKMPSEAFRRHFCGGNMLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIMGR
KTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQIYGQAMPLATDLRITEVDLSVEGD
AFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYLRY
>Mature_196_residues
MQTKNVWRAPVEIFDTECHKMPSEAFRRHFCGGNMLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIMGR
KTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQIYGQAMPLATDLRITEVDLSVEGD
AFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYLRY

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=176, Percent_Identity=35.7954545454545, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI28827793, Length=156, Percent_Identity=35.8974358974359, Blast_Score=70, Evalue=9e-13,
Organism=Escherichia coli, GI1786233, Length=135, Percent_Identity=45.1851851851852, Blast_Score=122, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17505899, Length=175, Percent_Identity=32.5714285714286, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116008026, Length=148, Percent_Identity=34.4594594594595, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24647458, Length=148, Percent_Identity=34.4594594594595, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 21743; Mature: 21743

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKNVWRAPVEIFDTECHKMPSEAFRRHFCGGNMLKITLIAACAENLCIGAGNAMPWHI
CCCCCCHHCCHHHHCCHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCEEECCCCCCCCC
PEDFAFFKAYTLGKPVIMGRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAAL
CCHHHHHHHHHCCCCEEECCCHHHCCCCCCCCCCCCEEEECCCCCHHCCCHHHHHHHHHH
ALCAGAEEAVIMGGAQIYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERR
HHHCCCCCEEEECCHHHHCCCCCEEECCEEEEEEEEECCCCCCCCCCHHHHHHCCCHHHH
VSSKGTRYAFVHYLRY
HHCCCCEEEEEEEECC
>Mature Secondary Structure
MQTKNVWRAPVEIFDTECHKMPSEAFRRHFCGGNMLKITLIAACAENLCIGAGNAMPWHI
CCCCCCHHCCHHHHCCHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCEEECCCCCCCCC
PEDFAFFKAYTLGKPVIMGRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAAL
CCHHHHHHHHHCCCCEEECCCHHHCCCCCCCCCCCCEEEECCCCCHHCCCHHHHHHHHHH
ALCAGAEEAVIMGGAQIYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERR
HHHCCCCCEEEECCHHHHCCCCCEEECCEEEEEEEEECCCCCCCCCCHHHHHHCCCHHHH
VSSKGTRYAFVHYLRY
HHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]