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The map label for this gene is queF [H]

Identifier: 161870753

GI number: 161870753

Start: 1869691

End: 1870293

Strand: Direct

Name: queF [H]

Synonym: NMCC_1827

Alternate gene names: 161870753

Gene position: 1869691-1870293 (Clockwise)

Preceding gene: 161870747

Following gene: 161870754

Centisome position: 86.82

GC content: 51.41

Gene sequence:

>603_bases
ATGACACGGAAAACGCCGCCGTCCAAACCATGCCGTCTGAAGAAAACTACACAGATACCGCCGCCTGTATTACAATCGCC
GCCCCGTGGTTCGAAAACCTCCCACATTAAAAAACTAAGGAAACCCCCTATGTCCCGCAATACCGAAGAGCTGCAAGGCA
TCTCGCTTTTGGGTAATCAGAAAACCCAATATCCGATCGGCTACGCGCCTGAAATTCTCGAAGCATTCGACAACAAACAT
CCCGACAACGACTATTTCGTCAAATTCGTCTGCCCAGAGTTCACCAGCCTCTGCCCCATGACCGGGCAGCCCGACTTCGC
CACCATCGTCATCCGCTACATTCCGCACATCAAAATGGTGGAAAGCAAATCCCTGAAACTCTACCTCTTCAGCTTCCGCA
ACCACGGCGATTTTCATGAAGACTGTGTCAACATCATCATGAAAGACCTCATTGCCCTGATGGATCCGAAATACATCGAA
GTATTCGGCGAGTTCACACCGCGCGGCGGCATCGCCATTCATCCGTTTGCCAACTACGGCAAAGCAGGCACCGAGTTTGA
AGCATTGGCGCGTAAACGCCTGTTTGAGCACGACGCACAATAA

Upstream 100 bases:

>100_bases
GTACGGAACCGATTCACTTAGTGCTTCAGCACCTTAGAGAATCGTTCTCTTTGAGCTAAGGCGAGGCAACGCCGTACTGG
TTTTTGTTAATCCACTATAT

Downstream 100 bases:

>100_bases
ACCATCCTTCCCACTTTCAGACGGCATTCCTTCGATACGATATGCCGTCTGAACGCCTTTCCGAAAGATTTTTATGTACG
CATTTACCGACGCACAGCAA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MTRKTPPSKPCRLKKTTQIPPPVLQSPPRGSKTSHIKKLRKPPMSRNTEELQGISLLGNQKTQYPIGYAPEILEAFDNKH
PDNDYFVKFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNIIMKDLIALMDPKYIE
VFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ

Sequences:

>Translated_200_residues
MTRKTPPSKPCRLKKTTQIPPPVLQSPPRGSKTSHIKKLRKPPMSRNTEELQGISLLGNQKTQYPIGYAPEILEAFDNKH
PDNDYFVKFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNIIMKDLIALMDPKYIE
VFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ
>Mature_199_residues
TRKTPPSKPCRLKKTTQIPPPVLQSPPRGSKTSHIKKLRKPPMSRNTEELQGISLLGNQKTQYPIGYAPEILEAFDNKHP
DNDYFVKFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNIIMKDLIALMDPKYIEV
FGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=113, Percent_Identity=32.7433628318584, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 22837; Mature: 22706

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKTPPSKPCRLKKTTQIPPPVLQSPPRGSKTSHIKKLRKPPMSRNTEELQGISLLGNQ
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
KTQYPIGYAPEILEAFDNKHPDNDYFVKFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMV
CCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCEEEE
ESKSLKLYLFSFRNHGDFHEDCVNIIMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYG
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEECCCCCCC
KAGTEFEALARKRLFEHDAQ
CCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRKTPPSKPCRLKKTTQIPPPVLQSPPRGSKTSHIKKLRKPPMSRNTEELQGISLLGNQ
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
KTQYPIGYAPEILEAFDNKHPDNDYFVKFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMV
CCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCEEEE
ESKSLKLYLFSFRNHGDFHEDCVNIIMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYG
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEECCCCCCC
KAGTEFEALARKRLFEHDAQ
CCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA